Immunolocalization of the human basal epithelial marker monoclonal antibody 312C8-1 in normal tissue and mammary tumours of rodents

Tsubura, Airo; Inui, Toshihiko; Senzaki, Hideto; Morii, Sotokichi; Dairkee, Shanaz H.

doi:10.1007/bf00718646

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…This antibody stains myoepithelial cells of the mammary gland and basal cells of the epidermis in mice [10]. Anti-type IV collagen antiserum was produced by immunizing rabbits with type IV collagen purified from mouse kidneys [15].…”

Section: Antibody and Antiseramentioning

confidence: 99%

“…Single neoplasms contain a number of genotypically and phenotypically distinct populations of the tumor cells [8], and keratin is a useful marker for the classification of cell phenotype [9,10]. The remarkable correlation between metastasis and secretion of type IV collagenolytic protease suggests that type IV collagen, a component of the basement membrane protein, may play a special role in the metastatic process [11].…”

Section: Introductionmentioning

confidence: 99%

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Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors

Tsubura

Inui

Morii

et al. 1991

Breast Cancer Res Tr

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A transplantable pregnancy-dependent mouse mammary tumor, TPDMT-4, and its ovarian-dependent (T4-OR26) and autonomous (T4-OI96, T4-OI145, T4-OI165, T4-OI320 and T4-OI320CY) sublines were examined immunohistochemically for the expression of keratin 14 and type IV collagen. T4-OI96, T4-OI145, and T4-OI165, but not T4-OR26, T4-OI320, or T4-OI320CY, formed lung colonies (metastasis) after intravenous injection as a single-cell suspension. Despite the similar morphology of TPDMT-4 and its six sublines, only TPDMT-4 and the nonmetastatic sublines revealed a basal cell phenotype as defined by keratin 14 expression. Staining for type IV collagen was complete at the peripheries of the glandular structures in TPDMT-4 and nonmetastatic sublines but was patchy in the metastatic tumors.

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Section: Antibody and Antiseramentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors

Tsubura

Inui

Morii

et al. 1991

Breast Cancer Res Tr

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“…Moreover, loss of keratin 14-positive cells in transplantable sublines of mouse mammary carcinoma has been recently reported by the authors to be accompanied by the acquisition of lung-colonizing capability and patchy type IV collagen surrounding cancer nests (10). Keratin 14 has been thought to be identified only in cryostat sections (3,11,17), but it can now be seen in methacarn-fixed, dewaxed and actinasepretreated sections. In this paper, correlations among the immunolocalizations of some cytokeratins, muscle actin and type IV collagen are investigated in serial sec- Table 2.…”

mentioning

confidence: 95%

“…This antibody was produced against a 51 KD keratin isolated from a mammary carcinoma (presumably keratin 14, calculated at a 50 KD molecular weight). Other monoclonal antibodies, KA-1, LL002, RCK107, RKH4, and LL025, can be used for the identification of basal cells (6,8,11,12,16,17) in human breast and rodent mammary tissues, but most of these antibodies have been available only in cryostat sections. In this study, useful advance has been provided by the proper digestion of methacarnfixed sections with actinase before applying the first antibody.…”

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Immunohistochemical detection of keratin, actin and type IV collagen in serial sections of methacarn-fixed breast cancer tissues.

Senzaki

Tsubura

Shoji

et al. 1992

Acta Histochem. Cytochem

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Correlationsamong the immunolocalizations of keratin, actin and type IV collagen were investigated in serial sections of methacarn-fixed, paraffin-embedded mastectomy specimens taken from infiltrating breast cancer patients.Stainings of the filaments in pretreated sections with actinase were often weaker and/or less apparent in cancer cells than in normal mammary gland cells. If at least 30% of living cancer cells were stained, the case was scored positive.Positivity for 312C8-1 specific for basal cell type keratin 14 was observed in 8 of 60 cases, while for NCL-5D3 specific for luminal cell type keratins, positivity was observed in 57 of 60 cases. Five cases positive for HHF35 specific for muscle actin were also positive for 312C8-1 and NCL-5D3.Type IV collagen in cancer foci was patchy and/or absent, but small cancer nests surrounded completely by continuous type IV collagen, termed "in situ carcinoma components" were found in 25 of 60 cases.In all the components, stainings with 312C8-1 and/or NCL5D3 were found in some cancer cells, and the former was almost always seen in a basal orientation within the components. In conclusion, keratin 14-positive basal cells could be identified in paraffin sections, and these findings suggest that the basal cells are closely related to continuous type IV collagen and a progressive loss of the cells may promote metastasis through fragmented type IV collagen.

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Histopathology of myoepithelial (basocellular) hyperplasias in adenosis and epitheliosis of the breast demonstrated by the reactivity of cytokeratins and S100 protein

Bässler

Katzer

1992

Vichows Archiv A Pathol Anat

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This study on the different types of epithelial hyperplasia in fibrocystic disease was inspired by the observation of myoepithelial (basocellular) hyperplasia identified by strong expression of S100 protein and a weak reaction with antibodies against cytokeratin (KL1) in cells forming solid and acinar buds. The cells do not contain immunohistochemically detectable actin or desmin. Glandular transformation and proliferation give rise to basocellular circumductal adenosis. Normal breast tissue, 51 cases of fibrocystic disease with mild, florid and atypical hyperplasias, 7 fibroadenomas and 20 cases of carcinoma in situ were studied and a semiquantitative analysis revealed basal buds and adenosis in less than 40% of cases of mild hyperplasia and up to 73% in florid hyperplasia. Epitheliosis is characterized by a heterogeneous cell pattern with cells positive for S100 protein in 30-60%, but in small ducts up to 100% with an immediate connection to the basal cell layer were positive. Carcinoma in situ contained very rare tumour cells positive for S100 protein. The cells expressing S100 protein in terminal ducts, in adenosis and epitheliosis showed only some of the characteristics of myoepithelial cells, since they lack immunoreactivity with antibodies against actin. These basal clear cells are interpreted as transitional or indeterminate cells with features of myoepithelial precursor cells, but with the ability to develop basocellular nodular and glandular hyperplasia in the ductulo-lobular units in cases of adenosis and juvenile fibroadenoma.

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Immunolocalization of the human basal epithelial marker monoclonal antibody 312C8-1 in normal tissue and mammary tumours of rodents

Cited by 10 publications

References 22 publications

Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors

Loss of basal cell phenotype with acquisition of lung-colonizing capability in mouse mammary tumors

Immunohistochemical detection of keratin, actin and type IV collagen in serial sections of methacarn-fixed breast cancer tissues.

Histopathology of myoepithelial (basocellular) hyperplasias in adenosis and epitheliosis of the breast demonstrated by the reactivity of cytokeratins and S100 protein

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