Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1

Fischer, Geferson; Conceição, Fabrício Rochedo; Leite, Fábio Pereira Leivas; Dummer, Luana Alves; Vargas, Gilberto D’Ávila; Hübner, Sílvia de Oliveira; Dellagostin, Odir A.; Paulino, Niraldo; Paulino, Amarilis Scremin; Vidor, Telmo

doi:10.1016/j.vaccine.2006.10.005

Cited by 91 publications

(72 citation statements)

References 33 publications

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“…These substances are found in green propolis (marcucci et al, 2001;cuSHnie;lamB, 2005). It is worth noting that in a study performed by our group (fiScHer et al, 2007) aiming at characterizing the immunomodulator effect of the same green propolis ethanolic extract evaluated in this study regarding its antimicrobial activity, a chromatographic analysis (High Performance Liquid Chromatography -HPLC) showed high levels of phenolic compounds and cinnamic acid and its derived. In this extract, the flavonoids corresponded to 22.37% of the dried extract (fiScHer et al, 2007).…”

Section: Discussionmentioning

confidence: 54%

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

et al. 2012

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During the cooling process of embryonated eggs, there is a natural air flux from the surface to the inner part of the eggs, carrying contaminants such as bacteria and fungi through the shell's pores, infecting embryos and resulting in the inability to hatch or poor chick quality. Formaldehyde, a toxic product, is still the most used disinfectant for embryonated eggs in the aviculture industry. In order to evaluate the antimicrobial activity of the green propolis ethanolic extract as an alternative to formaldehyde, 140 hatching eggs from laying hens were collected and submitted to disinfection with five different treatments: T1 -without disinfection; T2 -formaldehyde fumigated eggs; T3, T4 and T5 disinfection by immersion in propolis solution in the concentrations of 2,400 µg, 240 µg and 24 µg, respectively. The contamination levels by total mesophiles and fungi of the egg shells (Aspergillus sp. and other moulds) after disinfection with propolis were lower than when compared to the control without disinfection. In comparison with formaldehyde, the 240 µg and 24 µg propolis concentrations did not differ regarding antibacterial activity, but for antifungal activity the 2,400 µg and 240 µg concentrations were more efficient. The 2,400 µg and 240 µg propolis treatments presented a hatching rate of 94.1%, compared to only 84.6% for the formaldehyde treatment. The green propolis ethanolic extract presented antibacterial and antifungal activities in embryonated eggs showing that it can be a new natural disinfectant product substituting formaldehyde.KEY WORDS: Propolis, embryonated eggs, formaldehyde, disinfectants. RESUMO PRÓPOLIS: UM PRODUTO NATURAL COMO ALTERNATIVA PARA DESINFECÇÃO DE OVOS EMBRIONADOS PARA INCUBAÇÃO. Durante o processo de resfriamento dos ovos embrionados, há um fluxo natural de ar da superfície para o interior dos ovos carreando contaminantes como bactérias e fungos, por meio dos poros da casca, infectando o embrião e resultando na inabilidade para eclodir e pintinhos de má qualidade. O formaldeído que é um produto tóxico ainda é o desinfetante mais utilizado para a desinfecção de ovos embrionados pela indústria avícola. Para avaliar a atividade antimicrobiana do extrato etanólico da própolis verde, como alternativa ao formaldeído, foram coletados 140 ovos de ninhos de matrizes de frango de corte submetidos à desinfecção com cinco tratamentos: T1 -sem desinfecção; T2 -ovos fumigados com formaldeído; T3, T4 e T5 desinfetados por imersão com solução de própolis nas concentrações de 2.400 µg, 240 µg e 24 µg, respectivamente. Os níveis de contaminação da casca dos ovos por mesófilos totais e fungos (Aspergillus e outros bolores), após a desinfecção com própolis, foram menores quando comparados ao controle. Na comparação ao tratamento com formaldeído as concentrações de própolis com 240 µg e 24 µg não diferiram para atividade antibacteriana, já para atividade antifúngica, 2,4 mg e 240 µg foram superiores. Com relação à eclodibilidade dos ovos, após 21 dias de incubação, os tratamentos de própolis...

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Section: Discussionmentioning

confidence: 54%

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

et al. 2012

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“…This may be accompanied by increased humoral and cellular immune response. Other researchers have shown that propolis can increase the phagocytic capacity of macrophages 27 .…”

Section: Angiogenesismentioning

confidence: 97%

Effect of red propolis on hamster cheek pouch angiogenesis in a new sponge implant model

Melo

Alves²,

Silva³

et al. 2018

Acta Cir. Bras.

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Effect of red propolis on hamster cheek pouch angiogenesis in a new sponge implant model 1 Abstract Purpose: To evaluate the effects of red propolis on cheek pouch angiogenesis in a hamster new model sponge implant. Methods: Forty eight animals divided into eight groups. (Groups I-IV), the animals were treated for 15 days before and 10 days after sponge implantation. (Groups V-VIII), the animals were treated for 10 days after sponge implantation (GI and GV: red propolis 100 mg/kg, GII and GVI: celecoxib 20 mg/kg, GIII and GVII: 1% gum arabic 5 mL/kg, GIV and GVIII: distilled water 5 mL/kg). On the 11th day of implantation, the animals were anesthetized for stereoscopic microscopic imaging and morphometric quantification of angiogenesis (SQAN), followed by histopathological evaluation (H&E). Results: In the SQAN analysis, no significant difference was found between the groups. However, on histology, propolis was found reduce the population of mastocytes in the qualitative analyses (p = 0,013) in the quantitative analyses to reduce the number of blood vessels (p = 0,007), and increase the macrophage count (p = 0,001). Conclusion: Red propolis inhibited inflammatory angiogenesis when administered before andcontinuously after sponge implant, and was shown to have immunomodulating effects on inflammatory cells (mastocytes and macrophages) in a new sponge implant hamster model.

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“…Therapeutic administration activates lymphocytic function. Proplis increases the cellular immune-response through the increase of mRNA for interferon-c and activates these cells to produce cytokines (Fischer et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of bee venom or proplis on molecular and parasitological aspects of Schistosoma mansoni infected mice

et al. 2014

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The present study was performed to elucidate the efficacy of Apis mellifera L bee venom (BV) or proplis (200 mg/kg orally for three consecutive days) on Schistosoma mansoni infected mice. The results recorded reduction in the total worm burden, numbers of immature eggs and the ova count in hepatic tissue in BV (sting or injection) or proplis treated groups as compared to the infected group. Histological examination illustrated a significant increase (P B 0.05) in the diameter of hepatic granuloma in BV treated groups (272.78 and 266.9, respectively) and a significant decrease in proplis treated mice (229.35) compared with the infected group (260.67). Electrophoretic pattern of RNA showed a decrease in mean of maximal optical density in liver and intestine of S. mansoni infected mice treated with bee venom (sting or injection) as compared with infected group. Flow cytometry analyses of RNA or apoptotic percentage of worms recovered from BV sting (19 and 49 % respectively); BV injected (20.5 and 51.17 %, respectively) and proplis (35 and 23.93 %, respectively) groups were compared with S. mansoni infected group (37.87 and 39.21 %, respectively). It can be concluded that administration of bee venom or proplis are effective in case of S. mansoni infection. Although bee venom cause increase of granuloma diameter and this might be due to venom concentration and further studies are required to avoid such harmful effect.

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Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1

Cited by 91 publications

References 33 publications

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

Propolis: a natural product as an alternative for disinfection of embryonated eggs for incubation

Effect of red propolis on hamster cheek pouch angiogenesis in a new sponge implant model

Effect of bee venom or proplis on molecular and parasitological aspects of Schistosoma mansoni infected mice

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