Immunohistopathologic evaluation of Drynaria <i>fortunei</i> rhizome extract in the management of Class II furcation defects in a canine model

Afifi, Marwa M.; Kotry, Gehan S.; El-Kimary, Gillan; Youssef, Hayat A.

doi:10.1002/jper.17-0655

Cited by 5 publications

(12 citation statements)

References 33 publications

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“…An additional finding in the current histomorphometric analysis was a reduction in osteoblastic count in both experimental and control groups across the study period from first to second follow up intervals. It is quite clear that there is an indirect relationship between osteoblastic count and the degree of bone maturation over time (29). This finding is in accordance with another immunohistopathologic study conducted by Afifi et al (29) in a canine model with different periodontal defects.…”

Section: Discusionsupporting

confidence: 77%

“…It is quite clear that there is an indirect relationship between osteoblastic count and the degree of bone maturation over time (29). This finding is in accordance with another immunohistopathologic study conducted by Afifi et al (29) in a canine model with different periodontal defects. The authors evaluated the efficacy of guided tissue regeneration approach in managing furcation involvement defects.…”

Section: Discusionsupporting

confidence: 77%

“…Bone regeneration can be improved by incorporating the concept of tissue engineering to enhance the quantity and quality of the newly formed bone. Because of the limitations of some current osteoconductive bone grafting materials, alternative methods for enhancing osteogenic properties of these materials in repairing bone defects were deemed necessary (27)(28)(29).…”

Section: Discusionmentioning

confidence: 99%

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Histomorphometric Assessment of Autogenous Stem Cell Therapy in Horizontal Alveolar Ridge Augmentation (Experimental Study)

El-Kimary

Hommos

Sayed

et al. 2021

Alexandria Dental Journal

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INTRODUCTION:Augmentation of deficient alveolar ridge is a challenging task in periodontal regenerative therapy. The gold standard therapeutic modality for managing these defects is autogenous bone grafting. But, this approach was hampered by increased patient morbidity. Thus, recent advances in molecular and cellular biology have shed light on the ''bioengineering'' technology which seeks to introduce the incorporation of stem cells in periodontal regeneration. OBJECTIVE:The aim of this experimental study was to evaluate, histomorphometrically, the efficacy of guided bone regeneration (GBR) technique using autogenous bone marrow-derived mesenchymal stem cells (BMSCs) loaded on β-tricalcium phosphate (β-TCP) alloplast as a scaffold, in managing horizontal alveolar ridge defects. MATERIALS AND METHODS: Sixteen critical-sized horizontal ridge defects were created in four healthy mongrel dogs after bilateral extraction of the mandibular third and fourth premolars. In a split mouth design; the experimental group (right side): comprised eight ridge defects managed using GBR approach (autogenous BMSCs + β-TCP + collagen membrane). The control group (left side): comprised eight ridge defects were left empty to heal spontaneously. Dogs were sacrificed at 1 and 2 months intervals for histomorphometric assessment using Image J software to measure osteoblastic count (OC) from the obtained histological sections. RESULTS:The experimental group showed a statistically significant increase in OC at both follow up intervals of the study. CONCLUSIONS: GBR technique using autogenous BMSCs/ β-TCP composites was effective in managing horizontal ridge defects. Osteogenic properties and bone regenerative capacity of alloplasts can be greatly enhanced using this technique in periodontal regenerative surgeries.

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Section: Discusionsupporting

confidence: 77%

Section: Discusionsupporting

confidence: 77%

Section: Discusionmentioning

confidence: 99%

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Histomorphometric Assessment of Autogenous Stem Cell Therapy in Horizontal Alveolar Ridge Augmentation (Experimental Study)

El-Kimary

Hommos

Sayed

et al. 2021

Alexandria Dental Journal

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“…Dogs has been extensively used as animal models for medical research and drug evaluation, such as studying human breast cancer carcinogenesis ( Abdelmegeed and Mohammed, 2018 ), Duchenne muscular dystrophy (DMD) ( Wells, 2018 ), management of furcation defect ( Afifi et al, 2018 ), chronic myocardial infarction (MI) ( Xiong et al, 2018 ), comparative pathogenesis study of H3N2 canine influenza virus (CIV) challenged ( Luo et al, 2018 ), long term safety and efficacy of AAV gene therapy ( Lee et al, 2018 ). Therefore, it is critical to make sure that the dogs used as laboratory animals are free of special pathogens.…”

Section: Introductionmentioning

confidence: 99%

Multiplex Detection of Five Canine Viral Pathogens for Dogs as Laboratory Animals by the Luminex xTAG Assay

Feng

Zhu

et al. 2018

Front. Microbiol.

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More and more dogs have been used as a disease model for medical research and drug safety evaluation. Therefore, it is important to make sure that the dogs and their living houses are special pathogen free. In this study, the development and evaluation of a Luminex xTAG assay for simultaneous detection of five canine viruses was carried out, including canine distemper virus, canine parvovirus, canine parainfluenza virus, canine adenovirus, and rabies virus. Assay specificity was accomplished by targeting conserved genomic regions for each virus. Hybridization between multiplexed PCR products and the labeled fluorescence microspheres was detected in a high throughput format using a Luminex fluorescence reader. The Luminex xTAG assay showed high sensitivity with limits of detection for the five viruses was 100 copies/μL. Specificity of the xTAG assay showed no amplification of canine coronavirus, pseudorabies virus and canine influenza virus indicating that the xTAG assay was specific. Seventy-five clinical samples were tested to evaluate the xTAG assay. The results showed 100% coincidence with the conventional PCR method. This is the first report of a specific and sensitive multiplex Luminex xTAG assay for simultaneous detection of five major canine viral pathogens. This assay will be a useful tool for quality control and environmental monitoring for dogs used as laboratory animals, may even be applied in laboratory epidemiological investigations.

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“…A sulcular incision at the buccal aspect of the mandibular third (P 3 ) and fourth (P 4 ) premolars was made in the right and left quadrants. The mucoperiosteal flap was fully reflected, then a rotary 0.5 mm rounded carbide bur was used to create Grade-II critical-sized defects of 3 × 4 mm 2 and 3.5 × 4.5 mm 2 at furcation area in P3 and P4, respectively for each dog 19,22 . The inter-radicular bone was removed under copious irrigation using sterile saline.…”

mentioning

confidence: 99%

Histologic and histomorphometric evaluation of Aloe vera adjunctive to β-tricalcium phosphate in class II furcation defects in dogs

Taalab

Rehim

Eldeeb

et al. 2023

Sci Rep

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Aloe vera has antimicrobial activity and enhances the osseointegration process, thus it may have the potential in treating periodontal defects. This study aimed to evaluate the effect of Aloe vera as an adjunction to Beta-tricalcium phosphate (β-TCP) bone graft in Grade II furcation defects. A randomized study was conducted on six healthy mongrel dogs' premolars. A total of twenty-four Grade II furcation critical-sized defects were surgically created after reflecting a full-thickness flap, twelve defects were filled with β-TCP while the other twelve defects were filled with Aloe vera mixed with β-TCP and both covered by collagen membrane. Animals were euthanized at the end of the fourth and eighth week and defects were analyzed histologically and histomorphometrically. Histologically, Aloe vera mixed with β-TCP resulted in more bone formation and new PDL fibers compared to β-TCP alone. After 2 and 4 weeks, the experimental group had significantly higher newly formed interradicular bone height (p < 0.0001, and p < 0.0001, respectively), bone thickness (p < 0.0001, and p < 0.0001, respectively), and percentage of the surface area (p = 0.009, and p = 0.023, respectively). Aloe vera gel adjunctive to β-TCP is an effective bioactive agent that enhances periodontal tissue regeneration and bone formation in critically sized defects.

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Immunohistopathologic evaluation of Drynaria fortunei rhizome extract in the management of Class II furcation defects in a canine model

Abstract: Drynaria fortunei demonstrated increased regeneration and bone formation when used in the treatment of furcation defects in a canine model.

Cited by 5 publications

References 33 publications

Histomorphometric Assessment of Autogenous Stem Cell Therapy in Horizontal Alveolar Ridge Augmentation (Experimental Study)

Histomorphometric Assessment of Autogenous Stem Cell Therapy in Horizontal Alveolar Ridge Augmentation (Experimental Study)

Multiplex Detection of Five Canine Viral Pathogens for Dogs as Laboratory Animals by the Luminex xTAG Assay

Histologic and histomorphometric evaluation of Aloe vera adjunctive to β-tricalcium phosphate in class II furcation defects in dogs

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