Immunomodulatory effects of<i>Pteridium aquilinum</i>on natural killer cell activity and select aspects of the cellular immune response of mice

Latorre, Andréia Oliveira; Furlan, Maria Stella; Sakai, Mônica; Fukumasu, Heidge; Hueza, Isis Machado; Haraguchi, Mitsue; Górniak, Silvana Lima

doi:10.1080/15476910902972465

Cited by 28 publications

(26 citation statements)

References 30 publications

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“…Previous studies conducted in this laboratory have also demonstrated (in mice) that ptaquiloside produces immunosuppressive effects, including direct toxic effects on NK cells (Latorre et al, 2009). The toxin principally responsible for P. aquilinum-induced carcinogenic and immunosuppressive effects is ptaquiloside, a norsesquiterpene glucoside that acts as a DNA-alkylating agent (Hirono, 1986;Latorre et al, 2009;Yamada et al, 2007).…”

Section: Introductionmentioning

confidence: 85%

“…They were frozen at À80 C until utilized for extract preparation. The extract was prepared as previously described by Latorre et al (2009).…”

Section: Pteridium Aquilinummentioning

confidence: 99%

“…The dose of P. aquilinum extract (30 g/kg/day) used in this experiment was based on a previous study performed by our group (Latorre et al, 2009) and corresponded to 5.3 mg/kg of ptaquiloside as quantified in crosiers collected at the same time and place that we used in another study (Latorre et al, 2011). All mice were supplemented with vitamin B 1 in their drinking water (10 mg/ml; as proposed by Schacham et al, 1970) throughout the treatment period to prevent the effects of thiaminase 1, one toxic component of P. aquilinum (Fenwick, 1988).…”

Section: Treatment Protocolmentioning

confidence: 99%

“…At the end of the 13-week treatment period, sets of control and experimental mice (distinct from those used for the lung evaluation studies; Figure 1A) were euthanized to prepare splenic cell suspensions and non-adherent splenic cell populations as previously described (Latorre et al, 2009). Non-adherent splenic cells were used as effector cells and YAC-1 (ATCC TIB-160, Manassas, VA) cells (mouse lymphoma cell line) as targets in the NK assays.…”

Section: Nk Cell Activity Assaymentioning

confidence: 99%

“…Humans can be exposed to the plant indirectly through consumption of milk and meat from animals grazing on bracken fern (Alonso-Amelot & Avendano, 2002;Fletcher et al, 2011;Shahin et al, 1999) or through ingestion of contaminated water (Rasmussen et al, 2003). Previous studies conducted in this laboratory have also demonstrated (in mice) that ptaquiloside produces immunosuppressive effects, including direct toxic effects on NK cells (Latorre et al, 2009). The toxin principally responsible for P. aquilinum-induced carcinogenic and immunosuppressive effects is ptaquiloside, a norsesquiterpene glucoside that acts as a DNA-alkylating agent (Hirono, 1986;Latorre et al, 2009;Yamada et al, 2007).…”

Section: Introductionmentioning

confidence: 97%

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Immunosuppressive effects ofPteridium aquilinumenhance susceptibility to urethane-induced lung carcinogenesis

Caniceiro¹,

Latorre²,

Fukumasu

et al. 2014

Journal of Immunotoxicology

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Pteridium aquilinum (bracken fern), one of the most important toxic plants in the world, contains the toxic norsequiterpene ptaquiloside that induces cancers in humans and farm animals. Previous studies in the laboratory demonstrated immunotoxic effects produced by ptaquiloside, which are characterized by suppression of natural killer (NK) cell activity (i.e. cytotoxicity and interferon [IFN]-production). However, it is unknown whether these immunosuppressive effects could contribute to carcinogenesis in situ in general because of the important function of NK cells in innate killing of tumor cells. This study assessed the impact of P. aquilinum-induced immunosuppression on urethane-induced lung cancer in C57BL/6 mice. Adult mice were treated with an extract of P. aquilinum (30 g/kg/day) by gavage once daily for 14 days, followed by gavage (5 days/week) during an 11-week period that was accompanied by treatment with urethane (1 g/kg) via once-weekly intraperitoneal injection; 20 weeks after the end of the treatment period, all lungs were evaluated. The results indicated there was a significant increase in lung nodule number as well as in multiplicity of lesions in mice treated with both P. aquilinum and urethane (PU group) compared to values in mice treated only with the urethane (U group). In addition, histologic evaluation revealed a 76% increase in the rate of lung adenomas and a 41% increase in rate of bronchiolization of alveoli in the mice from the PU group compared to levels seen in mice within the U group. Taken together, the results here show for the first time that immunosuppressive effects of P. aquilinum could increase the risk of cancer formation in exposed hosts.

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