Both Egyptian propolis and nanohydroxyapatite graft material showed favorable periodontal regenerative effect. Propolis showed increased cellular proliferative ability that could be beneficial in reducing the healing period needed after periodontal therapy.

INTRODUCTION: Nanotechnology has been greatly utilized for bone regeneration strategies. It helps overcome some of the current limitations associated with bone regeneration methods Propolis is a natural substance made by the honeybee, it has effective antioxidant, antimicrobial, and anti-inflammatory effects. Moreover, it has multiple biological effects including improvement of wound healing and bone regeneration. OBJECTIVES: comparison of the effectiveness of NanoBone graft and propolis material on the inter-radicular bone regeneration in Mongrel dogs. MATERIALS AND METHODS: A split mouth design was carried out using the third and fourth mandibular premolars of 3 healthy mongrel dogs. A total of 12 grade II furcation defects were surgically created. The defects in the right side were treated with NanoBone graft and then a collagen membrane was used to cover the defects. In the left side the defects were treated with propolis and then covered with collagen membrane. The dogs were sacrificed after 4 weeks. Segments of the jaw bone containing the defects were then dissected out and prepared for histological evaluation. RESULTS: Both percentage of bone fill and percentage of bone surface area in defects treated with propolis powder were higher than those in NanoBone-filled defects. CONCLUSIONS: Both propolis and NanoBone graft materials have bone regenerative effects with higher ability of propolis to regenerate bone.

Aloe vera has antimicrobial activity and enhances the osseointegration process, thus it may have the potential in treating periodontal defects. This study aimed to evaluate the effect of Aloe vera as an adjunction to Beta-tricalcium phosphate (β-TCP) bone graft in Grade II furcation defects. A randomized study was conducted on six healthy mongrel dogs' premolars. A total of twenty-four Grade II furcation critical-sized defects were surgically created after reflecting a full-thickness flap, twelve defects were filled with β-TCP while the other twelve defects were filled with Aloe vera mixed with β-TCP and both covered by collagen membrane. Animals were euthanized at the end of the fourth and eighth week and defects were analyzed histologically and histomorphometrically. Histologically, Aloe vera mixed with β-TCP resulted in more bone formation and new PDL fibers compared to β-TCP alone. After 2 and 4 weeks, the experimental group had significantly higher newly formed interradicular bone height (p < 0.0001, and p < 0.0001, respectively), bone thickness (p < 0.0001, and p < 0.0001, respectively), and percentage of the surface area (p = 0.009, and p = 0.023, respectively). Aloe vera gel adjunctive to β-TCP is an effective bioactive agent that enhances periodontal tissue regeneration and bone formation in critically sized defects.

INTRODUCTION: Alveolar ridge preservation strategies are indicated to minimize the loss of ridge volume that typically follows tooth extraction. Dentin and bone are mineralized tissues and almost similar in chemical components. Particulate dentin has a plenty of growth factors such as bone morphogenetic proteins (BMP) that can induce new bone formation. Platelets play a fundamental role in hemostasis and are a natural source of growth factors. Mixture of particulate dentin and platelet-rich plasma can have an osteoinductive effect during different stages of bone healing. OBJECTIVES: To investigate the biological effect of autogenous dentin graft combined with platelet rich plasma on alveolar bone healing following tooth extraction in rabbit. MATERIALS AND METHODS: Seven healthy male New Zealand rabbits weighing 3kg (±250 g) were included in this study. The upper right incisor of each rabbit was extracted .The root of each incisor was used as a source of dentin. Root dentin was ground into powder by means of mortar and pestle.2 ml blood were taken from each rabbit. Platelet rich plasma was separated from other blood components by centrifuge machine. The left and right lower first premolars were extracted at the same time .The sockets of the lower right first premolars were filled by the mixture of dentin and platelet rich plasma (study group) while sockets of the lower left premolars were left to heal spontaneously (control group). Comparison of the healing features between the two groups was made histologically and histomorhometrically after 6 weeks. RESULTS: New bone formation was noticed in the sockets loaded with dentin and platelet rich plasma mixture. The newly formed bone was significantly higher when compared to that in the control group. CONCLUSIONS combination of dentin and platelet rich plasma induces new bone formation.

Objective: Evaluating the effectiveness of Angelica sinensis, a widely used traditional Chinese medicine, in conjunction with Beta-tricalcium phosphate (β-TCP) bone graft in the management of induced critical sized class II furcation defects in adult healthy mongrel dogs. Materials and Methods: A randomized study design was conducted on the third and fourth premolars of six healthy mongrel dogs. A total of twenty-four class II furcation critical-sized defects were surgically created. After reflecting a fully-reflected mucoperiosteal flap, twelve defects were filled with Angelica sinensis powder mixed with Beta-tricalcium phosphate (β-TCP), then collagen membranes were applied over the defects (experimental group). The other twelve defects were filled with β-TCP followed by collagen membrane application (control group). The dogs were sacrificed at the end of fourth and eighth weeks. Jaw segments containing the operated teeth were dissected and processed for histological examination and morphometric analysis of the regenerative features. Results: The histological results revealed that bone formation and regeneration of cementum and PDL progressed more in experimental groups than in control groups. Moreover, the regenerative features were more advanced in the second observation period. The histomorphometry revealed that the surface area of newly formed bone and the thickness of its trabeculae were higher in the defects treated with Angelica sinensis and β-TCP than in only β-TCP-filled defects at both observational periods. Conclusions: Angelica sinensis has accelerated the regenerative potential of the interradicular bone and has enhanced the effect of β-TCP for interradicular bone regeneration in dogs.