Immunolocalization of transforming growth factor-a and its receptor in the normal and hyperoxia-exposed neonatal rat retina

Powers, Michael R.; Planck, Stephen R.

doi:10.1076/ceyr.16.3.177.15406

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…Since light damage is frequently used as a method to enable efficient culture of M€ uller glia, it is possible that the up-regulation of EGFR, triggered by light damage, contributes to this phenomenon. A study by Powers and Planck (1997) also reported EGFR expression in M€ uller glia in the developing rat retina. However, there was no quantitation in their study, and the localization to Muller glia was not demonstrated with co-labeling for M€ uller glial-specific markers.…”

Section: Discussionmentioning

confidence: 86%

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Liu

Gümüşçü

et al. 2006

Glia

111

121

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Epidermal growth factor (EGF) is known to promote proliferation of both retinal progenitors and Muller glia in vitro, but several questions remain concerning an in vivo role for this factor. In this study, we investigated whether the EGF receptor (EGFR) is necessary for the maintenance of normal levels of progenitor and Muller glial proliferation in vivo. Here, we show that (1) mice with homozygous deletion of the Egfr gene have reduced proliferation in late stages of retinal histogenesis, (2) EGF is mitogenic for M€ uller glia in vivo during the first two postnatal weeks in the rodent retina, (3) the effectiveness of EGF as a M€ uller glial mitogen declines in parallel with the decline in EGFR expression as the retina matures, and (4) following damage to the retina from continuous light exposure, EGFR expression is upregulated in M€ uller glia to levels close to those in the neonatal retina, resulting in a renewed mitotic response to EGF. Together with previous results from other studies, these data indicate that the downregulation of a growth factor receptor is one mechanism by which glial cells maintain mitotic quiescence in the mature nervous system. V V C 2006 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 86%

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Liu

Gümüşçü

et al. 2006

Glia

111

121

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“…In these studies, EGF was never shown to induce cell degeneration. Although EGF and its receptors are highly expressed in the rat retina during the early postnatal period (Anchan et al, 1991;Powers and Planck, 1997), including in PRs at postnatal day 5 (data not shown), nothing has been reported on EGF effects on postnatal retinal cells in culture. Here, activation of EGFR in PRs was observed clearly by phosphotyrosine immunodetection, providing evidence that EGF promotes postmitotic PR degeneration in vitro.…”

Section: Discussionmentioning

confidence: 96%

Survival of Purified Rat PhotoreceptorsIn VitroIs Stimulated Directly by Fibroblast Growth Factor-2

Fontaine¹,

Kinkl²,

Sahel³

et al. 1998

J. Neurosci.

104

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Basic fibroblast growth factor (FGF-2) influences the differentiation and survival of retinal photoreceptors in vivo and in vitro, but it is not known whether it acts directly on photoreceptor FGF receptors or indirectly through activation of surrounding cells. To clarify the effects of FGF-2 on photoreceptor survival, we developed a purified photoreceptor culture system. The outer nuclear layers of postnatal day 5-15 rat retinas were isolated by vibratome sectioning, and the photoreceptor fractions obtained were enzymatically dissociated. Photoreceptors were maintained in monolayer culture for 1 week in a chemically defined medium. Immunocytochemical labeling showed that >99.5% of cells were photoreceptors, and glial contamination represented approximately 0. 2%. Photoreceptors from postnatal day 5-9 retinas survived for at least 24 hr in vitro, whereas cells from postnatal day 10-15 retinas died rapidly. Subsequent studies performed with postnatal day 5 photoreceptors showed that their survival was increased in a dose-dependent manner after the addition of FGF-2. In control cultures, 36% of originally seeded photoreceptors were alive after 5 d in vitro, and in the presence of 20 ng/ml FGF-2 this number was doubled to 62%. This increase was not caused by proliferation of photoreceptor precursors. Denaturing or blocking FGF-2 prevented enhancement of survival. Conversely, only 25.5% of photoreceptors survived in the presence of epidermal growth factor (EGF). FGF- and EGF-receptor mRNA and proteins were detected in purified photoreceptors in vitro, and addition of FGF-2 or EGF led to tyrosine phosphorylation of photoreceptor proteins. These data support a direct mechanism of action for FGF-2 stimulation of photoreceptor survival.

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“…TGF-a was reportedly found in the normal and affected tissues of the eye, mainly in the retina [10,31,32,35,39]. TGF-a may be involved in the proliferation and differentiation of lens epithelial cells under physiological conditions and after cataract surgery.…”

Section: Discussionmentioning

confidence: 99%

Expression of transforming growth factor (TGF)-α, TGF-β 2 and interleukin 8 messenger RNA in postsurgical and cultured lens epithelial cells obtained from patients with senile cataracts

Nishi¹,

Nishi²,

Wada³

et al. 1999

Graefe's Archive for Clinical and Experimental Ophthalmology

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Immunolocalization of transforming growth factor-a and its receptor in the normal and hyperoxia-exposed neonatal rat retina

Cited by 7 publications

References 19 publications

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina

Survival of Purified Rat PhotoreceptorsIn VitroIs Stimulated Directly by Fibroblast Growth Factor-2

Expression of transforming growth factor (TGF)-α, TGF-β 2 and interleukin 8 messenger RNA in postsurgical and cultured lens epithelial cells obtained from patients with senile cataracts

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