Immunological Studies on Antisera of Mice Immunized with Dried or DEPC-treated Ehrlich Ascites Tumor Cells

Fractionation and Properties of Antibodies against Saroma 180

“…Antisera of mice immunized by challenge inoculations of sarcoma 180 cells was prepared as reported previously. 8) In the present experiment, the antisera were obtained from mice (dd mice) which were inoculated with Sarcoma 180 cells (2 x 10 6 cells/mouse/once) by 5 times challenge (antiserum-A) and from those submitted to further 3 times challenge inoculations (1 x 10 6 cells/ mouse/once) (antiserum-B).…”

“…Assay of immune adherence activity, cytotoxicity in vivo and immunodiffusion test of antibodies was performed as reported previously. [6][7][8][9][10] Protein was determined by measuring absorbance at 280 nm or by the Lowry's method.ll) A Hitachi 124 spectrophotometer was used for spectral analysis.…”

“…8 ,9) It was also demonstrated that a considerable mgh titer of antibody against the above cells was found in the antisera of the immunized mice. 8 ) This paper deals with the fractionation and characterization of the antibodies in the antisera of the mice immunized with Sarcoma 180 cells.…”