Immunomodulatory effect of protease hydrolysates from ovotransferrin

Liu, Lin; Xu, Mingsheng; Tu, Yonggang; Du, Hongwu; Zhou, Yingqun; Zhu, Gaoxiang

doi:10.1039/c6fo01669c

Cited by 15 publications

(10 citation statements)

References 38 publications

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“…[ 19 ] Scientists have shown that OVT and its hydrolysates exerted an immunomodulatory effect on the maturation of dendritic cells and alleviated immune dysfunction. [ 3,20 ] Our results were in line with the previous literature suggesting the supplement of OVT might contribute to immunomodulatory function in the intestine. Moreover, OVT affected the signal transduction pathway by downregulating the sonic hedgehog (Shh) gene.…”

Section: Resultssupporting

confidence: 91%

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

Zhang

Tan

Yang

et al. 2020

Molecular Nutrition Food Res

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Scope: Egg ovotransferrin (OVT) is considered a functional food ingredient for its various bioactivities. The objective of this work is to explore the potential biological activity of OVT on the gut health. Methods and results: Both young (3 week old) and adult (8 week old) mouse models are utilized in this research. Each group receives a standard diet containing either OVT (experimental group) or distilled water (control group) for a 14 day period. Transcriptome and 16S rDNA sequencing analyses are applied to characterize the gene expression in colonic epithelial cells and gut microbiota composition. In the young groups, OVT suppresses the genes correlated with lipid metabolism and signal transduction. The regulated genes in the adult groups encompass various biological processes, including lipid metabolism, signal transduction, endocrine system, and others. OVT increases the proportion of some beneficial bacteria significantly, especially Akkermansia, and inhibits some harmful bacteria. Furthermore, OVT affects mucosal morphology positively via increasing the crypt depth. OVT also increases the expression of tight junction protein occludin by 3.0-and 5.2-folds in young and adult groups, respectively. Conclusion: OVT exhibits some beneficial effects on the gut environment. These positive findings provide new insight into the understanding of OVT as an excellent functional ingredient.

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Section: Resultssupporting

confidence: 91%

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

Zhang

Tan

Yang

et al. 2020

Molecular Nutrition Food Res

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show abstract

“…But, the biological significance of the potential immunomodulatory activity of egg white pleiotrophin in human intestine remains very speculative. In contrast, some valuable immunomodulatory activities might emerge from ovotransferrin and egg yolk vitellogenin hydrolysates [121,122] after partial degradation by digestive proteases.…”

Section: Egg Nutraceuticalsmentioning

confidence: 99%

The Golden Egg: Nutritional Value, Bioactivities, and Emerging Benefits for Human Health

2019

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Egg is an encapsulated source of macro and micronutrients that meet all requirements to support embryonic development until hatching. The perfect balance and diversity in its nutrients along with its high digestibility and its affordable price has put the egg in the spotlight as a basic food for humans. However, egg still has to face many years of nutritionist recommendations aiming at restricting egg consumption to limit cardiovascular diseases incidence. Most experimental, clinical, and epidemiologic studies concluded that there was no evidence of a correlation between dietary cholesterol brought by eggs and an increase in plasma total-cholesterol. Egg remains a food product of high nutritional quality for adults including elderly people and children and is extensively consumed worldwide. In parallel, there is compelling evidence that egg also contains many and still-unexplored bioactive compounds, which may be of high interest in preventing/curing diseases. This review will give an overview of (1) the main nutritional characteristics of chicken egg, (2) emerging data related to egg bioactive compounds, and (3) some factors affecting egg composition including a comparison of nutritional value between eggs from various domestic species.

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“…In addition, several other groups have described that hydrolysates from different protein sources and different enzymatic preparations stimulate the production of cytokines in basal conditions and exert anti‐inflammatory effects in LPS‑ or ConA‐stimulated cells. The involvement of TLRs and NF κ B and a role of MAPKs has also been described. Considering that the different protein hydrolysates assayed feature unique sequences and specific structural characteristics, such as glycosylation, it is possible that a rather nonspecific mechanism of action is at play.…”

Section: Discussionmentioning

confidence: 99%

Molecular action mechanism of anti‐inflammatory hydrolysates obtained from brewers' spent grain

et al. 2020

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BACKGROUND Brewers’ spent grain (BSG) is a relevant, protein‐rich by‐product of the brewing process. Protein hydrolysates from different sources exert immune‐regulatory actions activating toll‐like receptors (TLRs), nuclear factor kappa B (NFκB), and mitogen‐activated protein kinases (MAPKs). Effects of gastrointestinal digestion have been poorly studied. Here, we studied the immune‐regulatory effect of BSG hydrolysates, and their in‐vitro‐digested products, on rat splenocytes, macrophages, and T lymphocytes RESULTS In primary cultures of rat spleen cells, BSG hydrolysates induced interleukin 10 and tumor necrosis factor production in basal conditions. Under stimulation with lipopolysaccharide or concanavalin A, hydrolysates further induced interleukin 10 production. Tumor necrosis factor and interferon‐γ were inhibited in lipopolysaccharide‑ and concanavalin‐A‐stimulated cells respectively. In vitro gastrointestinal digestion attenuated the observed effects. Splenic macrophages and T lymphocytes behaved in a similar fashion. In spleen cells from TLR2−/− and TLR4−/− mice, immune‐regulatory effects were greatly reduced or abrogated. The study of signal transduction pathways indicated a major involvement of NFκB, and the contribution of MAPKs p38, c‐Jun N‐terminal kinase, and extracellular signal‐regulated kinases 1 and 2. CONCLUSION BSG hydrolysates, like those obtained from other food sources, regulate the immune response, involving TLR2 and TLR4 and the activation of NFκB and MAPKs, an effect partly maintained after in vitro gastrointestinal digestion. Our data support the hypothesis of a shared, rather unspecific, mechanism of action of protein hydrolysates. © 2020 Society of Chemical Industry

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Immunomodulatory effect of protease hydrolysates from ovotransferrin

Cited by 15 publications

References 38 publications

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

Multi‐Omics Analysis of the Effects of Egg Ovotransferrin on the Gut Environment in Mice: Mucosal Gene Expression, Microbiota Composition, and Intestinal Structural Homeostasis

The Golden Egg: Nutritional Value, Bioactivities, and Emerging Benefits for Human Health

Molecular action mechanism of anti‐inflammatory hydrolysates obtained from brewers' spent grain

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