Immunomodulatory and In vivo Antiplasmodial Activities of Propolis Extracts

Syamsudin,; Dewi, Rita Marleta; Kusmardi,

doi:10.3844/ajptsp.2009.75.79

Cited by 12 publications

(2 citation statements)

References 14 publications

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“…These kind of changes will further influence several cell functions including the immune response (Ramiro-Puig and Castle, 2009). Propolis is an example of extract rich in antioxidant phenolics compounds, which is also a potential immunomodulatory agent (Syamsudin et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Preliminary Study on Immunomodulatory Effect of Sarang-Semut Tubers Myrmecodia tuberosa and Myrmecodia pendens

Hertiani¹,

Sasmito²,

Sumardi³

et al. 2010

OnLine Journal of Biological Sciences

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Problem Statement: Sarang-Semut tubers, Myrmecodia tuberosa and M. pendens (Rubiaceae) are both medicinal plants originating from Papua which have broad range of therapeutic values including those to improve vitality. Nevertheless, no scientific proof is available so far on their immunomodulatory effect. The purpose of this study was to reveal the potency of Sarang-Semut tubers as immunomodulatory agent by evaluating their effects on Balb/c mice lymphocytes proliferation and macrophage phagocytosis by in vitro techniques. Approach: Bioactivity assays were performed on the raw extracts and fractions. Extracts were obtained by macerating the pulverized samples in ethanol 95%, followed by successive fractionation to yield n-hexane, ethyl acetate and water fractions. Series of extracts and fractions were made to concentration 10, 20, 50 and 100 µg mL −1 by adding 0.5% Tween 80. Lymphocytes proliferation was observed by the MTT reduction method and analyzed using microplate reader at 550 nm. Macrophage phagocytosis activity was determined based on the number of latex beads uptake by the macrophage cells. Results: All extracts and fractions significantly increased the lymphocytes proliferation and macrophage phagocytosis activity in comparison to negative control. The ethyl acetate fraction of M. pendens (50 µg mL −1 ) showed the highest activity in lymphocytes proliferation assay, but the highest macrophage phagocytic index was shown by M. tuberosa ethanol extract (50 µg mL −1 ). Conclusion: Our study demonstrated that Sarang-Semut tubers are potential to be developed as immunomodulatory agents.

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Section: Discussionmentioning

confidence: 99%

Preliminary Study on Immunomodulatory Effect of Sarang-Semut Tubers Myrmecodia tuberosa and Myrmecodia pendens

Hertiani¹,

Sasmito²,

Sumardi³

et al. 2010

OnLine Journal of Biological Sciences

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“…There are over 4000 compounds that have been characterized as flavonoids by scientists (Syamsudin et al, 2009;Lentini et al, 2007). Nevertheless, this number is just the tip of the iceberg as scientists nowadays are confident of discovering many more of this class of compounds in the plant kingdom in future.…”

Section: Introductionmentioning

confidence: 99%

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)

Tan¹,

Musa²,

Ariff³

et al. 2010

American Journal of Biochemistry and Biotechnology

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Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L −1 sucrose supplemented with different concentrations (0.5-2.5 mg L ). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture

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Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

Ribeiro

Arruda

Mejía

et al. 2021

Chemistry & Biodiversity

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Seven phenolic compounds (ferulic acid, caffeic acid, 4‐methoxycinnamic acid, 3,4‐dimethoxycinnamic acid, 3‐hydroxy‐4‐methoxybenzaldehyde, 3‐methoxy‐4‐hydroxypropiophenone and 1‐O,2‐O‐digalloyl‐6‐O‐trans‐p‐coumaroyl‐β‐D‐glucopyranoside), a flavanonol (7‐O‐methylaromadendrin), two lignans (pinoresinol and matairesinol) and six diterpenic acids/alcohol (19‐acetoxy‐13‐hydroxyabda‐8(17),14‐diene, totarol, 7‐oxodehydroabietic acid, dehydroabietic acid, communic acid and isopimaric acid) were isolated from the hydroalcoholic extract of a Brazilian Brown Propolis and characterized by NMR spectral data analysis. The volatile fraction of brown propolis was characterized by CG‐MS, composed mainly of monoterpenes and sesquiterpenes, being the major α‐pinene (18.4 %) and β‐pinene (10.3 %). This propolis chemical profile indicates that Pinus spp., Eucalyptus spp. and Araucaria angustifolia might be its primary plants source. The brown propolis displayed significant activity against Plasmodium falciparum D6 and W2 strains with IC50 of 5.3 and 9.7 μg/mL, respectively. The volatile fraction was also active with IC50 of 22.5 and 41.8 μg/mL, respectively. Among the compounds, 1‐O,2‐O‐digalloyl‐6‐O‐trans‐p‐coumaroyl‐β‐D‐glucopyranoside showed IC50 of 3.1 and 1.0 μg/mL against D6 and W2 strains, respectively, while communic acid showed an IC50 of 4.0 μg/mL against W2 strain. Cytotoxicity was determined on four tumor cell lines (SK‐MEL, KB, BT‐549, and SK‐OV‐3) and two normal renal cell lines (LLC‐PK1 and VERO). Matairesinol, 7‐O‐methylaromadendrin, and isopimaric acid showed an IC50 range of 1.8–0.78 μg/mL, 7.3–100 μg/mL, and 17–18 μg/mL, respectively, against the tumor cell lines but they were not cytotoxic against normal cell lines. The crude extract of brown propolis displayed antimicrobial activity against C. neoformans, methicillin‐resistant Staphylococcus aureus, and P. aeruginosa at 29.9 μg/mL, 178.9 μg/mL, and 160.7 μg/mL, respectively. The volatile fraction inhibited the growth of C. neoformans at 53.0 μg/mL. The compounds 3‐hydroxy‐4‐methoxybenzaldehyde, 3‐methoxy‐4‐hydroxypropiophenone and 7‐oxodehydroabietic acid were active against C. neoformans, and caffeic and communic acids were active against methicillin‐resistant Staphylococcus aureus.

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Immunomodulatory and In vivo Antiplasmodial Activities of Propolis Extracts

Cited by 12 publications

References 14 publications

Preliminary Study on Immunomodulatory Effect of Sarang-Semut Tubers <i>Myrmecodia tuberosa</i> and <i>Myrmecodia pendens</i>

Preliminary Study on Immunomodulatory Effect of Sarang-Semut Tubers <i>Myrmecodia tuberosa</i> and <i>Myrmecodia pendens</i>

Effect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (<i>centella asiatica</i> L. urban)

Phytochemical, Antiplasmodial, Cytotoxic and Antimicrobial Evaluation of a Southeast Brazilian Brown Propolis Produced by Apis mellifera Bees

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