Immunomodulation in systemic lupus erythematosus: induction of M2 population in monocyte-derived macrophages by pioglitazone

Mohammadi, Saeed; Saghaeian-Jazi, Marie; Sedighi, Sima; Memarian, Ali

doi:10.1177/0961203317701842

Cited by 52 publications

(36 citation statements)

References 38 publications

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“…So, in children with SLE, M1 macrophagelike monocytes played an important role in inflammation in children with SLE and had correlation with the severity of disease. This was consistent to the researches by Mohammadi et al 11 and Li et al 4 Monocytes/macrophages are important in the development of SLE. M1 macrophage-like Severe SLE group (494 ± 269) × 10 6 L −1 40.7% ± 26.0%** # (189 ± 156) × 10 6 L −1 ** # 59.2% ± 26.0%** # (303 ± 232) × 10 6 L −1 SLE: systemic lupus erythematosus.…”

Section: Discussionsupporting

confidence: 93%

The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus

et al. 2019

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Monocytes/macrophages are important in the development of systemic lupus erythematosus. To research M1 and M2 macrophage-like monocytes in the peripheral blood of children with systemic lupus erythematosus and explore the clinical significance, M1 and M2 macrophage-like monocytes, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, and interleukin-18 are tested in the peripheral blood of children with systemic lupus erythematosus by flow cytometry. A correlation analysis is made between M1 and M2 macrophage-like monocytes and erythrocyte sedimentation rate and C-reactive protein. As we found, the absolute number and percentage of M1 macrophage-like monocytes (CD163-CD14 +) in macrophage-like monocytes (CD14 +) in peripheral blood of the severe systemic lupus erythematosus group were higher than those of the control group and the mild-moderate systemic lupus erythematosus group (F = 28.4, 21.7, 122, 81.7; P < 0.05). But there was no obvious difference between these three groups in terms of the absolute number of M2 macrophage-like monocytes (CD163 + CD14 +). The absolute number and percentage of M1 macrophage-like monocytes in macrophage-like monocytes had positive correlation with C-reactive protein and erythrocyte sedimentation rate (r = 0.46, 0.44, 0.367, 0.47; P < 0.05); whereas, the absolute number and percentage of M2 macrophage-like monocytes in macrophage-like monocytes had negative correlation with CRP and erythrocyte sedimentation rate (r =-0.47,-0.45,-0.47,-0.32; P < 0.05). Thus, M1 macrophage-like monocytes have effective impact on inflammation in children with systemic lupus erythematosus. M2 macrophage-like monocytes, to a large extent, have the opposite functions compared to M1 macrophage-like monocytes. In children with systemic lupus erythematosus, macrophages play important role in the development of systemic lupus erythematosus and M1 macrophage-like monocytes have functions in active systemic lupus erythematosus and they can induce the inflammation and have correlation with severity of systemic lupus erythematosus.

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Section: Discussionsupporting

confidence: 93%

The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus

et al. 2019

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“…Production of the Th2 cytokine, IL-4, can polarize macrophage differentiation into M2 type macrophages [ 25 , 26 , 27 ]. These polarized M2 macrophages can directly produce and secrete TGF-β1, which can exacerbate the fibrotic process after radiation exposure [ 26 , 28 ]. TGF-β1 produced by the macrophages has also been linked to the production of myofibroblasts via the process of macrophage-myofibroblast transition in a mouse model of unilateral ureteric obstruction [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues

et al. 2017

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Pelvic radiation for cancer therapy can damage a variety of normal tissues. In this study, we demonstrate that radiation causes acute changes to pelvic fibroblasts such as the transformation to myofibroblasts and the induction of senescence, which persist months after radiation. The addition of the manganese porphyrin, MnTE-2-PyP, resulted in protection of these acute changes in fibroblasts and this protection persisted months following radiation exposure. Specifically, at two months post-radiation, MnTE-2-PyP inhibited the number of α-smooth muscle actin positive fibroblasts induced by radiation and at six months post-radiation, MnTE-2-PyP significantly reduced collagen deposition (fibrosis) in the skin and bladder tissues of irradiated mice. Radiation also resulted in changes to T cells. At two months post-radiation, there was a reduction of Th1-producing splenocytes, which resulted in reduced Th1:Th2 ratios. MnTE-2-PyP maintained Th1:Th2 ratios similar to unirradiated mice. At six months post-radiation, increased T cells were observed in the adipose tissues. MnTE-2-PyP treatment inhibited this increase. Thus, MnTE-2-PyP treatment maintains normal fibroblast function and T cell immunity months after radiation exposure. We believe that one of the reasons MnTE-2-PyP is a potent radioprotector is due to its protection of multiple cell types from radiation damage.

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“…Additional markers for M1 macrophages like iNOS, IL-6, SOSC3, and TNF-α and ARG1 and CCL24 for M2 macrophages have been reported (Qin et al, 2017). Furthermore, the expression pattern of interleukin (IL)-10, IL-12, transforming growth factor β1, and TNF-α is important for investigating M1/M2 polarization (Mohammadi et al, 2017a). These mesenchymal cells play an important role in cell support and homeostasis.…”

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confidence: 99%

Melittin Induced G1 Cell Cycle Arrest and Apoptosis in Chago-K1 Human Bronchogenic Carcinoma Cells and Inhibited the Differentiation of THP-1 Cells into Tumour- Associated Macrophages

Tipgomut

Wongprommoon

Takeo

et al. 2018

Asian Pac J Cancer Prev

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Background: Bronchogenic carcinoma (lung cancer) is one of the leading causes of death. Although many compounds isolated from natural products have been used to treat it, drug resistance is a serious problem, and alternative anti-cancer drugs are required. Here, melittin from Apis mellifera venom was used, and its effects on bronchogenic carcinoma cell proliferation and tumour-associated macrophage differentiation were evaluated. Methods: The half maximal inhibitory concentration (IC50) of melittin was measured by MTT. Cell death was observed by annexin V and propidium iodide (PI) co-staining followed by flow cytometry. Cell cycle arrest was revealed by PI staining and flow cytometry. To investigate the tumour microenvironment, differentiation of circulating monocytes (THP-1) into tumour-associated macrophages (TAMs) was assayed by sandwich-ELISA and interleukin (IL)-10 levels were determined. Cell proliferation and migration was observed by flat plate colony formation. Secretion of vascular endothelial growth factor (VEGF) was detected by ELISA. The change in expression levels of CatS, Bcl-2, and MADD was measured by quantitative RT-PCR. Results: Melittin was significantly more cytotoxic (p < 0.01) to human bronchogenic carcinoma cells (ChaGo-K1) than to the control human lung fibroblasts (Wi-38) cells. At 2.5 µM, melittin caused ChaGo-K1 cells to undergo apoptosis and cell cycle arrest at the G1 phase. The IL-10 levels showed that melittin significantly inhibited the differentiation of THP-1 cells into TAMs (p < 0.05) and reduced the number of colonies formed in the treated ChaGo-K1 cells compared to the untreated cells. However, melittin did not affect angiogenesis in ChaGo-K1 cells. Unlike MADD, Bcl-2 was up-regulated significantly (p < 0.05) in melittin-treated ChaGo-K1 cells. Conclusion: Melittin can be used as an alternative agent for lung cancer treatment because of its cytotoxicity against ChaGo-K1 cells and the inhibition of differentiation of THP-1 cells into TAMs.

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Immunomodulation in systemic lupus erythematosus: induction of M2 population in monocyte-derived macrophages by pioglitazone

Cited by 52 publications

References 38 publications

The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus

The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus

The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues

Melittin Induced G1 Cell Cycle Arrest and Apoptosis in Chago-K1 Human Bronchogenic Carcinoma Cells and Inhibited the Differentiation of THP-1 Cells into Tumour- Associated Macrophages

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