Immunoinformatics guided modeling of CCHF_GN728, an mRNA-based universal vaccine against Crimean-Congo hemorrhagic fever virus

Shahrear, Sazzad; Islam, Abul B.M.M.K.

doi:10.1016/j.compbiomed.2021.105098

Cited by 8 publications

(5 citation statements)

References 88 publications

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“…Immunoinformatic approaches were used in a study to develop a novel mRNA vaccine that is safe, engineered and efficient. This mRNA-based vaccine has been shown to be effective against various viral infections such as Zika, influenza, rabies, coronavirus and many others [ 31 ]. Recently, multiple human clinical trials have begun, indicating that mRNA vaccines are now considered to be a safe and effective alternative to subunit protein, chimeric virus and even DNA-based therapies in the form of vaccination [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Development of innovative multi-epitope mRNA vaccine against Pseudomonas aeruginosa using in silico approaches

Asadinezhad,

Khoshnood,

Asadollahi

et al. 2023

Briefings in Bioinformatics

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The rising issue of antibiotic resistance has made treating Pseudomonas aeruginosa infections increasingly challenging. Therefore, vaccines have emerged as a viable alternative to antibiotics for preventing P. aeruginosa infections in susceptible individuals. With its superior accuracy, high efficiency in stimulating cellular and humoral immune responses, and low cost, mRNA vaccine technology is quickly replacing traditional methods. This study aimed to design a novel mRNA vaccine by using in silico approaches against P. aeruginosa. The research team identified five surface and antigenic proteins and selected their appropriate epitopes with immunoinformatic tools. These epitopes were then examined for toxicity, allergenicity and homology. The researchers also checked their presentation and identification by major histocompatibility complex cells and other immune cells through valuable tools like molecular docking. They subsequently modeled a multi-epitope protein and optimized it. The mRNA was analyzed in terms of structure and stability, after which the immune system’s response against the new vaccine was simulated. The results indicated that the designed mRNA construct could be an effective and promising vaccine that requires laboratory and clinical trials.

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Section: Discussionmentioning

confidence: 99%

Development of innovative multi-epitope mRNA vaccine against Pseudomonas aeruginosa using in silico approaches

Asadinezhad,

Khoshnood,

Asadollahi

et al. 2023

Briefings in Bioinformatics

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“…The researchers’ results indicate that the CCHF_GN728 vaccine will elicit targeted immune responses against the CCHFV virus. Their model is prepared for wet lab experiments to evaluate the effectiveness of this potential vaccination candidate [ 203 ].…”

Section: Vaccination Methods Against Cchfvmentioning

confidence: 99%

“…The researchers’ results indicate that the CCHF_GN728 vaccine will elicit targeted immune responses against the CCHFV virus. [ 203 ] CCHFV-MAfg09 vaccine DNA vaccine Demonstrates that the protective effectiveness of the M-segment-based DNA vaccine against CCHFV depends on the CD8 + T-cell response. Since antigenic strategies to target CD8 + T cells differ from a global immune system strategy, the insights garnered from these studies could be utilized to enhance the design of the GPC component of CCHFV vaccines.…”

Section: Vaccination Methods Against Cchfvmentioning

confidence: 99%

Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges

Muzammil,

Rayyani,

Abbas Sahib

et al. 2024

Biol Proced Online

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Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus, and zoonosis, and affects large regions of Asia, Southwestern and Southeastern Europe, and Africa. CCHFV can produce symptoms, including no specific clinical symptoms, mild to severe clinical symptoms, or deadly infections. Virus isolation attempts, antigen-capture enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) are all possible diagnostic tests for CCHFV. Furthermore, an efficient, quick, and cheap technology, including biosensors, must be designed and developed to detect CCHFV. The goal of this article is to offer an overview of modern laboratory tests available as well as other innovative detection methods such as biosensors for CCHFV, as well as the benefits and limits of the assays. Furthermore, confirmed cases of CCHF are managed with symptomatic assistance and general supportive care. This study examined the various treatment modalities, as well as their respective limitations and developments, including immunotherapy and antivirals. Recent biotechnology advancements and the availability of suitable animal models have accelerated the development of CCHF vaccines by a substantial margin. We examined a range of potential vaccines for CCHF in this research, comprising nucleic acid, viral particles, inactivated, and multi-epitope vaccines, as well as the present obstacles and developments in this field. Thus, the purpose of this review is to present a comprehensive summary of the endeavors dedicated to advancing various diagnostic, therapeutic, and preventive strategies for CCHF infection in anticipation of forthcoming hazards. Graphical Abstract

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“…New vaccine delivery systems are being developed to protect against ticks and tick-borne diseases, including the use of lipid nanoparticle (LNP)-mRNA vaccines [ 121 , 122 ]. This technology offers an alternative to live attenuated or subunit-based vaccines [ 123 ]. An Ixodes scapularis salivary gland protein encoded by mRNA using this system decreased tick engorgement and B. burgdorferi transmission [ 122 ].…”

Section: Advances and Remaining Challenges To Universal Tick Vaccinesmentioning

confidence: 99%

Universal Tick Vaccines: Candidates and Remaining Challenges

Parizi

Githaka

Logullo

et al. 2023

Animals

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Recent advancements in molecular biology, particularly regarding massively parallel sequencing technologies, have enabled scientists to gain more insight into the physiology of ticks. While there has been progress in identifying tick proteins and the pathways they are involved in, the specificities of tick-host interaction at the molecular level are not yet fully understood. Indeed, the development of effective commercial tick vaccines has been slower than expected. While omics studies have pointed to some potential vaccine immunogens, selecting suitable antigens for a multi-antigenic vaccine is very complex due to the participation of redundant molecules in biological pathways. The expansion of ticks and their pathogens into new territories and exposure to new hosts makes it necessary to evaluate vaccine efficacy in unusual and non-domestic host species. This situation makes ticks and tick-borne diseases an increasing threat to animal and human health globally, demanding an urgent availability of vaccines against multiple tick species and their pathogens. This review discusses the challenges and advancements in the search for universal tick vaccines, including promising new antigen candidates, and indicates future directions in this crucial research field.

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Immunoinformatics guided modeling of CCHF_GN728, an mRNA-based universal vaccine against Crimean-Congo hemorrhagic fever virus

Cited by 8 publications

References 88 publications

Development of innovative multi-epitope mRNA vaccine against Pseudomonas aeruginosa using in silico approaches

Development of innovative multi-epitope mRNA vaccine against Pseudomonas aeruginosa using in silico approaches

Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges

Universal Tick Vaccines: Candidates and Remaining Challenges

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