Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro

Abstract

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. Macrophage cells separated form peritoneal fluid used RPMI medium. Phagocytosis activity and phagocytosis capacity of macrophages were performed in vitro using latex beads that suspended in phosphate buffered saline (PBS). The antioxidant activity was measured by spectrophotometry technique with 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution. All treatments were done three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytosis activity followed by chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetate extract. The ethyl acetate extract was also the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract has a high immunomodulatory activity and antioxidant activity which contained phenolics, flavonoids, tannins, and terpenoids.

Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro

“…In addition, the bacteria were shown in purplish pink on the cytoplasm. (17) The phagocytic activity of macrophages was observed using a light microscope at 400× magnification, whereas the phagocytic capacity was observed under 1000× magnification. The phagocytic activity and phagocytic capacity were calculated using the formula in this reference.…”

Cyclea barbata L. Miers.: Penggunaan tradisional, fitokimia, dan aktivitas farmakologi

“…Pemberian tiga ekstrak daun C. barbata pada kultur sel dapat meningkatkan aktivitas fagositosis dan kapasitas fagositosis makrofag. Ekstrak etil asetat daun C. barbata pada konsentrasi 100 µg/ml merupakan konsentrasi yang optimal dalam meningkatkan aktivitas fagositosis dan kapasitas fagositosis sel makrofag (Mahadi et al, 2018).…”

Formulation of Antioxidant Gel From Standardized Green Cincau (Cyclea Barbata L. Miers) Ethanolic Extract

“…Flavonoids also offer disease resistance; these are induced in response to UV-B radiation and provide organ protection by absorbing in the wavelength range of 280-350 nm. They also function as reactive oxygen species (ROS) scavengers [5].…”

“…Green cincau (Cyclea barbata L. Miers) is a plant that people use as traditional medicine and can be processed into beverages [4]. Green cincau has antioxidant activity that can reduce free radicals [5]. Ethanolic extracts of Green cincau that have been obtained from standardized extract so is the quality of the extract has the antioxidant activity of the extract determined.…”

“…Green cincau has lipoxygenase inhibitory activity [3], macrophage migration on burn wound [6], protective effects aspirin-induced gastric ulcer [7], protect from ROS in smoke, and antioxidant activity [5,8,9]. Green cincau leaves, besides containing flavonoids, also contain alkaloids, saponins, tannins, and steroids/triterpenoids [10].…”