Immunological and Molecular study of Toxoplasma gondii from aborted women in Diyala / Iraq

Use of immunological methods to the detection of toxoplasmosis and heat shock protein HSP70 in men

“…1 shows the amplification plot of the real-time PCR reaction for product positive samples for the T. gondii parasite diagnosis by B1 gene, where the (yaxis) represents the dye measurement unit while the (xaxis) represents the thermal cycles of the reaction. This result higher than what recorded by Hernández-Cortazar et al (2016) a ratio (3.84%) by quantitative PCR and (6.92%) when using nested PCR at they examined 161 blood samples for aborted women in Mexico, while lower than Darweesh et al (2018) in Baqubah city/where they recorded (15%) infection rate by used real-time PCR, and with study Badr et al (2016) in Egypt when they used traditional Polymerase chain reaction to identify for T. gondii in aborted women and they recorded (12.5%), as well as with Al-nasrawi et al (2014) when they detected of the parasite in blood samples from aborts woman reviews for Al-Mothana hospital laboratory and record (16%) by using real-time PCR based TaqMan prob. Molecular diagnosis of toxoplasmosis is the most accurate and sensitive because the ability of this methods to diagnose the modern infection of the parasite before the body begins to form antibodies, while the serological examination depends on the formation of antibodies after several weeks from infection of the host parasite (Al-Mosawi et al 2015; Al- x 2 = 51.39 df = 1 P \ 0.05 *The results of the statistical analysis showed a significant difference between the percentage of IgG and IgM under the probability level P \ 0.05 abodi et al 2015), the difference in the results of the B technique between the current study and the other studies is due to differences in methods of extraction of DNA and its methods of work and to the amount of parasite in the sample, in addition to this type of sample under study and population density and surrounding environmental conditions (Al-abodi et al 2015).…”

“…The results showed that positive samples with T. gondii were 39/200 (19.5%) in males students under study, 20(10%) for antibodies IgG and 12(6%) for antibodies IgM, this results refers to the ancient infection more than modern infection, because when results show to IgM in samples, that's refers to (modern infection) present the parasite in the time of examination, while IgG show to ancient infection (Darweesh et al 2018), as explain in a Table 2.…”

“…A-Collection of blood samples (200) blood samples were collected for a random sample of males university students who volunteered to be examined at University of Al-Qadisiyah at 5 ml per sample, blood samples were collected from male students at university after wearing protective medical bra that prevents leakage of liquids, sterile gloves and gags to avoid infection of any potential illness by donors, the samples was divided as follows: (3 ml) was isolated for extracted the serum by using a centrifuge at 3000 cycles/10 min then stored in Eppendorf-tube at temperature -20°C until the time of ELISA testing and investigation of Hps40 (Firouz et al 2014), (2 ml) of venous blood put in a test tube containing an EDTA and store at -80°C until DNA extraction for molecular diagnosis (Darweesh et al 2018;Al-abodi 2018a, b).…”

Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among female population in Duhok province, Iraq

“…These outcomes agreed slightly with results recorded the higher rates of seropositive toxoplasmosis among housewives followed by employee then the student of about 50%, 40.9% and 6.8% respectively. 31 In the current study the outcomes of seropositive rate for age group which started from 16-25 was 30.55, then increased with the increases of the age group till the age 46-55 years at which then decreased to the lower rate of 25%, 0% for both IgG, IgM Abs, respectively. While the higher rate showed among the age group 36-45 years which was 39.74% and 1.28% for both IgG, IgM respectively.…”

Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran

“…However, our study showed higher seroprevalence of IgG and IgM antibodies than most of the above-mentioned research. In two studies conducted in Turkey and Iraq, the neighboring countries of Iran, the seroprevalence of IgG anti-Toxoplasma antibodies in women with spontaneous abortions was 30.6% and 6.6%, while that of IgM was 37.9% and 5.2%, respectively [28,29]. In a global study, the seroprevalence of anti-T. gondii IgG and IgM antibodies in women with a history of abortion and with an abortion in the present pregnancy was reported as 43% and 3% and 33% and 1%, respectively [3].…”