Immunoinformatics analysis to design novel epitope based vaccine candidate targeting the glycoprotein and nucleoprotein of Lassa mammarenavirus (LASMV) using strains from Nigeria

Abass, Ohilebo Abdulateef; Тимофеев, В. И.; Sarkar, Bishajit; Onobun, Desmond Odiamehi; Ogunsola, Samuel Olarewaju; Aiyenuro, Ademola; Aborode, Abdullahi Tunde; Aigboje, Adebayo Emmanuel; Omobolanle, Basheer Nabilat; Imolele, Anthony Goodwill; Abiodun, Alade Adebowale

doi:10.1080/07391102.2021.1896387

Cited by 19 publications

(14 citation statements)

References 129 publications

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“…Mugunthan et al [72] study result was more similar to our observation which the MFE was reported as ΔG = −156.50 kcal/ mol (present study result~-163.70 kcal/mol). This finding is also consistent with similar studies [68][69][70][71][72]86] which totally proposed that present vaccine may have stable mRNA structure following entry, transcript and expression in host.…”

Section: Discussionsupporting

confidence: 92%

“…The secondary structure of the vaccine mRNA sequence was predicted by the RNAfold server with a MFE score of -163.70 kcal/mol. The prediction of the secondary structure of the multiepitope vaccine mRNA is shown in Fig 11. This finding is in consistent with similar studies https://doi.org/10.1371/journal.pone.0275237.g007 [68][69][70][71][72], which totally proposed that present vaccine may have stable mRNA structure following entry, transcript and expression in host.…”

Section: Mrna Prediction Of the Designed Vaccinesupporting

confidence: 91%

“…Many studies have shown that MBLs (IMP, VIM, and NDM) are present as soluble proteins in cells [12,[19][20][21]. Vaccines are an important tools in public health and play an important role in controlling infectious diseases, but the process of developing and producing them is costly and sometimes it takes years to get a suitable vaccine against a particular pathogen [69]. For this reason, scientists today are working on subunit vaccines using in silico approaches.…”

Section: Discussionmentioning

confidence: 99%

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In silico designing and immunoinformatics analysis of a novel peptide vaccine against metallo-beta-lactamase (VIM and IMP) variants

et al. 2023

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The rapid spread of acquired metallo-beta-lactamases (MBLs) among gram negative pathogens is becoming a global concern. Improper use of broad-spectrum antibiotics can trigger the colonization and spread of resistant strains which lead to increased mortality and significant economic loss. In the present study, diverse immunoinformatic approaches are applied to design a potential epitope-based vaccine against VIM and IMP MBLs. The amino acid sequences of VIM and IMP variants were retrieved from the GenBank database. ABCpred and BCPred online Web servers were used to analyze linear B cell epitopes, while IEDB was used to determine the dominant T cell epitopes. Sequence validation, allergenicity, toxicity and physiochemical analysis were performed using web servers. Seven sequences were identified for linear B cell dominant epitopes and 4 sequences were considered as dominant CD4+ T cell epitopes, and the predicted epitopes were joined by KK and GPGPG linkers. Stabilized multi-epitope protein structure was obtained using molecular dynamics simulation. Molecular docking showed that the designed vaccine exhibited sustainable and strong binding interactions with Toll-like receptor 4 (TLR4). Finally, codon adaptation and in silico cloning studies were performed to design an effective vaccine production strategy. Immune simulation significantly provided high levels of immunoglobulins, T helper cells, T-cytotoxic cells and INF-γ. Even though the introduced vaccine candidate demonstrates a very potent immunogenic potential, but wet-lab validation is required to further assessment of the effectiveness of this proposed vaccine candidate.

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Section: Discussionsupporting

confidence: 92%

Section: Mrna Prediction Of the Designed Vaccinesupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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In silico designing and immunoinformatics analysis of a novel peptide vaccine against metallo-beta-lactamase (VIM and IMP) variants

et al. 2023

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“…At present, multi-epitope-based peptide vaccine design and development is an emerging area of research that focuses on using specific components of a pathogen, known as epitopes, to create a vaccine (Abass et al 2022 ). Epitopes are short amino acid sequences recognized by the immune system and trigger an immune response.…”

Section: Introductionmentioning

confidence: 99%

“…These robust and more sophisticated algorithms are the hub for identifying immune epitopes against T and B cells. Various high-throughput screening approaches have already been developed to evaluate a vaccine construct’s efficacy (Abass et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Advances in Computational and Bioinformatics Tools and Databases for Designing and Developing a Multi-Epitope-Based Peptide Vaccine

Shawan,

Sharma,

Halder

et al. 2023

Int J Pept Res Ther

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A vaccine is defined as a biologic preparation that trains the immune system, boosts immunity, and protects against a deadly microbial infection. They have been used for centuries to combat a variety of contagious illnesses by means of subsiding the disease burden as well as eradicating the disease. Since infectious disease pandemics are a recurring global threat, vaccination has emerged as one of the most promising tools to save millions of lives and reduce infection rates. The World Health Organization reports that immunization protects three million individuals annually. Currently, multi-epitope-based peptide vaccines are a unique concept in vaccine formulation. Epitope-based peptide vaccines utilize small fragments of proteins or peptides (parts of the pathogen), called epitopes, that trigger an adequate immune response against a particular pathogen. However, conventional vaccine designing and development techniques are too cumbersome, expensive, and time-consuming. With the recent advancement in bioinformatics, immunoinformatics, and vaccinomics discipline, vaccine science has entered a new era accompanying a modern, impressive, and more realistic paradigm in designing and developing next-generation strong immunogens. In silico designing and developing a safe and novel vaccine construct involves knowledge of reverse vaccinology, various vaccine databases, and high throughput techniques. The computational tools and techniques directly associated with vaccine research are extremely effective, economical, precise, robust, and safe for human use. Many vaccine candidates have entered clinical trials instantly and are available prior to schedule. In light of this, the present article provides researchers with up-to-date information on various approaches, protocols, and databases regarding the computational designing and development of potent multi-epitope-based peptide vaccines that can assist researchers in tailoring vaccines more rapidly and cost-effectively.

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Immunoinformatics and Vaccine Development

Shukla,

Mani

2024

Unraveling New Frontiers and Advances in Bioinformatics

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Immunoinformatics analysis to design novel epitope based vaccine candidate targeting the glycoprotein and nucleoprotein of Lassa mammarenavirus (LASMV) using strains from Nigeria

Cited by 19 publications

References 129 publications

In silico designing and immunoinformatics analysis of a novel peptide vaccine against metallo-beta-lactamase (VIM and IMP) variants

In silico designing and immunoinformatics analysis of a novel peptide vaccine against metallo-beta-lactamase (VIM and IMP) variants

Advances in Computational and Bioinformatics Tools and Databases for Designing and Developing a Multi-Epitope-Based Peptide Vaccine

Immunoinformatics and Vaccine Development

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