Immunomodulation by Hibiscus rosa-sinensis: Effect on the Humoral and Cellular Immune Response of Mus Musculus

Abstract

In West India, the Hibiscus rosa-sinensis L. (Malvaceae) is traditionally used as tea as a natural diuretic. Extract of this plant contains Vitamin C and is used traditionally as a mild medicine. In spite of a long history of traditional medicinal value of H. rosa-sinensis still no data are available for immunomodulatory activity. In present investigation, aqueous extract of H. rosa-sinensis (AEHrs) (500 mg kg(-1) BW) was intraperitoneally (IP) injected to the male Swiss albino mice (Mus musculus) to evaluate the immunomodulatory property of extract. In addition for evaluation of phytochemical constituents of flowers of H. rosa-sinensis HPTLC was performed. The crude extract of H. rosa-sinensis has immunomodulatory activity. After the 15 days treatment, the number of plaque forming cells increased by 0.6%, antibody titre was increased 38.15% and significant increase of 52% was observed in DTH response. At the same concentration of dose the level of serum IL-1alpha enhanced significantly (14.27%) whereas a considerable decrease (32.70%) in the concentration of IL-2 was observed among AEHrs treated mice in comparison to the control mice. HPTLC chromatogram revealed that H. rosa-sinensis posses alkaloid (Rf 0.93) and flavonoids (Rf 0.02, 0.06, 0.14) on the basis of Rf values. Results of investigation supports for the immunomodulatory activity of H. rosa-sinensis aqueous extract.

Components in aqueous Hibiscus rosa-sinensis flower extract inhibit in vitro melanoma cell growth

“…For example, recent studies found that Hibiscus polyphenols inhibit melanoma cell growth and viability 10 . However, while H. rosa-sinensis flowers are commonly used to make medicinal tea,11, 12 these previous studies were performed with organic solvent extracts of leaves from different strains, namely Hibiscus sabdariffa 10 …”

Anthocyanin, tartaric acid, ascorbic acid of roselle flower (Hibiscus sabdariffa L.) for immunomodulatory adjuvant therapy in oral manifestation coronavirus disease-19: An immunoinformatic approach

“…Anti-inflammatory and antioxidant properties of H. sabdariffa flower play an important role in immunomodulatory activity (Fakeye, 2008;Mishra et al, 2012). The intensity of the inflammatory response is controlled by the recruitment of inflammatory cells into the inflammatory lesion (Zhang and An, 2007).…”

Review: Aktivitas Imunostimulan dari Famili Malvaceae

“…Hibiscus rosa-sinensis L. atau bunga sepatu merupakan tanaman yang memiliki khasiat empiris, sebagai obat keputihan, dan diuretik alami. Hibiscus rosa-sinensis L. mengandung senyawa alkaloid, flavonoid, polifenol, dan asam amino [17], [35], [36].…”

“…Senyawa yang berperan dalam respon imun terhadap hewan uji yaitu flavonoid. Flavonoid mampu meningkatkan titer antibodi secara signifikan serta meningkatkan respon humoral, dengan cara merangsang subset makrofag dan B-limfosit yang berperan dalam sintesis antibodi [17].…”