Immunohistochemical study on neuropilin 1 (NRP1) immunoexpression in oral squamous cell carcinoma

Stasikowska-Kanicka, Olga; Wągrowska-Danilewicz, Małgorzata; Danilewicz, Marian

doi:10.5603/fhc.a2018.0010

Cited by 7 publications

(5 citation statements)

References 37 publications

(44 reference statements)

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“…We have also previously demonstrated that M2 macrophages contribute to the process of angiogenesis in the esophageal squamous cell carcinoma microenvironment [18]. In addition, several researchers have reported that CD163 + macrophages promote angiogenesis in oral squamous cell carcinoma [31][32][33]. On the other hand, to our knowledge, there is a lack of reports on angiogenesis in OVX.…”

Section: Discussionmentioning

confidence: 87%

CD163+ Foamy Macrophages Are Associated with the Morphogenesis of Oral Verruciform Xanthoma through Angiogenesis by VEGF Expression: An Immunohistochemical Study

et al. 2020

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Oral verruciform xanthoma (OVX) is an uncommon benign lesion that is characterized histologically by the accumulation of several foamy macrophages in the lamina propria papillae. The pathogenesis of OVX has not been completely elucidated, although the significance of macrophage polarization (M1, tumor suppression; and M2, tumor promotion) and the contribution of M2 macrophages to angiogenesis are well established. This study investigated the role of foamy macrophages in OVX, with a focus on angiogenesis. Four patients who underwent surgical excision or total excisional biopsy for OVXs were enrolled in this study. We evaluated the expression of the macrophage markers CD68 (broad) and CD163 (M2) and the CD34-positive microvessel density (MVD) of OVXs. The foamy macrophages of all patients exhibited positivity to CD68 and CD163. We evaluated the MVD and the expression of the vascular endothelial growth factor (VEGF) based on histological architecture. The MVD of all OVX cases was significantly higher than that of the corresponding normal epithelia. Interestingly, the MVD of verrucous-type OVX cases was higher than that of the other type. VEGF was expressed on foamy macrophages in all cases. Overall, the foamy macrophages expressing CD163 were associated with the morphogenesis of OVX through the process of angiogenesis by VEGF expression.

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Section: Discussionmentioning

confidence: 87%

CD163+ Foamy Macrophages Are Associated with the Morphogenesis of Oral Verruciform Xanthoma through Angiogenesis by VEGF Expression: An Immunohistochemical Study

et al. 2020

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“…Neuropilins were also shown to engage in tumor neovascularization and metastasis, and their co-expression was shown to be correlated with the increased vascularity and poor prognosis for NSCLC patients [ 58 ]. The expression of both NRP1 and NRP2 was found in many tumor types [ 59 ], where their overexpression contributed to metastasis [ 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Catulin Based Reporter System to Track and Characterize the Population of Invasive Cancer Cells in the Head and Neck Squamous Cell Carcinoma

Karpińska

Gielata

Gwiazdowska

et al. 2021

IJMS

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Head and neck squamous cell carcinoma (HNSCC) is an aggressive tumor with a poor prognosis due to late diagnosis and loco-regional metastasis. Partial or more complete epithelial–mesenchymal transition (EMT) plays a role in tumor progression; however, it remains a challenge to observe the EMT in vivo, due to its transient nature. Here, we developed a novel catulin promoter-based reporter system that allows us to isolate and characterize in vivo a small fraction of invasive cancer cells. The analyses of tumors revealed that Catulin-green fluorescent protein (GFP)-positive cells were enriched in clusters of cells at the tumor invasion front. A functional genomic study unveiled genes involved in cellular movement and invasion providing a molecular profile of HNSCC invasive cells. This profile overlapped partially with the expression of signature genes related to the partial EMT available from the single cell analysis of human HNSCC specimens, highlighting the relevance of our data to the clinical disease progression state. Interestingly, we also observed upregulations of genes involved in axonal guidance—L1 cell adhesion molecule (L1CAM), neuropilin-1, semaphorins, and ephrins, indicating potential interactions of cancer cells and neuronal components of the stroma. Taken together, our data indicated that the catulin reporter system marked a population of invasive HNSCC cells with a molecular profile associated with cancer invasion.

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“…Neuropilin-1 (NRP1) is a transmembrane or cytosolic protein that acts on several signaling pathways, such as angiogenesis through VEGF and HGF in endothelial cells [ 48 ], and is a tumor promotor overexpressed in several tumor tissues [ 49 , 50 , 51 ]. Issit et al revealed a physical interaction between NRP1 and ABCB8.…”

Section: Heme Metabolism—a Complex Network Tightly Regulated By Enzym...mentioning

confidence: 99%

“…NRP1 is a tumor promotor overexpressed in several tumor tissues such as oral squamous cell carcinoma [ 49 ], breast [ 50 ] and lung [ 51 ] cancers. Interestingly, only one study was found to connect NRP1 to heme synthesis.…”

Section: Concerning Ppix Selective Accumulation In Neoplastic Cellsmentioning

confidence: 99%

A Recap of Heme Metabolism towards Understanding Protoporphyrin IX Selectivity in Cancer Cells

Kiening

Lange

2022

IJMS

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Mitochondria are essential organelles of mammalian cells, often emphasized for their function in energy production, iron metabolism and apoptosis as well as heme synthesis. The heme is an iron-loaded porphyrin behaving as a prosthetic group by its interactions with a wide variety of proteins. These complexes are termed hemoproteins and are usually vital to the whole cell comportment, such as the proteins hemoglobin, myoglobin or cytochromes, but also enzymes such as catalase and peroxidases. The building block of porphyrins is the 5-aminolevulinic acid, whose exogenous administration is able to stimulate the entire heme biosynthesis route. In neoplastic cells, this methodology repeatedly demonstrated an accumulation of the ultimate heme precursor, the fluorescent protoporphyrin IX photosensitizer, rather than in healthy tissues. While manifold players have been proposed, numerous discrepancies between research studies still dispute the mechanisms underlying this selective phenomenon that yet requires intensive investigations. In particular, we wonder what are the respective involvements of enzymes and transporters in protoporphyrin IX accretion. Is this mainly due to a boost in protoporphyrin IX anabolism along with a drop of its catabolism, or are its transporters deregulated? Additionally, can we truly expect to find a universal model to explain this selectivity? In this report, we aim to provide our peers with an overview of the currently known mitochondrial heme metabolism and approaches that could explain, at least partly, the mechanism of protoporphyrin IX selectivity towards cancer cells.

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Immunohistochemical study on neuropilin 1 (NRP1) immunoexpression in oral squamous cell carcinoma

Cited by 7 publications

References 37 publications

CD163+ Foamy Macrophages Are Associated with the Morphogenesis of Oral Verruciform Xanthoma through Angiogenesis by VEGF Expression: An Immunohistochemical Study

CD163+ Foamy Macrophages Are Associated with the Morphogenesis of Oral Verruciform Xanthoma through Angiogenesis by VEGF Expression: An Immunohistochemical Study

Catulin Based Reporter System to Track and Characterize the Population of Invasive Cancer Cells in the Head and Neck Squamous Cell Carcinoma

A Recap of Heme Metabolism towards Understanding Protoporphyrin IX Selectivity in Cancer Cells

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