Immunolocalization of NGF and Its Receptors trkA and p75 in the Oviducts of Golden Hamsters during the Estrous Cycle

Wang, Qiang; Shi, Zhanquan; Watanabe, Gen; Taya, Kazuyoshi

doi:10.1538/expanim.58.543

Cited by 16 publications

(18 citation statements)

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“…Studies in golden hamsters have shown that NGF and its two receptors trkA and p75 are present in the ovary and uterus, demonstrating that NGF and its receptors trkA and p75 have important autocrine and paracrine regulatory roles in ovarian and oviductal function and uterine growth and proliferation during the estrous cycle [21,[32][33][34]. In our previous studies, NGF and its two receptors trkA and p75 were observed in numerous interstitial and luteal cells of golden hamsters [32].…”

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confidence: 75%

“…Inhibin production is restricted mainly to steroidogenic tissues and the hypophysis, whereas activins are produced in many cell types and organs [24][25][26]. It is now clear that inhibin/activin subunits could exert local autocrine and paracrine roles on steroidogenesis and folliculogenesis within developing ovaries [27][28][29][30][31].Studies in golden hamsters have shown that NGF and its two receptors trkA and p75 are present in the ovary and uterus, demonstrating that NGF and its receptors trkA and p75 have important autocrine and paracrine regulatory roles in ovarian and oviductal function and uterine growth and proliferation during the estrous cycle [21,[32][33][34]. In our previous studies, NGF and its two receptors trkA and p75 were observed in numerous interstitial and luteal cells of golden hamsters [32].…”

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confidence: 99%

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Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

et al. 2009

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Abstract. The aim of this study was to investigate the effect of luteinizing hormone (LH) surge on expression of nerve growth factor (NGF), trkA, p75 and inhibin α-subunit in ovarian interstitial cells of golden hamsters after human chorionic gonadotropin (hCG) treatment. NGF, two NGF receptors (trkA, p75) and inhibin α-subunit were immunolocalized by immunohistochemistry, and gonadotropins, steroid hormones and immunoreactive (ir-) inhibin concentrations were measured by radioimmunoassay. Stronger positive staining of NGF, trkA and p75 were found in interstitial cells at 6, 12 and 18 h after hCG injection in the treated group compared with the control groups. Inhibin α-subunit staining was found in interstitial cells at 12 and 18 h after hCG injection in the treatment group, but not in the control group. Plasma concentrations of progesterone increased significantly from 6 to 18 h after hCG treatment, whereas plasma concentrations of estradiol-17β significantly decreased compared with the control group. An increased plasma concentration of FSH and decreased concentrations of ir-inhibin from 6 to 18 h after hCG treatment verified the negative relationship. There were no significant changes in the concentrations of LH in the hCG-treated group. In contrast, the typical preovulatory LH surge was found at 1700 h on day 4 (proestrus) in the control group. These results suggested that LH surge can induce expression of NGF, trkA, p75 and inhibin α-subunit in ovarian interstitial cells and that NGF, trkA, p75 and inhibin α-subunit may have a paracrine or autocrine role in modulation of ovarian interstitial cell function in golden hamsters. Key words: Golden hamster, Inhibin α-subunit, Interstitial cell, Nerve growth factor (NGF), p75 and trkA (J. Reprod. Dev. 55: [622][623][624][625][626][627][628] 2009) erve growth factor (NGF) belongs to a family of related proteins required for survival, maintenance and development of discrete neuronal populations in the central and peripheral nervous systems [1][2][3]. The effect of NGF has been shown to be mediated through a specific high-affinity membrane receptor, tyrosine kinase A (trkA), that is responsible for NGF's biological activities [4,5]. Furthermore, the effect of NGF is also mediated via low affinity p75 receptor, which is also a receptor of other neurotropins [6]. It is now well known that the mammalian ovary not only contains four of the known neurotropins [7][8][9][10][11][12], but also expresses the receptors for each of them [13,14]. Previous papers have indicated that NGF and its receptors play a critical role in the development of mammalian ovary, oogenesis and folliculogenesis [15][16][17][18][19][20][21]. Inhibin and activins are dimeric glycoproteins formed by two of three different subunits (α, βA and βB). Inhibins consist of either of the β-subunits dimerized with a common α-subunit (α:βA and α:βB; inhibin A and inhibin B, respectively). Activins are dimers of β-subunits (βA:βA, βA:βB and βB:βB; activin A, activin AB and activin B, respectively). Inhibin/...

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confidence: 75%

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confidence: 99%

Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

et al. 2009

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“…Another possible mechanism would involve the production of embryotropic factors, such as certain growth factors, whose presence in the oviduct has been clearly documented. Growth factors in the oviduct were suggested to be involved in preimplantation embryo development (Dalton et al 1994, Einspanier et al 1999, Pushpakumara et al 2002, Wijayagunawardane et al 2005, Itoh et al 2006, Sun et al 2006, Kawamura et al 2007, Weng et al 2009, Swangchan-Uthai et al 2011.…”

Section: R7mentioning

confidence: 99%

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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“…The presence of NTs in the surface and ductal epithelium could play a role in oviductal transport, fertilization, capacitation of spermatozoa and early embryonic development, as documented in some mammalian species (Lommatzsch et al, 1999;Ren et al, 2005;Shi et al, 2006;Weng et al, 2009;Li et al, 2012). Moreover, only NGF, taking into account its presence in glands of magnum, isthmus and uterus, might have an influence on the secretion of egg components.…”

Section: Tractsmentioning

confidence: 98%

“…To our knowledge, there are no data on the presence of NTs and Trks in the avian genital tract. Previous studies have been carried out in mammalian species, documenting the involvement in the oocyte transport and fertilization, in spermatozoa capacitation (Weng et al, 2009), and in development of preimplantation embryos (Kawamura et al, 2007;Mirshokraei et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Neurotrophins and specific receptors in the oviduct tracts of Japanese quail (Coturnix coturnix japonica)

Maruccio¹,

Castaldo²,

D’Angelo³

et al. 2017

Annals of Anatomy - Anatomischer Anzeiger

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Immunolocalization of NGF and Its Receptors trkA and p75 in the Oviducts of Golden Hamsters during the Estrous Cycle

Cited by 16 publications

References 31 publications

Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

Immunodetection of NGF, trkA, p75 and Inhibin .ALPHA.-Subunit in Interstitial Cells of Golden Hamsters Treated with hCG

Oviductal secretion and gamete interaction

WITHDRAWN: Neurotrophins and specific receptors in the oviduct tracts of Japanese quail (Coturnix coturnix japonica)

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