Immunohistochemical study of purulent wounds treated with King crab collagenase

Abstract

Immunohistochemical study of tissues from purulent wounds in rats after treatment with the collagenase isolated from the King crab Paralithodes camtschatica was undertaken. The enzymotherapy resulted in a rapid and efficient removal of necrotic debris. It was accompanied by fibrin elimination from the wound bed and subsequent formation of new capillaries. Cellular fibronectin with ED-A sequence was identified in the newly formed granulation tissue, which points to its active synthesis in situ. Polyclonal antibodies against two isozymes of the crab collagenolytic protease were obtained. By their use it was shown that, after application of the collagenase, both isozymes accumulated in fibrin deposits at the wound bed but did not penetrate adherent granulation tissue.

Autophagy Therapeutic Potential of Garlic in Human Cancer Therapy

“…Hence to find more advanced therapy, several researchers try to identify novel materials bestowed in nature. Many natural components have incredible potential to cure diseases without any adverse side effects, such as the collagenase isolated from the King crab ( Paralithodes camtschatica ) as a the strongest antibiotic,[1] the alkaloids from the skin of poison dart frogs (Dendrobatidae) toward the development of chemical defense. [2]…”