Immunohistochemistry of deparaffinised sections using antigen retrieval with microwave combined pressure cooking versus immunofluorescence in the assessment of human renal biopsies

Shu, Shi; Zhang, Ping; Cheng, Qingli; Wu, Jie; Cui, Jing; Zheng, Ying; Bai, Xueyuan; Chen, Xiangmei

doi:10.1136/jclinpath-2012-201125

Cited by 8 publications

(6 citation statements)

References 31 publications

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“…Consistent with previous reports, IF appears to be a more sensitive method for detecting linear IgG deposits [9,10]. It may therefore be the preferred diagnostic test when anti-GBM disease is suspected, although using antigen retrieval with microwave and pressure cooking may increase the sensitivity of IHC [11]. IF offers more rapid results on freshly frozen tissue sections while IHC can be performed with less tissue.…”

Section: Discussionsupporting

confidence: 81%

Hidden Tiger: An Atypical Presentation of Anti-Glomerular Basement Membrane Disease

Lecamwasam

2014

World J Nephrol Urol

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Anti-glomerular basement membrane (anti-GBM) disease is characterized by linear deposition of immunoglobulin G (IgG) along the GBM. The concurrence of anti-GBM disease with another type of glomerulonephritis has been well described. We report a case of necrotizing glomerulonephritis in association with prodromal systemic symptoms, deterioration in kidney function less rapid than expected, and the presence of circulating anti-GBM antibody. This was initially diagnosed histologically as immunoglobulin A (IgA) nephropathy based on immunohistochemistry (IHC) staining, but immunofluorescence (IF) staining on a subsequent biopsy was more consistent with a diagnosis of anti-GBM disease with coexisting mild IgA nephropathy. Prompt initiation of standard treatment of anti-GBM disease normalized kidney function. This case highlights that anti-GBM disease can present atypically and may also co-exist with another form of glomerulonephritis.

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Section: Discussionsupporting

confidence: 81%

Hidden Tiger: An Atypical Presentation of Anti-Glomerular Basement Membrane Disease

Lecamwasam

2014

World J Nephrol Urol

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“…[9][10][11][12][13] In other immune-mediated diseases, such as renal diseases, IHCA already constitute a well-established diagnostic procedure. [14][15][16] In this retrospective study, we have systematically assessed the value of IHCA using paraffin-embedded skin biopsy specimens obtained from all newly diagnosed cases of BP evaluated over 3 years.…”

Section: Discussionmentioning

confidence: 99%

“…For these reasons, a few studies have recently assessed the value of immunohistochemistry analyses (IHCA) on paraffin‐embedded tissue as an alternative for the diagnosis of autoimmune bullous skin diseases . In other immune‐mediated diseases, such as renal diseases, IHCA already constitute a well‐established diagnostic procedure …”

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confidence: 99%

Diagnostic value of immunohistochemistry on formalin-fixed, paraffin-embedded skin biopsy specimens for bullous pemphigoid

et al. 2016

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Funding  None Conflict of interest  None declared What's already known about this topic? Direct immunofluorescence microscopy (DIF) studies represent the gold standard for the diagnosis of bullous pemphigoid (BP). Recent studies have reported that immunohistochemistry techniques using formalin-fixed, paraffinembedded skin biopsy specimens are able to detect tissue-bound immunoreactants in BP with high sensitivity and thus constitute an alternative and practical diagnostic tool. What does this study add? Immunohistochemical analyses are not sensitive enough to recommend their use for the diagnosis of BP, since less than 50% of 51 consecutive paraffin-embedded skin biopsy specimens obtained from patients with newly diagnosed BP were positive. If DIF cannot be performed, immunohistochemistry represents a useful diagnostic tool only in presence of histologically subepidermal blistering and/or with positive immunoserological tests , which are associated with a better sensitivity. Manuscript words 2845, tables 2 and figures 2, Abstract 248Abstract Background: Direct immunofluorescence microscopy (DIF) studies constitute the gold standard for

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“…Tumors from NSCLC carcinoma xenograph mice treated with AbMTA2 or PBS were excised on day 30 and fixed using 10% formaldehyde followed by embedding in paraffin wax, and cut into serial sections (4 µm). Tissues were washed with PBS-Tween-20 (PBST) three times at room temperature and antigen retrieval was performed on the tumor sections using a microwave heating AR ( 43 ) subsequent to a series of ethanols (100, 95 and 80%). Tumor sections were washed with PBST at room temperature and incubated with primary antibodies: EGF (1:500; cat.…”

Section: Methodsmentioning

confidence: 99%

Metastasis-associated protein 2 promotes the metastasis of non-small cell lung carcinoma by regulating the ERK/AKT and VEGF signaling pathways

2018

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Non-small cell lung carcinoma (NSCLC) is the most common cause of cancer-associated mortality in the world and accounts for ~85% of human lung cancers. Metastasis-associated protein 2 (MTA2) is a component of the histone deacetylase complex and serves a role in tumor progression; however, the mechanism through which MTA2 is involved in the progression of NSCLC remains unclear. The aim of the present study was to investigate the expression and function of MTA2 and the MTA2-mediated signaling pathway in NSCLC cells. Expression of MTA2 and its target genes was analyzed in MTA2-overexpressing and anti-MTA2 antibody (AbMTA2)-treated NSCLC cells, as well as growth, migration, invasion and apoptotic-resistance. The inhibitory effects on tumor formation were analyzed using AbMTA2-treated NSCLC cells and in a mouse model. Histological assessment was conducted to analyze the expressions levels of extracellular signal-regulated kinase (ERK), RAC-α serine/threonine protein kinase (AKT) and vascular endothelial growth factor (VEGF) in experimental tumors. Results of the present study demonstrated that MTA2 was overexpressed in NSCLC cells. The growth, migration and invasion of NSCLC cells were markedly inhibited by AbMTA2. In addition, it was observed that the ERK/AKT and VEGF signaling pathways were both upregulated in MTA2-overexpressing NSCLC cells, and downregulated following silencing of MTA2 activation. ERK and AKT phosphorylation levels were downregulated in NSCLC cells and tumors following MTA2 silencing. The in vivo study demonstrated that tumor growth was markedly inhibited following siRNA-MTA2 treatment. In conclusion, the results of the present study suggested that MTA2 silencing may significantly inhibit the growth and aggressiveness of NSCLC cells. Results from the present study indicated that the mechanism underlying the MTA2-mediated invasive potential of NSCLC cells involved the ERK/AKT and VEGF signaling pathways, which may be a potential therapeutic target for the treatment of NSCLC.

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Immunohistochemistry of deparaffinised sections using antigen retrieval with microwave combined pressure cooking versus immunofluorescence in the assessment of human renal biopsies

Abstract: IHC is superior to IF-F for immunopathological diagnosis of renal biopsy tissue and is a reliable replacement for the more traditional IF-F method.

Cited by 8 publications

References 31 publications

Hidden Tiger: An Atypical Presentation of Anti-Glomerular Basement Membrane Disease

Hidden Tiger: An Atypical Presentation of Anti-Glomerular Basement Membrane Disease

Diagnostic value of immunohistochemistry on formalin-fixed, paraffin-embedded skin biopsy specimens for bullous pemphigoid

Metastasis-associated protein 2 promotes the metastasis of non-small cell lung carcinoma by regulating the ERK/AKT and VEGF signaling pathways

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