Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward Streptococcus agalactiae Infection

Disbanchong, Purichaya; Punmanee, Wichayaporn; Srithanasuwan, Anyaphat; Pangprasit, Noppason; Wongsawan, Kanruethai; Suriyasathaporn, Witaya; Chuammitri, Phongsakorn

doi:10.3390/ani11113286

Cited by 8 publications

(28 citation statements)

References 31 publications

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“…Alternatively, though, phagocytosis and related processes may just be downstream of the immune activation we observe in all high-intervention groups. Correspondingly, in the context of bovine mastitis, quercetin was shown to enhance phagocytosis and bacterial killing (Disbanchong et al, 2021) and it promotes the expression of genes involved in phagocytosis in bovine neutrophils (Srikok, Nambut, Wongsawan, & Shuammitri, 2017). Overall, the gene expression data align with the inflammation marker data, adding mitochondrial and immune aspects, and they suggest apoptosis and related processes triggered in the highest intervention group, possibly due to the senolysis of senescent blood cells.…”

Section: A Strawberry-based Intervention Improves Mitochondrial Immun...mentioning

confidence: 52%

High-dose Polyphenol-rich Nutrition Improves Lipid and Inflammation Profiles and Triggers Apoptotic Signaling in Healthy Elderly People (the ErdBEHR Study)

Hartmann

Secci

Mensch

et al. 2023

Preprint

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Nutritional interventions in healthy individuals may be particularly informative if high, but not excessive, amounts of specific healthy foods are taken to maximize effects without sacrificing safety. We hypothesized that high amounts of polyphenols taken on single days may eliminate senescent blood cells. We conducted a ten-week parallel-group controlled randomized open trial with an escalation of consumption, up to ~4kg fresh strawberries weekly, plus 200g dried strawberries and 240g capers in olive oil on three single "seno-intervention" days, in 168 healthy elderly people aged 50-80 years. Two primary endpoints, LDL cholesterol and high sensitive CRP, were prespecified. We found a significant decline in LDL cholesterol, and in CRP by ~50% in all groups with seno-intervention days (limited to participants with increased baseline values). LDL levels were reduced by 0.0174 mmol/L for any single 500g-increment in the weekly fresh strawberry intake of the average participant. Gene expression analyses of whole blood suggested improvement of mitochondrial and immunological function, suppression of inflammation (in high-intervention groups), and positive regulation of apoptotic signaling (in the highest-intervention group). Overall, a medium-term nutritional intervention improved lipid and inflammation status, and provided specific hints for apoptotic/senolytic effects.

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Section: A Strawberry-based Intervention Improves Mitochondrial Immun...mentioning

confidence: 52%

High-dose Polyphenol-rich Nutrition Improves Lipid and Inflammation Profiles and Triggers Apoptotic Signaling in Healthy Elderly People (the ErdBEHR Study)

Hartmann

Secci

Mensch

et al. 2023

Preprint

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“…In addition, further research showed that CUR has good antibacterial effects, which may be closely related to the reduced aggregation of FtsZ raw wire and the interrupted cell division, thereby inhibiting the proliferation of bacteria. Moreover, CUR can also induce bacterial apoptosis to express a sterilization effect [ 59 , 60 ]. A previous study demonstrated that CUR could increase the apoptosis protein RECA expression in E. coli , leading to ROS accumulation, membrane exfoliating and calcium ions, and DNA fragments, and eventually induced the bacterial apoptosis [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Current Development and Future Application Prospects of Plants-Derived Polyphenol Bioactive Substance Curcumin as a Novel Feed Additive in Livestock and Poultry

Pan

Yan

Chen

et al. 2022

IJMS

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Curcumin (CUR) is a kind of natural orange-yellow phenolic compound mainly extracted from the stems and roots of turmeric plants and other species in the genus Curcuma, furthermore, it is also the most important active ingredient exerting pharmacological functions in turmeric. In recent years, CUR has been frequently reported and has attracted widespread attention from scholars all over the world due to its numerous biological functions and good application prospects, such as anti-inflammatory, anticancer, antioxidant and providing lipid-lowering effects, etc. In addition, adding a certain dose of CUR to livestock and poultry feed is important for animal growth and development, which plays a key role in animal metabolism, reproduction, immunity and clinical health care. This review aims to summarize, based on the published papers and our own observations, the physical and chemical properties and the biological functions of the plant-derived bioactive ingredient CUR, especially regarding the latest research progress in regulating intestinal health as well as its current development and future application prospects in livestock and poultry as a novel feed additive, so as to provide theoretical and practical references for the further study of the application of CUR as a novel feed additive and a potential new antibiotic substitute, thereby improving the research field of plant-derived bioactive ingredients and promoting the healthy development of livestock and poultry.

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“…Pellets were rinsed twice with cold Hanks’ Balanced Salt Solution (HBSS) and resuspended in cold Roswell Park Memorial Institute 1640 (RPMI 1640) medium containing 1% heat-inactivated foetal bovine serum (FBS, Gibco, Thermo Fisher Scientific, Waltham, MA, USA). The Trypan blue exclusion method and cytospin were used to assess the viability and to determine the neutrophil purity, respectively [ 19 ]. The results show a viability rate of over 95%, while the purity of the cells was more than 85%.…”

Section: Methodsmentioning

confidence: 99%

“…As major causes of bovine mastitis, Staphylococcus aureus (SAU), Streptococcus agalactiae (SAG) [ 19 ], and Streptococcus uberis (SUB) [ 27 ] were selected for the current study. Bovine non-aureus staphylococci and mammaliicocci (NASM); Staphylococcus hominis (SHO) and Staphylococcus chromogenes (SCH); lactic acid bacteria (LAB); and two isolates of Weissella paramesenteroides (WPA1 and WPA2) with antimicrobial activity against S. aureus or Escherichia coli were used as minor pathogen-causing bovine mastitis in the entire experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Live bacteria from Section 2.2 were grown to the log phase, suspended in a Hanks’ balanced salt solution (HBSS) solution (Sigma-Aldrich, St. Louis, MO, USA), and heat-killed at 70°C for 60 min [ 19 , 28 ]. Heat-killed bacteria were resuspended at a 10 8 CFU/mL density in 1 µg/mL goat anti-mouse IgG (H+L) antibody, Alexa Fluor ® 488 (Invitrogen, Thermo Fisher Scientific) for SAU, SAG, and SCH.…”

Section: Methodsmentioning

confidence: 99%

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Exploring the distinct immunological reactions of bovine neutrophils towards major and minor pathogens responsible for mastitis

Srithanasuwan,

Tata,

Tananupak

et al. 2023

International Journal of Veterinary Science and Medicine

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Bovine mastitis is primarily caused by a group of bacteria known as Staphylococcus and Streptococcus . However, additional types of bacteria, such as bovine non-aureus staphylococci and mammaliicocci (NASM) as well as lactic acid bacteria (LAB), are considered minor pathogens and have less impact on cows. Modulating bovine neutrophil activities and gene expressions in response to bacterial stimuli prompted the cells to execute effector functions to combat udder infections. Although neutrophils can manage major mastitis-causing bacteria, this strategy has not been tested against minor pathogens, i.e. NASM, Weissella spp. Our main objective was to investigate how neutrophils interacted with major and minor pathogens during in vitro bacterial stimulation. The results reveal that neutrophils performed offensive duties regardless of the type of bacteria encountered. Neutrophils generated high levels of reactive oxygen species, efficiently phagocytosed both types of bacteria, and facilitated extracellular killing by releasing NET structures against all bacteria. In addition, neutrophils migrated preferentially towards the majors rather than the minors, although myeloperoxidase (MPO) degranulation did not differ substantially across bacteria. Furthermore, the killing capacity of neutrophils was not dependent on any particular bacterium. The correlation of effector functions is intimately linked to the up-regulation of genes associated with the above functions, except for IL6 , which was down-regulated. Furthermore, neutrophil apoptosis can be modulated by altering apoptosis-associated genes in response to harmful stimuli. These findings provide valuable information on how neutrophils react to major and minor mastitis-causing bacteria. However, future research should explore the interplay between minor pathogens and the host’s responses.

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Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward Streptococcus agalactiae Infection

Cited by 8 publications

References 31 publications

High-dose Polyphenol-rich Nutrition Improves Lipid and Inflammation Profiles and Triggers Apoptotic Signaling in Healthy Elderly People (the ErdBEHR Study)

High-dose Polyphenol-rich Nutrition Improves Lipid and Inflammation Profiles and Triggers Apoptotic Signaling in Healthy Elderly People (the ErdBEHR Study)

Current Development and Future Application Prospects of Plants-Derived Polyphenol Bioactive Substance Curcumin as a Novel Feed Additive in Livestock and Poultry

Exploring the distinct immunological reactions of bovine neutrophils towards major and minor pathogens responsible for mastitis

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