Immunohistochemical Recognition of Human Follicular Dendritic Cells (FDCs) in Routinely Processed Paraffin Sections

Abstract

A number of monoclonal antibodies (MAbs) that recognize human follicular dendritic cells (FDCs) have been identified. Although some of them have already been applied individually in routine immunolabeling using formalin-fixed paraffin sections for diagnostic and experimental purposes, many antibodies are still employed only for immunolabeling using cryostat sections or particularly processed sections because they have been thought unsuitable for routine sections. A comprehensive examination re-evaluating their suitability in paraffin sections has not been reported. Accordingly, there is limited ability to examine the immunopathological contribution or diagnostic value of FDCs using routinely processed specimens or archived materials. In this study a broad panel of antibodies was systematically applied to the immunolabeling of paraffin sections of reactive tonsils or lymph nodes, in combination with advanced antigen retrieval (AR) techniques. Several antibodies, including Ki-M4p, X-11, 12B1, CNA.42, 1F8/BU32 (anti-CD21), BU38/1B12 (anti-CD23), Ber-MAC-DRC/To5 (anti-CD35), 1.4C3 (anti-CD106), NGFR5 (anti-nerve growth factor receptor p75), IIH6 (anti-CD55), 55K-2 (anti-fascin), and anti-S100 protein alpha-chain, were found to label FDCs in routine sections when combined with suitable AR techniques. Our results are easily adaptable for routine practice and provided useful suggestions concerning the immunopathological behavior and diversity of the particular cells.

Development, maturation and subsequent activation of follicular dendritic cells (FDC): immunohistochemical observation of human fetal and adult lymph nodes

“…Recently, we established that a number of monoclonal antibodies recognizing human FDC were also available for immunolabeling of formalin fixed, routinely processed paraffin sections when combined with suitable antigen retrieval techniques (Maeda et al 2002). Based upon this information, a suitable antigen retrieval method was selected for each antibody from the following methods (Table 1): 1.…”

“…Such preparations have been considered essential for immunohistochemical identification of human FDC or related cells. We have recently established that a number of the monoclonal antibodies that recognize human FDC can be used in routinely processed paraffin sections when suitable antigen retrieval methods are employed (Maeda et al 2002). Using such techniques, we examined different gestational stages of fetal lymph nodes (FLN) from autopsy materials, which had been embedded in paraffin blocks and were archived at the Department of Pathology, Yamagata University School of Medicine, for routine histological investigation, in order to define the process of development, maturation, and activation of human FDC.…”

Immunohistochemical Detection of Dendritic Cell Markers in Cattle

“…10 In this study, we used a monoclonal antibody (clone CNA.42) for the labeling of FDCs 34 and a polyclonal antibody for the detection of S100 protein, which is expressed in a wide variety of cell types, including FDCs. 17,23 Because of the complicated interactions between the distinct cells associated with both normal and pathologic mechanisms of action of the immune system, it is of critical interest to examine DCs not only in cell culture but also in vivo. This study aims both to standardize the immunohistochemical method for the detection of different potential DC markers but also to elucidate the histological distribution of these markers, some of which are commonly used in many in vitro studies, thus helping to expand the understanding of the various roles of DCs in different cattle diseases.…”

18F-FDG Uptake in Reactive Neck Lymph Nodes of Oral Cancer: Relationship to Lymphoid Follicles

“…Lymphoid follicles are composed of lymphocytes, macrophages, and follicular dendritic cells (FDCs). Networks of these cells in lymphoid follicles make up germinal centers, which can easily be seen on histologic sections stained with hematoxylin and eosin (H&E) (10) and can be differentiated by immunohistochemical methods; that is, B lymphocytes by CD79a (11), T lymphocytes by CD3 (12), macrophages by CD68 (13), and FDCs by CD21 (14).…”