Immunomodulatory activity of shikimic acid and quercitin in comparison with oseltamivir (Tamiflu) in an “in vitro” model

Bertelli, A.; Mannari, Claudio; Santi, Silvia De; Filippi, C.; Migliori, Massimiliano; Giovannini, Luca

doi:10.1002/jmv.21072

Cited by 24 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shikimic acid, one of the primary components of oseltamivir, alone and in combination with quercetin were evaluated in comparison with oseltamivir in an in vitro model in terms of their ability to modulate the expression of IL-6 and IL-8 as immunomodulatory cytokines from peripheral blood mononuclear cells (PBMCs) [106]. IL-8 attracts T-cells to the site of infection [107] and applies indirect antiviral activity due to the recruitment of leukocytes [108].…”

Section: Anti-inflammatory and Immunomodulatory Effectsmentioning

confidence: 99%

See 1 more Smart Citation

Quercetin as a Natural Therapeutic Candidate for the Treatment of Influenza Virus

et al. 2020

View full text Add to dashboard Cite

The medical burden caused by respiratory manifestations of influenza virus (IV) outbreak as an infectious respiratory disease is so great that governments in both developed and developing countries have allocated significant national budget toward the development of strategies for prevention, control, and treatment of this infection, which is seemingly common and treatable, but can be deadly. Frequent mutations in its genome structure often result in resistance to standard medications. Thus, new generations of treatments are critical to combat this ever-evolving infection. Plant materials and active compounds have been tested for many years, including, more recently, active compounds like flavonoids. Quercetin is a compound belonging to the flavonols class and has shown therapeutic effects against influenza virus. The focus of this review includes viral pathogenesis as well as the application of quercetin and its derivatives as a complementary therapy in controlling influenza and its related symptoms based on the targets. We also touch on the potential of this class of compounds for treatment of SARS-COV-2, the cause of new pandemic.

show abstract

Section: Anti-inflammatory and Immunomodulatory Effectsmentioning

confidence: 99%

“…It was shown that shikimic acid alone was not able to modulate innate immunity in terms of antiviral activity. However, its combination with quercetin, even at low doses, effectively modulated innate immunity by increasing the IL-8 and IL-6 levels [106].…”

Section: Anti-inflammatory and Immunomodulatory Effectsmentioning

confidence: 99%

Quercetin as a Natural Therapeutic Candidate for the Treatment of Influenza Virus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The observed results may be correlated with the high levels of shikimic acid found in red berries. Shikimic acid was found effective, even in low doses, in the modulation of leukocyte activity (Bertelli et al, 2008 ). This compound, either alone or in combination with quercitin, was able to modulate the release of IL-6 and IL-8 from PBMCs.…”

Section: Resultsmentioning

confidence: 99%

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

et al. 2016

View full text Add to dashboard Cite

Hypericum androsaemum, also known as Tutsan, is a small evergreen shrub common in the Mediterranean basin where it is traditionally used as diuretic and hepatoprotective herbal drug. This plant possesses the peculiarity to produce fleshy and berry-like fruits that ripen from red to shiny black. In the present work, the chemical constituents of methanolic extracts and infusions of red and black fruits were analyzed by HPLC, and correlated with their antioxidant properties which were evaluated by the DPPH, β-Carotene/linoleic acid, and hypochlorous acid tests. In addition, the red pigment of the fruit was isolated by column chromatography and structurally elucidated by NMR. Results showed that H. androsaemum fruits contain high amounts of shikimic and chlorogenic acids, while their color was given by a tetraoxygenated-type xanthone, reported for the first time in Hypericum species. The red berries infusion gave the highest content of total phenolic compounds, DPPH, and hypochlorous acid scavenging activity, and β-carotene bleaching. Cytotoxicity of the berries extracts on three human tumor cell lines (malignant melanoma, breast adenocarcinoma, and colon carcinoma) was evaluated by MTT assay, and relevant inhibition on colon carcinoma cells (IC50 value of 8.4 μg/mL) was found. Finally, the effects of red berries extract on the immune system were evaluated by peripheral blood mononuclear cell (PBMC) proliferation assay that revealed a strong stimulation on lymphocytes at low doses (0.4–6 μg/mL).

show abstract

“…The available literature reveals that shikimic acid and pinitol possess immunomodulatory and antidiabetic activities respectively [11,12] . Thus, shikimic acid and pinitol (isolated from A. pindrow aerial parts) were taken as bioactive markers to standardize the plant material.…”

Section: Abies Pindrowmentioning

confidence: 99%

Quantitative Determination of Shikimic Acid and Pinitol in Abies pindrow Aerial Parts Using TLC

Kumar¹,

Kumar²

2016

ijps

View full text Add to dashboard Cite

Kumar and Kumar: TLC Densitometry of Abies pindrow on the Basis of PhenolsAbies pindrow Royle (Himalayan Fir; Pinaceae) has been traditionally used in the treatment of various ailments and reported to contain cyclic polyols as major class of phytoconstituents. But no work has ever been carried out to standardize A. pindrow on the basis of cyclic polyols. Thus, it was considered worthwhile to determine quantitatively contents of main cyclic polyols, i.e., shikimic acid and pinitol in A. pindrow aerial parts using TLC densitometry. Shikimic acid and pinitol were resolved on TLC plates using solvent systems, chloroform:methanol:formic acid (14:6:1) and chloroform:methanol (3:2), respectively. The shikimic acid and pinitol were derivatized with anisaldehyde-sulphuric acid reagent and ammonical silver nitrate reagent respectively. The spots of shikimic acid and pinitol were visualized by heating derivatized plates. The plates were then scanned in TLC scanner at 500 nm. The contents of shikimic acid and pinitol were estimated to be 0.0511% and 0.0987% w/w, respectively, in A. pindrow aerial parts. The developed TLC densitometric methods for estimation of cyclic polyols in A. pindrow aerial parts were found to be precise, accurate and specific on the basis of validation parameters.Key word: Abies pindrow, Pinitol, Shikimic acid, TLC densitometry.Abies pindrow Royle (Silver Fir; family: Pinaceae) is an Indian traditional plant, which is distributed throughout Western Himalayas [1] . The plant has long history of use as anxiolytic, analgesic, antidiabetic and antiinflammatory [2] . The traditional uses of A. pindrow have been validated by scientific studies, but these studies employed crude uncharacterized extracts [3][4][5] . Sporadic phytochemical work has been carried out on the plant. Chalcone glycosides, flavonoids, fatty acids, hydrocarbons and terpenoids have been isolated from A. pindrow [6][7][8][9] . As the case with most plant drugs, A. pindrow has not been standardized on the basis of marker compounds. Marker-based standardization is important for reproducibility of therapeutic efficacy and safety of the plant. High Performance Thin Layer Chromatography (HPTLC) has gained popularity in standardizing plants by estimating the content of, preferably, bioactive constituents. Thus, the present investigation was undertaken to estimate the contents of two main cyclic polyols constituents of A. pindrow using TLC densitometry. Methanol, chloroform (E-Merck, Delhi, India), and formic acid (Ranbaxy Laboratory Chemicals, Mumbai, India), of AR grade, sulphuric acid, glacial acetic acid (E-Merck Ltd., Mumbai), sodium hydroxide, silver nitrate, ammonia solution and anisaldehyde (S.D. Fine Chemicals, Mumbai) were used in present investigations. Shikimic acid and pinitol, isolated from A. pindrow aerial parts, were used as marker compounds. The isolated compounds have been characterized by 1 HNMR and 13 CNMR spectral analysis.The stock solutions of marker compounds were prepared by separately dissolving accurately weighed 5 m...

show abstract

Immunomodulatory activity of shikimic acid and quercitin in comparison with oseltamivir (Tamiflu) in an “in vitro” model

Cited by 24 publications

References 32 publications

Quercetin as a Natural Therapeutic Candidate for the Treatment of Influenza Virus

Quercetin as a Natural Therapeutic Candidate for the Treatment of Influenza Virus

Polar Constituents and Biological Activity of the Berry-Like Fruits from Hypericum androsaemum L.

Quantitative Determination of Shikimic Acid and Pinitol in Abies pindrow Aerial Parts Using TLC

Contact Info

Product

Resources

About