Immunological relationships betweenSalmonella flagellins and between these and flagellins from other species of Enterobacteriaceae

Abstract

Ten antisera raised in rabbits, against polymeric flagellins from ten different Salmonella serotypes were used to determine the relative cross-activities between salmonella flagellins in monomeric form. The results showed a high degree of cross-reactivity between the antisera (IgG antibodies) and all monomeric flagellins investigated. Consequently, it was possible to detect the ten Salmonella serotypes, after heat depolymerization of flagella using radioimmunometric assay with only one antiserum raised against polymeric flagellin from one serotype. The results also showed that native flagella differed antigenically from repolymerized and monomeric flagellins. This is possibly due to changes in the tertiary structure of sub-units of flagella when depolymerized with acid or heat resulting in unfolding and unmasking of common antigenic determinants. The unfolded and unmasked antigenic determinants were not only common to the Salmonella serotypes investigated, but also to other members of Enterobacteriaceae.

Location of epitopes on Campylobacter jejuni flagella

“…168,1986 on May 12, 2018 by guest http://jb.asm.org/ its structure allow it to polymerize to form a filament of the correct size and helical form, the filament must also be capable of assuming left-and right-handed helices of different dimensions depending on the direction of filament rotation (16,18). This unique structure-function relationship also likely explains the high degree of antigenic crossreactivity between epitopes of denatured monomeric Enterobacteriaceae flagellins reported by Ibrahim et al (15). Interestingly, however, the cross-reactive Enterobacteriaceae flagellin epitopes do not appear to be antigenically related to the cross-reactive Campylobacter flagellin epitopes as antiserum SML2 does not react with Enterobacteriaceae flagellins (A. Lee, S. M. Logan, and T. J.…”

“…Trust, unpublished data). Conformational epitopes also play a major role in the serospecificity of Salmonella flagella (14,15).…”

Detecção rápida de Salmonella Enteritidis em alimentos por ensaio imunoenzimático ELISA

“…Diversas técnicas imunológicas já foram desenvolvidas, usando tanto anticorpos monoclonais como policlonais os quais detectam a maioria dos sorotipos de Salmonella associados à infecção humana [12,18,21,25,33,40]. Estes ensaios estão disponíveis comercialmente na forma de kits [3], geralmente de custo inacessível para a maioria dos laboratórios brasileiros, principalmente para aqueles públicos e oficiais de diagnóstico de toxinfecção alimentar.…”

“…O método convencional de detecção de Salmonella em alimentos envolve etapas de cultura dispendiosas e trabalhosas, consome longo tempo, necessitando-se normalmente de 4 a 5 dias para a confirmação dos resultados [4,6]. Portanto, o emprego de métodos rápidos, simples e confiáveis, é importante tanto no diagnóstico de toxinfecção, como também, e principalmente, no controle de qualidade de alimentos.Diversas técnicas imunológicas já foram desenvolvidas, usando tanto anticorpos monoclonais como policlonais os quais detectam a maioria dos sorotipos de Salmonella associados à infecção humana [12,18,21,25,33,40]. Estes ensaios estão disponíveis comercialmente na forma de kits [3], geralmente de custo inacessível para a maioria dos laboratórios brasileiros, principalmente para aqueles públicos e oficiais de diagnóstico de toxinfecção alimentar.…”

Serology and Genetics of the Flagellar Antigen ofEscherichia coliO157:H7a,7c

“…Disrupted flagella and monomeric flagellin subunits studied with monoclonal antibodies and by enzyme-linked immunosorbent assay techniques exhibit less serological specificity than whole flagella (9,10,35). Monoclonal antibodies specific for H7 have been found to react with the H7 flagellin of E. coli O157 at different intensities than they react with the H7 flagellin of E. coli strain U5/41 (O1:K1:H7), the standard H7 test strain (9); however, no other evidence of their probable nonidentity was shown.…”