Immunohistochemical studies on the bovine lactating mammary gland (Bos taurus)

Alkafafy, Mohamed; Rashed, Reda; Helal, Amr M.

doi:10.1016/j.acthis.2011.02.012

Cited by 17 publications

(17 citation statements)

References 31 publications

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“…The bovine mammary myoepithelial cells are characterized by expression of α-SMA 42, 56, 57, 76, vimentin 76, CK6, CK14, CK18, connexin-43 (Cx43) 42, 57, p63 and CD49f 42. However, Hellmen and Isaksson 56 reported that CK14 is a less specific marker for bovine myoepithelial cells than α-SMA, while a recent study claimed a strong expression of CK14 in bovine myoepithelial cells 57.…”

Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

“…However, Hellmen and Isaksson 56 reported that CK14 is a less specific marker for bovine myoepithelial cells than α-SMA, while a recent study claimed a strong expression of CK14 in bovine myoepithelial cells 57. CK18 is a potent lineage marker of bovine mammary duct compartment 42, 56, 57.…”

Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

“…CK18 is a potent lineage marker of bovine mammary duct compartment 42, 56, 57. The expression of cytokeratin and smooth muscle actin markers on myoepithelial cells indicate the close similarity with smooth muscle cells 57, 77. Bovine myoepithelial cells express the common acute lymphoblastic leukemia antigen (CALLA), also known as CD10 and neutral endopeptidase 58.…”

Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

“…in humans and mouse, while there is limited work in bovines. Vascular endothelial growth factor (VEGF) is a heparin-binding growth factor specific for vascular endothelial cells and VEGF receptor is used as endothelial stem/progenitor cell marker 57. The severe inflammatory process during mastitis results in extensive damage to the vascular network in the bovine mammary gland 45.…”

Section: Stem Cells From Other Originmentioning

confidence: 99%

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Stem Cell Research: A Novel Boulevard towards Improved Bovine Mastitis Management

Sharma

Jeong

2013

Int. J. Biol. Sci.

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The dairy industry is a multi-billion dollar industry catering the nutritional needs of all age groups globally through the supply of milk. Clinical mastitis has a severe impact on udder tissue and is also an animal welfare issue. Moreover, it significantly reduces animal value and milk production. Mammary tissue damage reduces the number and activity of epithelial cells and consequently contributes to decreased milk production. The high incidence, low cure rate of this highly economic and sometimes deadly disease is an alarming for dairy sector as well as policy makers. Bovine mammary epithelial cells (MECs) and their stem cells are very important in milk production and bioengineering. The adult mammary epithelium consists of two main cell types; an inner layer of luminal epithelial cells, which produce the milk during lactation, and an outer layer of myoepithelial cells resting on a basement membrane, which are responsible for pushing the milk through the ductal network to the teat cistern. Inner layer of columner/luminal cells of bovine MECs, is characterized by cytokeratin18, 19 (CK18, CK19) and outer layer such as myoepithelial cells which are characterized by CK14, α-smooth muscle actin (α-SMA) and p63. Much work has been done in mouse and human, on mammary gland stem cell research, particularly in cancer therapy, but stem cell research in bovine is still in its infancy. Such stem/progenitor cell discoveries in human and mouse mammary gland bring some hope for application in bovines. These progenitors may be therapeutically adopted to correct the structural/cytological defects in the bovine udder due to mastitis. In the present review we focused on various kinds of stem/progenitor cells which can have therapeutic utility and their possibilities to use as a potential stem cell therapy in the management of bovine post-mastitis damage in orders to restore milk production. The possibilities of bovine mammary stem cell therapy offers significant potential for regeneration of tissues that can potentially replace/repair diseased and damaged tissue through differentiation into epithelial, myoepithelial and/or cuboidal/columnar cells in the udder with minimal risk of rejection and side effects.

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Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

Section: Mammary Gland Stem/progenitor Cellsmentioning

confidence: 99%

Section: Stem Cells From Other Originmentioning

confidence: 99%

See 2 more Smart Citations

Stem Cell Research: A Novel Boulevard towards Improved Bovine Mastitis Management

Sharma

Jeong

2013

Int. J. Biol. Sci.

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“…The most recent methodologies, viz. morphology, histopathology (Alkafafy, Rashed, & Helal, 2012;Kaushik et al, 2013;Safayi et al, 2012), bromodeoxyuridine retention (Capuco, 2007), Hoechst dye-effluxing to identify side population (SP) properties and surface markers by flow cytometer or immunochemistry (Rauner & Barash, 2012;VanKeymeulen et al, 2011), are being used for the characterization of bovine mammary epithelial stem/progenitor cells. We isolated the Korean Hosltein mammary epithelial stem cells (KHMESCs) from Korean Holstein mammary tissue using collagenase.…”

Section: Introductionmentioning

confidence: 99%

Differentiation dynamics of mammary epithelial stem cells from Korean holstein dairy cattle under ECM-free conditions

Sharma

Kim

Sodhi

et al. 2015

Journal of Biomolecular Structure and Dynamics

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The "stem cells" are commonly defined as "cells capable of self-renewal through replication and differentiating into specific lineages". The mammary gland contains functional stem/progenitor cells. The current study was planned with the objectives to study the differentiation dynamics of Korean Holstein mammary epithelial stem cells (KHMESCs) under the optimum culture conditions. Lineage negative KHMESCs isolated from mammary tissue of lactating cows have shown the typical differentiation dynamics with formation of lobulo-alveolar structures in in vitro culture. This suggests the existence of bipotential mammary epithelial stem cells in the mammary gland. The strong mRNA expression of pluripotency factors indicates stemness, whereas expression of milk protein genes and epithelial cell-specific gene indicate their differentiation capabilities. Further, immunostaining results have shown the differentiation capabilities of KHMESCs into both luminal and basal lineages under the extracellular matrix (ECM, matrigel) free environment. However, under matrigel, the differentiation process was comparatively higher than without matrigel. Immunostaining results also suggested that differentiated cells could secrete milk proteins such as β-casein. To our knowledge, these data represent the first report on the differentiation dynamics and establishment of mammary epithelial stem cells from Korean Holstein with typical stemness properties. It was observed that isolated KHMESCs had normal morphology, growth pattern, differentiation ability, cytogenetic and secretory activity even without ECM. Therefore, it is concluded that established KHMESCs could be used for further studies on Korean Holstein dairy cows related to lactation studies, as non-GMO animal bioreactors and stem cell-based management of bovine mastitis including post-mastitis damage.

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Immunohistochemical studies on the poll gland of the dromedary camel (Camelus dromedarius) during the rutting season

2012

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Immunohistochemical studies on the bovine lactating mammary gland (Bos taurus)

Cited by 17 publications

References 31 publications

Stem Cell Research: A Novel Boulevard towards Improved Bovine Mastitis Management

Stem Cell Research: A Novel Boulevard towards Improved Bovine Mastitis Management

Differentiation dynamics of mammary epithelial stem cells from Korean holstein dairy cattle under ECM-free conditions

Immunohistochemical studies on the poll gland of the dromedary camel (Camelus dromedarius) during the rutting season

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