Immunomodulatory effects of Ziziphora tenuior L. extract on the dendritic cells

Abstract

BackgroundZiziphora tenuior L. (Kakuti in Persian) is used in traditional medicine for treatment of gastrointestinal disorders as carminative and analgesic plant. The other usages of this plant are included treatment of diarrhea and nausea. Therefore in the present study we evaluated the immunomodulatory effects of the ethanolic extract of this plant on the dendritic cells (DCs).ResultsZiziphora tenuior L. extract significantly (p = 0.002) increased the level of surface expression of CD40 as an important co-stimulatory marker on DCs compared to the control. However this extract did not change CD86 and MHC-II molecules, so it could promote DCs phenotypic maturation. Treatment of DCs with the extract resulted in slightly increased of the production of (IL-12); however, this change was not significant. In addition, the ability of treated DCs to stimulate allogenic T cells proliferation and cytokines secretion was examined in the co-cuture of these cells with T cells in mixed lymphocyte reaction (MLR). Z. tenuior L. at the 100 μg/ml concentration inhibited the proliferation of allogenic T cells and also significantly (P < 0.001) increased the level of IL-10. Moreover, the extract at 10–100 μg/ml concentration caused slightly increase in IFN-γ production and decreased IL-4 cytokines but these changes were not significant.ConclusionsThese findings indicated that Z. tenuior L. extract can modulate immune response by induction of CD40 expression on DCs and cytokine production; whereas it can inhibit T cell stimulating activity of DCs in high concentration. These findings possibly in part explain the traditional use of this plant in treatment of immune-mediated disorders. However future studies are needed.

Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review

“…The antimicrobial activity of the essential oil of Salmonella typhi Vi-positive makes it useful in the treatment of typhoid fever, too. The plant extract can modulate immune response by induction of CD40 expression on DCs and cytokine production and inhibition of T-cell stimulating activity of dendritic cells in high concentration [ 268 ].…”

Modulation of dendritic cell immune functions by plant components

“…Recently, Gold Lotion, a formulated product prepared from the peels of six citrus fruits, displayed immunomodulatory effect on LPS-stimulated mouse BMDC maturation and function by significantly decreasing production of proinflammatory cytokines and chemokines, inhibiting expression of maturation markers, increasing phagocytic ability, and reducing the propensity to stimulate autologous CD4+ and CD8+ T-cell proliferation [ 33 ]. Ziziphora tenuior L. (Kakuti in Persian) a traditional medicine for treatment of gastrointestinal disorders was found to stimulate CD40 expression on DCs and cytokine production at low concentrations; however, it can prevent T-cell stimulation of DCs at high concentrations [ 34 ]. Likewise, extract of Chrysanthemum coronarium L. induces DC maturation and production of IL-12 [ 35 ].…”

Kazakh Ziziphora Species as Sources of Bioactive Substances

“…Benzylalcohol glucoside (23) Z. clinopodioides [4] Weak inhibitory activity on NO production stimulated by LPS and IFN-γ in RAW 264.7 cells [4] Antihypertensive effect [120] Low anti-inflammatory activity [121] Neuroprotective effect [122] Weak inhibition of TPA-induced EBV-EA activation [123] O Glc Phenethylalcohol glucoside (24) Z. clinopodioides [4] Weak inhibitory activity on NO production stimulated by LPS and IFN-γ in RAW 264.7 cells [4] Neuroprotective effect [122] Antiradical activity, weak ACE-inhibitory activity [124] Inhibition of osteoclast differentiation [125] O Glc Eugenol (25) Z. tenuior [94] Insecticidal (against L. serricorne) [126] Acaricidal activity [127] Antibacterial [128] Inhibition of tyrosine kinase [129] Review on possible antidepressive activity [130] Review on antibacterial effect against cariogenic bacteria [131] Review on possible synergy of eugenol containing essential oils and eugenol (25) with antibiotics [132] Review on antioxidative effect [133] General properties reviewed here [134] OH OMe Eugenol (25) Z. tenuior [94] Insecticidal (against L. serricorne) [126] Acaricidal activity [127] Antibacterial [128] Inhibition of tyrosine kinase [129] Review on possible antidepressive activity [130] Review on antibacterial effect against cariogenic bacteria [131] Review on possible synergy of eugenol containing essential oils and eugenol (25) with antibiotics [132] Review on antioxidative effect [133] General properties reviewed here [134] Molecules 2016, 21, 826 8 of 53…”

“…Phenethylalcohol glucoside (24) Z. clinopodioides [4] Weak inhibitory activity on NO production stimulated by LPS and IFN-γ in RAW 264.7 cells [4] Neuroprotective effect [122] Antiradical activity, weak ACE-inhibitory activity [124] Inhibition of osteoclast differentiation [125] O Glc Eugenol (25) Z. tenuior [94] Insecticidal (against L. serricorne) [126] Acaricidal activity [127] Antibacterial [128] Inhibition of tyrosine kinase [129] Review on possible antidepressive activity [130] Review on antibacterial effect against cariogenic bacteria [131] Review on possible synergy of eugenol containing essential oils and eugenol (25) with antibiotics [132] Review on antioxidative effect [133] General properties reviewed here [134] OH OMe Phenethylalcohol glucoside (24) Z. clinopodioides [4] Weak inhibitory activity on NO production stimulated by LPS and IFN-γ in RAW 264.7 cells [4] Neuroprotective effect [122] Antiradical activity, weak ACE-inhibitory activity [124] Inhibition of osteoclast differentiation [125] Molecules 2016, 21, 826 8 of 53…”