Immunohistochemistry and ultrastructure of myoepithelium and modified myoepithelium of the ducts of human major salivary glands: Histogenetic implications for salivary gland tumors

Dardick, Irving; Rippstein, Peter; Skimming, Linda; Boivin, Marie; Parks, William; Dairkee, Shanaz H.

doi:10.1016/0030-4220(87)90173-3

Cited by 94 publications

(49 citation statements)

References 39 publications

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“…In addition, the myoepithelial cells in human breast, reported to be reactive for MT, may not necessarily represent the expression of MT in salivary myoepithelial cells which are structurally and functionally more heterogeneous. A consistent reactivity of MT in salivary tumors in non-luminal cells, the neoplastic myoepithelial cells or their counterparts, in particular, in the majority of the salivary tumors evaluated in the present study may have a potential implication in defining the role of neoplastic myoepithelial cells, believed by many investigators to have a major role in the histomorphology and histogenesis of salivary tumors [9]. As duct-like and /or myoepitheliallike cells are the predominant histopathological features of salivary neoplasms, a subpopulation of ductal cells located on the non-luminal aspect of the striated and excretory ducts of human salivary glands with a more heterogeneous and functionally more complex features have been identified by ultrastructural and immunohistochemical studies [7], which in the present study showed consistent immunoreactivity for MT.…”

Section: Discussionsupporting

confidence: 71%

Immunoreactive Metallothionein in Salivary Gland Neoplasms.

Shrestha

Takagi

Takai

et al. 1996

Acta Histochem. Cytochem

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Section: Discussionsupporting

confidence: 71%

Immunoreactive Metallothionein in Salivary Gland Neoplasms.

Shrestha

Takagi

Takai

et al. 1996

Acta Histochem. Cytochem

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“…Among the several subtypes of cytokeratin, cytokeratin 14 is expressed most consistently and is specific for myoepithelial and ductal basal cells (Dardick et al 1987). Here we show that normal human myoepithelial and basal ductal cells do react with antibodies to p63 protein.…”

Section: Discussionmentioning

confidence: 99%

“…Myoepithelial cells express ␣ -smooth muscle actin (Gugliotta et al 1988), smooth muscle myosin heavy chain (Longtine et al 1985), and calponin (Carmichael et al 1994), as well as cytokeratin 14 (Dardick et al 1987). They represent a basal compartment, which may host stem cells.…”

mentioning

confidence: 99%

p63 Is Expressed in Basal and Myoepithelial Cells of Human Normal and Tumor Salivary Gland Tissues

Bilal

Handra‐Luca

Bertrand

et al. 2003

J Histochem Cytochem.

131

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S U M M A R Y p63 is essential for epithelial cell survival and may function as an oncogene. We examined by immunohistochemistry p63 expression in human normal and tumor salivary gland tissues. In normal salivary glands, p63 was expressed in the nuclei of myoepithelial and basal duct cells. Among 68 representative salivary gland tumors, 63 displayed p63 reactivity. In all tumor types differentiated towards luminal and myoepithelial lineages (pleomorphic adenomas, basal cell adenomas, adenoid cystic carcinomas, and epithelialmyoepithelial carcinomas), p63 was expressed in myoepithelial cells, whereas luminal cells were always negative. Similarly, in mucoepidermoid carcinomas, basal, intermediate, and squamous cells expressed p63, in contrast to luminal mucous cells. p63 reactivity was also restricted to basal cells in Warthin tumors and oncocytomas. Myoepitheliomas and myoepithelial carcinomas all expressed p63. The only five negative tumors were three of four acinar cell carcinomas and two of three adenocarcinomas. In conclusion, p63 is expressed in the nuclei of normal human salivary gland myoepithelial and basal duct cells. p63 expression is retained in the modified myoepithelial and basal cells of human salivary gland tumors, which suggests a role for p63 in oncogenesis of these complex tumors.

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“…It is suggested that ductal basal cells may have a potentiality for repair of the ductal system. The histogenesis of salivary pleomorphic adenoma has been studied extensively by both light and electron microscopy (1,(10)(11)(12)(13). At the present time, two major theories have been proposed as to the progenitor cell of origin responsible for cellular FIG (14,18).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the histogeneses of salivary gland tumors have been evaluated to immunohistochemically detect ductal basal cells as specific staining to keratin proteins (3,8,12,14,18,27,33,34) and many monoclonal antibodies to keratins were decorated to ductal epithelial cells (5-7, 21-23, 31, 32). Preceding papers have reported that pleomorphic adenomas co-expressed keratin and vimentin (6,23,31), and that PKK1 and KL1 decorated luminal tumor cells of tubulo-ductal structures of such benign tumors (21,22).…”

Section: Monoclonal Antibodymentioning

confidence: 99%

Monoclonal antibody to keratin K8.12 expression in ductal basal cells of obstructive lesions and in outer tumor cells of salivary pleomorphic adenomas.

Shinohara

Yamada

Tanaka

et al. 1989

Acta Histochem. Cytochem

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Monoclonal antibodyfor keratin K8.12 (keratin nos. 13 and 16) was evaluated in paraffin sections of 17 cases of obstructive sialadenitis and 97 pleomorphic adenomas.In normal salivary glands fixed with 10% formalin, K8.12 staining was strongly limited to ductal basal cells, and staining to PKK1 and KL1 was positive in duct cells. Monoclonal antibodies PKK1 and KL1 were compared in duct-like structures of obstructive lesions and pleomorphic adenomas.Duct-like structures in obstructive lesions showed strong staining for K8.12 in ductal basal cells, and positive staining for PKK1 and KL1 in ductal epithelial cells, in different stages of ductal obstruction.Pleomorphic adenomas showed an intense expression for K8.12 staining in outer tumor cells of tubulo-ductal structures, and positive reaction for PKK1 and KL1 in luminal tumor cells. The histogenesis of pleomorphic adenoma is discussed in terms of the immunohistochemical labelling of the 3 types of monoclonal antibodies to keratin.The identification of a progenitor cell to ductal basal cells is also hypothesized.Salivary glands are composed of terminal acinar components and ductal segments, and ductal epithelium also consists of ductal basal cells and luminal cells. Recently, the histogeneses of salivary gland tumors have been evaluated to immunohistochemically detect ductal basal cells as specific staining to keratin proteins (3,8,12,14,18,27,33,34) and many monoclonal antibodies to keratins were decorated to ductal epithelial cells (5-7, 21-23, 31, 32). Preceding papers have reported that pleomorphic adenomas co-expressed keratin and vimentin (6,23,31), and that PKK1 and KL1 decorated luminal tumor cells of tubulo-ductal structures of such benign tumors (21,22). The present study describes the distribution of keratin stained by monoclonal antibody K8.12 (nos 13, 16) in obstructive sialadenitis and pleomorphic adenomas, which showed different staining patterns compared with those observed by staining with monoclonal antibodies PKK1 and KL1.

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Immunohistochemistry and ultrastructure of myoepithelium and modified myoepithelium of the ducts of human major salivary glands: Histogenetic implications for salivary gland tumors

Cited by 94 publications

References 39 publications

Immunoreactive Metallothionein in Salivary Gland Neoplasms.

Immunoreactive Metallothionein in Salivary Gland Neoplasms.

p63 Is Expressed in Basal and Myoepithelial Cells of Human Normal and Tumor Salivary Gland Tissues

Monoclonal antibody to keratin K8.12 expression in ductal basal cells of obstructive lesions and in outer tumor cells of salivary pleomorphic adenomas.

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