Immunolocalization of Gastrin-Releasing Peptide in the Bovine Uterus and Placenta.

Budipitojo, Teguh; Matsuzaki, Shinya; Cruzana, Maria Bella C.; Baltazar, E. T.; Hondo, Eiichi; Sunaryo, Sumiyati; Kitamura, Nobuo; Yamada, Junzo

doi:10.1292/jvms.63.11

Cited by 15 publications

(14 citation statements)

References 20 publications

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“…The abundance of the gastrin-releasing peptide (GRP) mRNA was 4-fold higher at oestrus than at dioestrus. GRP belongs to a group of regulatory peptides and was found to be expressed in the reproductive tract of different species (Happola & Lakomy 1989, Fraser et al 1994, Whitley et al 1996, Budipitoj et al 2001. In sheep a study of GRP mRNA expression during the oestrous cycle in the uterus showed that expression was markedly increased at day 16 compared with days 4, 10, 12 and 14 (Whitley et al 1998); day 0 was not investigated.…”

Section: Discussionmentioning

confidence: 99%

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Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

Bauersachs¹,

Rehfeld²,

Ulbrich³

et al. 2004

Journal of Molecular Endocrinology

117

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The oviduct epithelium undergoes marked morphological and functional changes during the oestrous cycle. To study these changes at the level of the transcriptome we did a systematic gene expression analysis of bovine oviduct epithelial cells at oestrus and dioestrus using a combination of subtracted cDNA libraries and cDNA array hybridisation. A total of 3072 cDNA clones of two subtracted libraries were analysed by array hybridisation with cDNA probes derived from six cyclic heifers, three of them slaughtered at oestrus and three at dioestrus. Sequencing of cDNAs showing significant differences in their expression levels revealed 77 different cDNAs. Thirty-seven were expressed at a higher level at oestrus, for the other 40 genes expression levels were higher at dioestrus. The identified genes represented a variety of functional classes. During oestrus especially genes involved in the regulation of protein secretion and protein modification, and mRNAs of secreted proteins, were up-regulated, whereas during dioestrus particularly transcripts of genes involved in transcription regulation showed a slight up-regulation. The concentrations of seven selected transcripts were quantified by real-time RT-PCR to validate the cDNA array hybridisation data. For all seven transcripts, RT-PCR results were in excellent correlation (r>0·92) with the results obtained by array hybridisation. Our study is the first to analyse changes in gene expression profiles of bovine oviduct epithelial cells during different stages of the oestrous cycle, providing a starting point for the clarification of the key transcriptome changes in these cells.

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Section: Discussionmentioning

confidence: 99%

“…In sheep a study of GRP mRNA expression during the oestrous cycle in the uterus showed that expression was markedly increased at day 16 compared with days 4, 10, 12 and 14 (Whitley et al 1998); day 0 was not investigated. The expression in the uterus was located mainly to the uterine gland epithelial cells (Budipitoj et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

Bauersachs¹,

Rehfeld²,

Ulbrich³

et al. 2004

Journal of Molecular Endocrinology

117

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“…Sections were examined with a c o n v e n t i o n a l l i g h t m i c r o s c o p e , a n d photomicrographs were taken with a digital camera. * Crown-rump-length of fetus, Intensity of immunoreactivities: -, not detected; ±, very weak; +, weak; ++, moderate, The strong intensity (+++) of GRP immunoreactivity was deduced from the previous report of GRP immunolocalization in the uterine gland of pregnant cow [29].…”

Section: In Situ Hybridizationmentioning

confidence: 90%

“…G R P immunoreactivity has also been detected in the superficial epithelial cells of the intercotyledonary endometrium of sheep [26], the trophoblast, t ro p ho bl as ti c gian t c ell s, h ybr id c ell s and superficial epithelial cells of the caruncle of the cow [29]. Furthermore, since GRP mRNA is expressed only at day 16 in the estrous cycle of sheep, it was suggested that the appearance of GRP in uterine gland cells depends on the stage of the estrous cycle [31].…”

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confidence: 98%

“…R a d i o i m m u n o a s s a y a n d immunohistochemical analysis revealed that GRPlike immunoreactivity was distributed in various tissues from a variety of mammalian species. These include gastrointestinal tissues [17], brain [18], pituitary gland [19], spinal cord [20], sensory and sympathetic ganglia [21,22], adrenal gland [23], lung [24], thyroid [25] and genitourinary tract [26][27][28][29]. Moreover, some neuroendocrine tumors express GRP-like immunoreactivity, such as small cell lung carcinomas and medullary thyroid carcinomas [24,30].…”

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confidence: 99%

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Localization and Expression of Gastrin-releasing Peptide (GRP) in the Bovine Cervix

Budipitojo

Sasaki

Matsuzaki³

et al. 2004

Journal of Reproduction and Development

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Abstract. Gastrin-releasing peptide (GRP) has been suggested as a novel regulatory peptide in the female reproductive tract but the presence of GRP and GRP mRNA in the non-neurogenic tissue of the cervix has not yet been clarified. In the present study, immunohistochemistry and in situ hybridization were used to reveal the distribution of GRP immunoreactivity and expression of GRP mRNA in the bovine cervix. The cervixes from 21 non-pregnant and 20 pregnant cows, and 6 fetuses were used in the study. In the fetus, adult non-pregnant and pregnant specimens, GRP and GRP mRNA were predominantly detected in the luminal epithelial cells of basal areas of peripheral regions of the cervix. Positive staining of GRP in the epithelial cells of the cervix was first detected in the CRL 37 cm of the fetus. During the estrous cycles, the staining intensity of GRP in the epithelial cells was stronger in the follicular phase than in the luteal phase. During the early gestational period, GRP immunoreactivity was detected at relatively similar intensity to the follicular phase. In situ hybridization results ascertained the expression of GRP mRNA in the superficial epithelial cells of the cervix of non-pregnant and pregnant cows. The results suggest that GRP may be important both in the development of the fetal cervix and secretory activity of the epithelial cells of the cervix. Key words: Cervix, Gastrin-releasing peptide (GRP), Immunohistochemistry, In situ hybridization (J. Reprod. Dev. 50: [119][120][121][122][123][124][125][126][127][128][129] 2004) astrin-releasing peptide (GRP) is a member of the bombesin (BN)-like peptide family in mammals. A novel active polypeptide, BN was found for the first time in the skin of the European amphibian, Bombina bombina [1]. Other peptides related to BN were purified from amphibians, and classified into two other families, ranatensin and phyllolitorin families [2][3][4]. Among these three BNlike peptide families, mammalian homologues were found only fo r the BN and ranatensin families, but not for the phyllolitorin family. In 1979, a mammalian counterpart for BN was isolated from porcine gastric and intestinal tissues. This 27-amino acid peptide was the first BN-related peptide found in mammals, and was called GRP according to its activity originally found [5]. GRP regulates numerous gastrointestinal functions, such as the r e l e as e o f g a st r oi n t e s t i n a l h o r m o n e s [ 6 ] , stimulation of hormone secretion from the pancreas

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Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray

Ishiwata

Katsuma

Kizaki

et al. 2003

Molecular Reproduction Devel

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Gene expression analysis comparing nonpregnant with pregnant bovine uteri, including placenta, was performed with a custom cDNA microarray containing 1,933 independent genes. These genes were classified into six categories according to biological function, as follows: cell and tissue structural dynamics (108 genes), intercellular communication (221), intracellular metabolism (265), cell cycle and apoptosis (26), regulation of gene expression (113), expressed sequence tag (EST) and function unknown (617), and uncomplemented genes (583 clones). This array possessed bovine placental/endometrial specificity, as it included many pregnancy-specific molecules, such as pregnancy-associated glycoprotein-1 (PAGs), placental lactogen (PLs), and prolactin-related protein-1 (PRPs). A total of 77 genes were induced and 12 repressed in the placenta/endometrium. Our results point to a fundamental role for bovine placental-specific genes such as PAGs, PLs, and PRPs, in implantation and placentogenesis, and document that cDNA microarray analysis from bovine placenta/endometrium is possible and is a specific tool for monitoring genome-wide gene expression during the establishment and maintenance of pregnancy.

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Immunolocalization of Gastrin-Releasing Peptide in the Bovine Uterus and Placenta.

Cited by 15 publications

References 20 publications

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

Monitoring gene expression changes in bovine oviduct epithelial cells during the oestrous cycle

Localization and Expression of Gastrin-releasing Peptide (GRP) in the Bovine Cervix

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray

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