Immunomodulatory effect of diethylcarbamazine citrate plus filarial excretory–secretory product on rat hepatocarcinogenesis

Abdel‐Latif, Mahmoud; Sakran, Thabet; El-Shahawi, Gamal; El-Fayoumi, Hoda; El-Mallah, Al-Mahy

doi:10.1016/j.intimp.2014.12.004

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…Interferon γ (IFN-γ) was determined by ELISA quantitative sandwich immunoassay kit provided from the Eagle Biosciences, Korea [17]. Interleukin 4 and 5 (IL-4 and IL-5) were determined using a highly sensitive fluorescence microsphere system (Bio-Plex suspension array system, Hercules, CA, USA) [18].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

Abdel-Messeih

Nosseir

Bakhe

2017

cejoi

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Prostate cancer is the second leading cause of cancer-related death in men. The present study was carried out to investigate the radiation response of serum cytokines and oxidative markers to find out if these novel biomarkers have significant applications regarding radiation outcome in prostate cancer patients. Significant elevations of prostatic specific antigen (PSA), asymmetric dimethyl arginine (ADMA) and nitric oxide (NO) were recorded in cancer prostate patients at the time of diagnosis compared to controls. Patients were subjected to radiotherapy post prostatectomy with a total dose of 66 Gy in 33 fractions (5 sessions/week) for 7 weeks. At the end of the seventh week post radiotherapy, ADMA levels were accentuated while the levels of PSA and NO were lower than before therapy. The level of inflammatory cytokines (interleukins IL-4, IL-5 and interferon-gamma) in post radiation therapy patients were significantly elevated compared to both controls and prostate cancer patients. A significant inverse correlation was observed in prostate cancer patients between ADMA and NO. Moreover, a significant inverse correlation in post radiation therapy patients was observed between IL-5 and PSA. These results are highly suggestive that there is a specific cytokine response in patients undergoing curative radiotherapy for prostate cancer.

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Section: Methodsmentioning

confidence: 99%

Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

Abdel-Messeih

Nosseir

Bakhe

2017

cejoi

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“…Furthermore, filaria-derived eicosanoids may participate in host-parasite interactions and modulate the immune response to the parasite ( Kennedy et al., 1997 ; Mpagi et al., 2000b ; Abdel-Latif et al., 2015 ). Filarial eicosanoids such as prostaglandins enhance the Th2 and down-regulate the Th1 type immune responses and are thought to regulate physiological processes enabling the parasites to disseminate and reproduce in the host ( Kennedy et al., 1997 ; Mpagi et al., 2000b ; Abdel-Latif et al., 2015 ).…”

Section: Resultsmentioning

confidence: 99%

The Secretome of Filarial Nematodes and Its Role in Host-Parasite Interactions and Pathogenicity in Onchocerciasis-Associated Epilepsy

Hotterbeekx

Perneel

Vieri

et al. 2021

Front. Cell. Infect. Microbiol.

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Filarial nematodes secrete bioactive molecules which are of interest as potential mediators for manipulating host biology, as they are readily available at the host-parasite interface. The adult parasites can survive for years in the mammalian host, due to their successful modulation of the host immune system and most of these immunomodulatory strategies are based on soluble mediators excreted by the parasite. The secretome of filarial nematodes is a key player in both infection and pathology, making them an interesting target for further investigation. This review summarises the current knowledge regarding the components of the excretory-secretory products (ESPs) of filarial parasites and their bioactive functions in the human host. In addition, the pathogenic potential of the identified components, which are mostly proteins, in the pathophysiology of onchocerciasis-associated epilepsy is discussed.

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“…Previously, repeated doses of SeqES in experimentally induced hepatocarcinogenesis could show some of the protective effects as a result of reduced inflammatory responses and oxidative stress . One of those anti‐inflammatory effects was the inhibition of complement activation.…”

Section: Discussionmentioning

confidence: 99%

“…The anti‐inflammatory properties of ES products could be applied for protection against inflammatory diseases like rheumatoid arthritis . Our previous studies for the SeqES immunomodulatory effect indicated downregulation of macrophage activity, IFN‐γ production by peripheral blood mononuclear cells (PBMCs) and complement activation (CH 50 ) . This, in turn, indicated the existence of complement‐binding or inhibitory protein.…”

Section: Introductionmentioning

confidence: 99%

Identification of the complement 9‐binding protein in Setaria equina excretory‐secretory products

Abdel‐Latif

2019

Parasite Immunology

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The current study aimed to detect the complement-binding proteins in the excretory-secretory (ES) products of adult filarial parasite Setaria equina (SeqES). Tests for complement activation pathways (CH 50 and APH 50 ) in normal human serum (NHS) after incubation with SeqES were performed. Quantitative detection of complement activation products like C3d and sC5b-9 by ELISA in inulin-activated NHS before and after addition of SeqES was estimated. Immunoblotting for 1D and 2D electrophoresed SeqES were performed for detection of C9-binding protein. MALDI mass sequencing and multiple sequence alignment were performed for identification of the protein. The results showed an inhibitory effect of SeqES for complement activation pathways. This was confirmed by an obvious reduction in C3d and sC5b-9 in inulin-activated NHS. Immunoblotting showed the reaction of a protein at 21 kDa with human C9. The latter protein was identified as OV-16 based on MALDI mass sequencing and multiple sequence alignment. In conclusion, S equina OV-16 is the complement regulatory protein by its ability to bind C9 and inhibit the classical and alternative pathways of complement activation. This protein can be used as a target for therapeutic treatment or as an anti-inflammatory agent in human diseases. K E Y W O R D SComplement 9, excretory-secretory products, OV-16, Setaria equina

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Immunomodulatory effect of diethylcarbamazine citrate plus filarial excretory–secretory product on rat hepatocarcinogenesis

Cited by 8 publications

References 45 publications

Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

Evaluation of inflammatory cytokines and oxidative stress markers in prostate cancer patients undergoing curative radiotherapy

The Secretome of Filarial Nematodes and Its Role in Host-Parasite Interactions and Pathogenicity in Onchocerciasis-Associated Epilepsy

Identification of the complement 9‐binding protein in Setaria equina excretory‐secretory products

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