Immunology and organic geochemistry

Muyzer, Gerard; Westbroek, Peter; Vrind, J. P. M. de; Tanke, J.; Vrijheid, T.; Jong, Elisabeth W. de Vrind‐de; Bruning, J. W.; Wehmiller, John F.

doi:10.1016/0146-6380(84)90107-4

Cited by 44 publications

(11 citation statements)

References 11 publications

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“…Amino acid analyses were among the first molecular analytical methods to be applied to Mesozoic fossils, including dinosaurs (e.g., refs. []).…”

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

“…Chirality studies were later incorporated into amino acid analyses to test the endogeneity of fossil‐derived amino acids, using the rationale that the ratio of D/L amino acids in proteins from a fossil sample could be used to validate the geological age, and thus ancient source, of these compounds . Additionally, because racemization was correlated to rates of depurination, the degree of racemization was also viewed as a proxy for DNA survival .…”

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

“…Immunological methods, primarily extraction‐based methods such as enzyme linked immunosorbent assays (ELISA), radioimmunoassays, or immunoblot (Western blot) have been applied to Mesozoic fossil materials for many years (e.g., refs. []). These methods rely on the well‐documented specificity (e.g., ref.…”

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

“…The usefulness of an immunological approach to the study of fossils, when proper controls are applied, cannot be overstated. Antibodies may recognize different aspects of epitopes in chemical extractions of fossil tissues (ELISA and immunoblot), and can, for example, differentiate between the native and denatured state of a protein. ELISAs use antibodies to recognize epitopes preserved in chemically extracted material, and is the most sensitive antibody assay.…”

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

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Paleoproteomics of Mesozoic Dinosaurs and Other Mesozoic Fossils

et al. 2019

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Mary Higby Schweitzer would like to dedicate this review to Rune Martinson, a brave warrior in the fight against cancer who left us far too soon.Molecular studies have contributed greatly to our understanding of evolutionary processes that act upon virtually every aspect of living organisms. However, these studies are limited with regard to extinct organisms, particularly those from the Mesozoic because fossils pose unique challenges to molecular workflows, and because prevailing wisdom suggests no endogenous molecular components can persist into deep time. Here, the power and potential of a molecular approach to Mesozoic fossils is discussed. Molecular methods that have been applied to Mesozoic fossils-including iconic, non-avian dinosaurs-and the challenges inherent in such analyses, are compared and evaluated. Taphonomic processes resulting in the transition of living organisms from the biosphere into the fossil record are reviewed, and the possible effects of taphonomic alteration on downstream analyses that can be problematic for very old material (e.g., molecular modifications, limitations of on comparative databases) are addressed. Molecular studies applied to ancient remains are placed in historical context, and past and current studies are evaluated with respect to producing phylogenetically and/or evolutionarily significant data. Finally, some criteria for assessing the presence of endogenous biomolecules in very ancient fossil remains are suggested as a starting framework for such studies.

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“…Amino acid analyses were among the first molecular analytical methods to be applied to Mesozoic fossils, including dinosaurs (e.g., refs. []).…”

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

Section: Protein Studies On Mesozoic Fossilsmentioning

confidence: 99%

See 2 more Smart Citations