Immunomodulatory, cytotoxicity, and antioxidant activities of roots of Ziziphus mauritiana

Abstract

Aims:The study is conducted to evaluate the immunomodulatory, cytotoxicity, and antioxidant potential of Ziziphus mauritiana (Rhamnaceae). Phytochemical analysis of Z. mauritiana revealed the presence of alkaloids, anthraquinone glycoside, cardiac glycoside, saponin, tannin, and flavonoids.Methodology:The cytotoxicity of the plant Z. mauritiana was evaluated by brine shrimp lethality test. Antioxidant parameters such as superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) levels were calculated in the plasma of rats after chronic administration of 400 mg/kg of Z. mauritiana for 6 weeks.Results:The dichloromethane extract of the plant exhibited significant immunomodulatory activity, with inhibitory concentration 50% of 55.43 ± 7.9. The dichloromethane extracts of the plant showed 70% mortality at concentration 1000 μg/ml. SOD and T-AOC levels were increased while MDA level in the plasma was reduced in the plasma of rats treated with dichloromethane Z. mauritiana.Conclusion:This can be deduced that the root of Z. mauritiana has immunomodulatory, cytotoxic, and antioxidant potential.SUMMARY Roots of Z. mauritiana was exhibited immunomodulator, cytotoxic and antioxidant activitiesZ. mauritiana showed potential antioxidant activity in rats Abbreviations used: SOD: Superoxide dismutase; T-AOC: Total antioxidant capacity; MDA: Malondialdehyde; ZMRD: Z. mauritiana root extract of dichloromethane fraction; LD50: Z. mauritiana root extract of methanol fraction ZMRM, lethal dose 50.

Wound Healing and Antioxidant Capabilities of Zizyphus mauritiana Fruits: In-Vitro, In-Vivo, and Molecular Modeling Study

“…Group III [MEBOÒ] “1C”: showing collagen bundles packing the defect in a reticular pattern resembling that of the adjacent normal dermis (star) using Masson trichrome stain × 400, Figure S2: Histogram of wounded skin 14 days after incision; Group I [untreated] “2A”: showing the wide wound area, heavy inflammatory cellular infiltration and compact irregular collagen bundles (star); Group II [Z. mauritiana fruits extract] “2B”: showing typical stratified squamous keratinized epithelium and dermal matrix with coarse wavy collagen bundles in different directions (stars), and the newly formed hair follicles; Group III [MEBOÒ] “2C”: showing typical epithelium, thin scar tissue extending into the dermis, reticular dermis has coarse wavy collagen bundles arranged in different directions using Masson trichrome stain × 400, Table S1: The primer sequences of studied genes, Table S2. Dereplicated identified compounds from Zizyphus mauritiana fruits extract [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 68 , 69 , 70 , 71 ].…”

Evaluation de la qualité botanique, physicochimique, phytochimique et de l’activité anti-radicalaire de la pulpe de fruits des échantillons de Ziziphus mauritiana Lam (Rhamnaceae), récoltés au Mali

“…D'autres études ont prouvé l'activité antioxydante des fruits de Ziziphus mauritiana par la réduction du radical DPPH (Okala et al, 2014) et par la réduction de l'ion ferreux. Afzal et al ont trouvé une activité antioxydante avec les racines de Ziziphus mauritiana (Afzal et al, 2017). Le stress oxydant intervient dans la malnutrition par une diminution des facteurs de protection antioxydante (Houssaini et al, 1997) et une détérioration de la flore digestive (Million et al, 2016).…”

A panoramic view on phytochemical, nutritional, and therapeutic attributes of Ziziphus mauritiana Lam.: A comprehensive review

“…SOD and T‐AOC levels were increased while the MDA level in the plasma was reduced in the plasma of rats treated with dichloromethane Z. mauritiana . Thus roots of Z. mauritiana were exhibited promising immunomodulatory activity (Afzal et al, 2017).…”