Immunologic significance of increased soluble CD8/CD4 molecules in patients with active systemic lupus erythematosus

Sawada, Shigemasa; Hashimoto, Hiroshi; Iijima, Sumio; Tokano, Yoshiaki; Takei, Masami; Shida, Masako; Obara, Tadashi

doi:10.1002/jcla.1860070303

Cited by 15 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have found increased levels of serum sCD4 in RA and SLE patients, indicating a relevant link to disease activity [13], [39], [40]. In our analysis of clinical serum samples, significantly higher levels of serum sCD4 were found in RA and SLE patients, but not in those with gout.…”

Section: Discussionsupporting

confidence: 48%

Increased Soluble CD4 in Serum of Rheumatoid Arthritis Patients Is Generated by Matrix Metalloproteinase (MMP)-Like Proteinases

et al. 2013

View full text Add to dashboard Cite

Higher soluble CD4 (sCD4) levels in serum have been detected in patients of infectious and chronic inflammatory diseases. However, how and why sCD4 is produced remains poorly understood. We establish sensitive ELISA and WB assays for sCD4 detection in conditioned medium of in vitro cell culture system and serum of chronic inflammatory patients. Serum samples from patients with systemic lupus erythematosus (SLE) (n = 79), rheumatoid arthritis (RA) (n = 59), ankylosing spondylitis (AS) (n = 25), gout (n = 31), and normal controls (n = 99) were analyzed using ELISA for sCD4 detection. Results from each assay were analyzed by the Kruskal-Wallis test. Dunn’s multiple comparison post-test was then applied between groups. We confirm that cells expressing exogenous CD4 produce sCD4 in a constitutive and PMA-induced manner. Importantly, sCD4 production in a heterologous expression system is inhibited by GM6001 and TAPI-0, suggesting receptor shedding by matrix metalloproteinase (MMP)-like proteinases. Moreover, similar findings are recapitulated in human primary CD4+ T cells. Finally, we show that serum sCD4 levels are increased in patients of chronic inflammatory diseases including RA and SLE, but not in those with gout. Intriguingly, sCD4 levels in RA patients are correlated positively with the disease activities and higher sCD4 levels seem to associate with poor prognosis. Taken together, we conclude that CD4 is shed from cell surface by a MMP-like sheddase and sCD4 level is closely related with the inflammatory condition in certain chronic diseases. Hence, sCD4 might be considered an important parameter for RA disease progression with potential diagnostic importance.

show abstract

Section: Discussionsupporting

confidence: 48%

Increased Soluble CD4 in Serum of Rheumatoid Arthritis Patients Is Generated by Matrix Metalloproteinase (MMP)-Like Proteinases

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Leishmania infection, 21 human immunodeficiency virus infection, 22 Sjögren’s syndrome 23 and systemic lupus erythematosus. 24 In these inflammatory diseases, the measured serum sCD4 level could be considered as an index of T‐cell activation. Despite the enthusiasm for studying serum sCD4 in various kinds of autoimmune diseases, there have been no available data regarding sCD4 in BP fluids.…”

Section: Discussionmentioning

confidence: 99%

High levels of interleukin‐8, soluble CD4 and soluble CD8 in bullous pemphigoid blister fluid. The relationship between local cytokine production and lesional T‐cell activities

Sun

Wong

et al. 2000

British Journal of Dermatology

View full text Add to dashboard Cite

show abstract

“…Recent studies have identified several cytokines that play important roles in the immunopathogenesis of various immune disorders, such as SLE [19][20][21]. Other compounds, such as soluble interleukin-2 (IL-2) receptor, soluble CD4 and soluble CD8, have also been examined and are considered to play important roles in the aberrant immune function encountered in SLE [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Significance of stem cell factor and soluble KIT in patients with systemic lupus erythematosus

et al. 1998

Self Cite

View full text Add to dashboard Cite

To determine the significance of stem cell factor (SCF) and soluble KIT (sKIT) in the serum of patients with systemic lupus erythematosus (SLE), levels of SCF and sKIT in patients with SLE were estimated, and their correlations with clinical parameters were examined. The sKIT levels in SLE patients (n = 106) were significantly lower than those in healthy controls (n = 40). A significant negative correlation was found between the SCF and sKIT levels of SLE patients. Although the SCF levels correlated with the titre of anti-RNP antibody, no significant relationship was found between SCF levels and blood cell counts, such as white blood cell, red blood cell and platelet counts. sKIT levels were significantly correlated with the platelet count, and were negatively correlated with the white blood cell count, titre of anti-DNA antibody, and SLE activity index (SLEDAI). sKIT levels were also negatively affected by high doses of corticosteroid. These results indicate that serum sKIT levels may be more closely related than SCF to the haematological abnormalities in SLE patients, and may reflect the clinical status of SLE patients and the effectiveness of high-dose corticosteroid treatment.

show abstract

Immunologic significance of increased soluble CD8/CD4 molecules in patients with active systemic lupus erythematosus

Cited by 15 publications

References 35 publications

Increased Soluble CD4 in Serum of Rheumatoid Arthritis Patients Is Generated by Matrix Metalloproteinase (MMP)-Like Proteinases

Increased Soluble CD4 in Serum of Rheumatoid Arthritis Patients Is Generated by Matrix Metalloproteinase (MMP)-Like Proteinases

High levels of interleukin‐8, soluble CD4 and soluble CD8 in bullous pemphigoid blister fluid. The relationship between local cytokine production and lesional T‐cell activities

Significance of stem cell factor and soluble KIT in patients with systemic lupus erythematosus

Contact Info

Product

Resources

About