Immunological Studies on Dermatophytes II. Serological Reactivities of Mannans Prepared from Galactomannans I and II of Microsporum quinckeanum, Trichophyton granulosum, Trichophyton interdigitale, Trichophyton rubrum , and Trichophyton schoenleinii

Abstract

The contribution of terminal galactofuranose residues to the antigenic specificity and to cross-reactivity of galactomannans isolated from five species of dermatophytes, Microsporum quinckeanum, Trichophyton granulosum, T. interdigitale, T. rubrum, and T. schoenleinii, was investigated. Galactofuranose units were removed from galactomannans I and galactomannans II by mild acid hydrolysis. The resulting mannans were tested for serological reactivity with rabbit antiserum to M. quinckeanum by qualitative precipitation in gel and by quantitative complementfixation analyses. Our results showed that, with this antiserum, the galactofuranose residues contributed greatly to the antigenic specificity and to cross-reactivity of the galactomannans II, but these residues were less significant as antigenic determinants in the galactomannans I. We have shown that mannans isolated from three Candida species reacted with rabbit antiserum to M. quinckeanum.

Immunological Studies on Dermatophytes III. Further Analyses of the Reactivities of Neutral Polysaccharides with Rabbit Antisera to Microsporum quinckeanum, Trichophyton schoenleinii, Trichophyton rubrum, Trichophyton interdigitale , and Trichophyton granulosum

“…It has already been shown (8) that the galactofuranose residues contribute greatly to the serological reactivities of this group of polysaccharides. Each of the mannans II studied showed decreased but different reactivities with antiserum to M. quinckeanum, indicating that the mannan chains differ in this group of polysaccharides (8). In the present investigation, it was shown that the antisera varied in their specificity for galactofuranose groups of the galactomannans II.…”

“…The isolation of the polysaccharides and their gross chemical structures have already been reported (1-6). Mannans were prepared from the galactomannan polysaccharides by mild acid hydrolysis (8).…”

“…Removal of galactofuranose residues from these polysaccharides decreased significantly their reactivities with this antiserum. However, reactivities of the mannans II were still measurable by complement fixation (8).…”

Evaluation of Enzyme‐Linked lmmunosorbent Assay for Detection of Molds in Foods

“…To further understand the specificity of the fungal antigenantibody reactions, it was important to determine the antigenic from Sepharose 6B column chromatography were possibly high molecular weight polysaccharides. The major fungal antigenic components isolated in many studies were polysaccharides (Grappel et al, 1968;Hayashi et al, 1978;Schumacher et al, 1975). Most of the fungal filtrates used as antigens for diagnosing mycoses were polysaccharides (Pepys and Longbottom, 1978).…”

Partial purification and characterization of mold antigens commonly found in foods

“…Galactose, mannose, and glucose are the dominant sugars in these fungal polysaccharides (4, 10, 48); therefore, cross-reactions of the antigenantibody among mold species might be due to the presence of these common sugars. Grappel et al (12)(13)(14) isolated galactomannans from Trichophyton species that could react with the antibody against Microsporum quinckeanum. Schumacher et al (44) injected rabbits with polysaccharides isolated from Alternaria tenuis, and the subsequent antibodies reacted with A. fumigatus, Stemphylium spp., and Curvularia spp.…”