Immunomodulatory activity of biopolymeric fraction BOS 2000 from <i>Boswellia serrata</i>

Khajuria, Anamika; Gupta, Amit; Suden, Pankaj; Singh, Surjeet; Malik, Fayaz; Singh, Jaswant; Gupta, B. K.; Suri, K. A.; Srinivas, V. K.; Ella, Krishna M.; Qazi, Ghulam N.

doi:10.1002/ptr.2320

Cited by 36 publications

(20 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 72 h, 20 µL of MTT solution (5 mg/mL) were added to each well and incubated for 4 h. The plates were centrifuged (1400 × g, 5 min) and the untransformed MTT was removed carefully by pipetting. To each well, 100 µL of a DMSO working solution (192 µL DMSO with 8 µL 1 M HCl) was added, and the absorbance was evaluated in an ELISA reader at 570 nm after 15 min (Khajuria et al, 2008[16]).…”

Section: Methodsmentioning

confidence: 99%

Immunomodulatory Activity of Bioactive Fraction (PBC) from Phyllostachys bambusoides

Kumar¹,

Sharma²,

Kumar³

2017

View full text Add to dashboard Cite

Background:The lack of vaccines and limitations of currently available strategies demand a need to develop safe and efficacious immunomodulators. Phyllostachys bambusoides is traditionally used for various autoimmune and infectious disorders, a property possibly attributable to presence of flavonoids like orientin and iso-orientin. Objective: the objective of this study was, to search a potent immunomodulator which elicit both Th1 and Th2 immune response. Methods: The animals were (Balb/c) treated with the bioactive fraction (PBC) from P. bambusoides (100 and 200 mg/kg body weight) for 14 days with SRBC (Sheep Red Blood Cells) as an antigen. Haemagglutination antibody (HA) titre, delayed type hypersensitivity (DTH) reaction, phagocytic index, NO production, analysis of cytokines in serum and CD80/ CD86 population in spleen. Results: PBC significantly enhanced the expression of IgM and IgG titre and DTH response in a dose dependant manner after 24 and 48 h in BALB/c mice with a maximum response at 200 mg/Kg. Besides humoral and cell mediated immunity, it also enhanced phagocytic index, nitric oxide production, which further leads to protection against Candida albicans infection. It also, enhanced the expression of CD80 and CD86 in splenic cells. Conclusion: Taken together these in vitro and in vivo data, our results suggest that PBC acts as an effective immunostimulator which specially enhances macrophage function during infection. This further supports the role of PBC in immunopharmacologic applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Immunomodulatory Activity of Bioactive Fraction (PBC) from Phyllostachys bambusoides

Kumar¹,

Sharma²,

Kumar³

2017

View full text Add to dashboard Cite

show abstract

“…Other adjuvants such as monophosphoryl lipid A, ISCOMS, QS-21 a purified saponin from bark of Quillaja saponaria Molina, and Syntex Adjuvant Formulation (SAF) are being investigated for development of better and safer adjuvants (14). Plant based immunomodulators are being considered as one option (15)(16)(17) (18)(19)(20)(21). Attempts to organize adjuvants in grouped categories in order to facilitate adjuvant selection have sometimes been difficult because of multiple and overlapping biological effects of many adjuvants.…”

Section: Types and Classification Of Adjuvantsmentioning

confidence: 99%

Vaccine Adjuvants: The Current Necessity of Life

Gupta

Chaphalkar

2015

Shiraz E-Med J

Self Cite

View full text Add to dashboard Cite

Context:Vaccination is considered as the most cost effective method for the prevention of human diseases. For this prevention method, we need certain substances to increase or boost the antibody as well as cell-mediated immune response against various bacterial as well as viral pathogens. Until now, alum was considered as the safest adjuvant for human use, licensed by the United States Food and Drug Administration. Due to the poor adjuvanticity of alum, conventional vaccines require multiple recall injections, at different time intervals, to attain or sustain the optimal immune response. The present review discusses about the necessity of adjuvants for vaccines. Evidence Acquisition: A number of factors such as slow release of antigen (depot effect), more efficient delivery of antigen to draining lymph nodes, non-specific activation of antigen-presenting cells or of B and/or T-lymphocytes, increased uptake of antigen by antigenpresenting cells or increased recruitment of immune cells to the site where the antigen is present, can contribute to increased immune responses to immunization. Many of these factors involve the interaction of various immune system components and specific anatomical features, making them difficult to replicate in model systems in vitro. Results: Despite the development of many potent adjuvant formulations with vaccine antigen during the last 80 -90 years, aluminum compounds are still the only approved adjuvants used for routine human vaccines. Conclusions: Based on pre-clinical and preliminary clinical observations, it appears that the range of adjuvants accepted for human vaccines will expand in the coming years.

show abstract

“…Extract of Boswellia Serrata or β-boswellic acid has been reported to inhibit hypersensitivity reactions by regulating both the humoral and cellular immune systems They decrease primary antibody synthesis, inhibit polymorphonuclear leukocyte proliferation and infiltration, enhance the phagocytotic function of macrophages, and suppress the classical and alternate complement pathways [254,255] and suppress the inflammation process, one of the critical pathological features in asthma. It has been shown that β-boswellic acid inhibits the production of proinflammatory cytokines, including TNF, IL-1, IL-2, IL-6, IL-12 and IFN-γ by suppressing the activation of NF-κB [256].…”

Section: Boswellia Serratamentioning

confidence: 99%