Immunological behavior of enhanced green fluorescent protein (EGFP) as a minor histocomaptibility antigen with a special reference to skin isograft and specific regulation of local graft-versus-host reaction (GvHR)

Riboflavin‐ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells

“…Independent of UV light/pathogen reduction treatment, all animals previously exposed to uGFP RBCs had preferential clearance of uGFP RBCs over control RBCs upon reexposure. Of note, experiments in other model systems have described immune responses to the minor histocompatibility antigen GFP …”

“…Of note, experiments in other model systems have described immune responses to the minor histocompatibility antigen GFP. 33,34 Study limitations must always be considered. These studies were completed in mice, given the advantages afforded by reductionist models.…”

Development and characterization of a new inbred transgenic rat strain expressing DsRed monomeric fluorescent protein

“…Although these models enable transplanted cells to be identified, most are immunogenic and lead to rejection in a few days (Goulding et al 2008). Consequently, the ability to undertake long-term analysis of labeled cells derived from Tg animals from the same strain that express high levels of the transgene while maintaining low levels of immunogenicity is limited (Moloney et al 2010;Pan et al 2009).…”

A transgenic-cloned pig model expressing non-fluorescent modified Plum

“…For example, when transgenic (Tg) rat skin [18] or limbus [19] expressing GFP was transplanted into syngenic inbred rats, the antigenicity of the fluorescent proteins was thought to be the cause of the reported tissue rejection. Similar rejection was reported when GFP-expressing lymphocytes [20] and myoblasts [21] were transplanted into syngenic inbred mice.…”