Immunologische und enzymatische Aktivität nach Fixierung mit Diimidoester Verbindungen

Halbhuber, Von Karl-Jürgen; Fröber, Rosemarie; Feuerstein, H; Geyer, Günther

doi:10.1016/s0065-1281(80)80084-5

Cited by 7 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypo-osmotic ghosts, however, often reseal at temperatures above 0°C after return to iso-osmoticity as well as under conditions of decreased ionic strength (Lee et al, 1985;Nash & Meiselman, 1985). Membrane isolation A 14 ml portion ofan ice-cold filter solution containing 154 mM-NaCl, 2 mM-EDTA, 1 mM-EGTA and 0.005% of the haemagglutinating polycationic dye Alcian Blue, pH 7.6 (adjusted at room temperature) (Halbhuber et al, 1977) was added to the frozen incubation mixture. The mixture was thawed carefully, with stirring, so that the temperature did not rise above 0-1°C, and then centrifuged for 5 min at 14000 g and 0 'C.…”

Section: Radiolabelling Time Coursementioning

confidence: 99%

Turnover of phosphomonoester groups and compartmentation of polyphosphoinositides in human erythrocytes

Müller¹,

Hegewald²,

Jaroszewicz³

et al. 1986

Biochemical Journal

View full text Add to dashboard Cite

The turnover of phosphomonoester groups of phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] was investigated in human erythrocytes by short-term labelling with [32P]P1. The procedure applied ensured a quantitative extraction of erythrocyte polyphosphoinositides as well as their reliable separation for the determinations of pool sizes and specific radioactivities. The pool sizes of phosphatidylinositol (Ptdlns), PtdIns4P and Ptdlns(4,5)P2 are 25, 11 and 44 nmol/ml of cells respectively. Under steady-state conditions, the phosphorylation fluxes from [y-32P]ATP into PtdIns4P and Ptdlns(4,5)P2 are in the ranges 14-22 and 46-94 nmol -h-1 -ml of cells-' respectively. Only 25-60% of total PtdIns4P and 6-10 O of total PtdIns(4,5)P2 take part in the rapid tracer exchange, i.e. are compartmentalized. In isolated erythrocyte ghosts, the turnover of PtdIns4P approximately corresponds to that in intact erythrocytes, although any compartmentation can be excluded in this preparation. Under the conditions of incubation employed, the tumover of Ptdlns(4,5)P2 is more than one order of magnitude smaller in isolated ghosts than that obtained for intact erythrocytes.

show abstract

Section: Radiolabelling Time Coursementioning

confidence: 99%