Immunomodulatory properties of human recombinant lactoferrin in mice: Implications for therapeutic use in humans

Artym, Jolanta; Kocięba, Maja; Zaczyńska, Ewa; Adamik, Barbara; Kübler, Andrzej; Zimecki, Michał; Kruzel, Marian L.

doi:10.17219/acem/68440

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…Based on in vitro experiments, we studied the effects of LF on the construction of an animal model of P. acnes . Several mechanisms have been proposed to explain the anti‐inflammatory actions of LFs in mouse models, toll‐like receptors (TLRs), in particular TLR4, could be a good candidate for the mediation of the protective action of LF in this experimental model since LF down‐regulate the function of these receptors 13 . Studies have reported that the injection of active P. acnes can be used to construct animal acne models.…”

Section: Discussionmentioning

confidence: 99%

“…And it has been shown that lactoferrin enters cells through receptor-mediated phosphorylation of P65 protein, which ultimately activates the NF-κB signaling pathway for anti-inflammatory regulation12 we included that LF inhibits the acne inflammatory response via the TLR-2/NF-κB signaling pathway.Based on in vitro experiments, we studied the effects of LF on the construction of an animal model of P. acnes. Several mechanisms have been proposed to explain the anti-inflammatory actions of LFs in mouse models, toll-like receptors (TLRs), in particular TLR4, could be a good candidate for the mediation of the protective action of LF in this experimental model since LF down-regulate the function of these receptors 13. Studies have reported that the injection of active P. acnes can be used to construct animal acne models.…”

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confidence: 99%

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Influence of lactoferrin on Propionibacterium acnes ‐induced inflammation in vitro and in vivo

Cui

Wei

2020

Dermatologic Therapy

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Lactoferrin (LF) is a monomer glycoprotein in the mammalian colostrum that has multiple biological activities and a high affinity for iron ions. Not only does it have strong antibacterial activity, it also can regulate the release of cytokines in inflammatory areas, activate immune cells, and inhibit inflammatory diseases caused by nonpathogenic bacteria and the development of tumors. However, the anti-inflammatory mechanism of LF in inflammatory skin diseases induced by Propionibacterium acnes (P. acnes) has not been elucidated in vitro and in vivo. In this study, we investigated the effects of LF on the generation of inflammatory cytokines in HaCaT cells induced by heat-killed P. acnes. The expression of pro-inflammatory cytokines IL-8 increased after induction of HaCaT by heat-killed P. acnes, but it decreased significantly after LF treatment. Western blotting was used to examine the levels of TLR2, nuclear factor (NF) κB and intercellular cell adhesion molecule 1 protein induced by P. acnes in HaCaT cells, and the results showed that the levels were inhibited by LF. In addition, activated P. acnes (1 × 107 CFU/mL) was injected into the ears of experimental mice, which induced inflammation 24 hours after the injection. However, immunohistochemical analysis showed a significant reduction in the inflammatory response after LF treatment in the right ear relative to the untreated left ear, and the same result was seen with western blotting. In summary, this study revealed the treatment effect of LF on P. acnes-induced inflammation, thus providing support for the anti-acne properties of LF.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Influence of lactoferrin on Propionibacterium acnes ‐induced inflammation in vitro and in vivo

Cui

Wei

2020

Dermatologic Therapy

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“…Since Lf binds to iron, its absence is also associated with a simultaneous interruption of bacterial growth, which prevents infection of the body [ 27 ]. In addition, it has an immunomodulatory effect, accelerating the development of immune system cells and stimulating the body to produce chemokines and cytokines ( Figure 1 ) [ 28 , 29 , 30 , 31 ]. In addition, the bacterial cell membrane is damaged, or its metabolism is altered.…”

Section: Antibacterial Components Of Milk/colostrummentioning

confidence: 99%

Antimicrobial Properties of Colostrum and Milk

Eker,

Akdaşçi,

Duman

et al. 2024

Antibiotics

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The growing number of antibiotic resistance genes is putting a strain on the ecosystem and harming human health. In addition, consumers have developed a cautious attitude towards chemical preservatives. Colostrum and milk are excellent sources of antibacterial components that help to strengthen the immunity of the offspring and accelerate the maturation of the immune system. It is possible to study these important defenses of milk and colostrum, such as lactoferrin, lysozyme, immunoglobulins, oligosaccharides, etc., as biotherapeutic agents for the prevention and treatment of numerous infections caused by microbes. Each of these components has different mechanisms and interactions in various places. The compound’s mechanisms of action determine where the antibacterial activity appears. The activation of the antibacterial activity of milk and colostrum compounds can start in the infant’s mouth during lactation and continue in the gastrointestinal regions. These antibacterial properties possess potential for therapeutic uses. In order to discover new perspectives and methods for the treatment of bacterial infections, additional investigations of the mechanisms of action and potential complexes are required.

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A cytotoxic effect of human lactoferrin fusion with Fc domain of IgG

et al. 2022

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Immunomodulatory properties of human recombinant lactoferrin in mice: Implications for therapeutic use in humans

Abstract: Evidence for the potential preventive or therapeutic utility of rhLF in patients with impaired immune reactivity has been demonstrated.

Cited by 7 publications

References 21 publications

Influence of lactoferrin on Propionibacterium acnes ‐induced inflammation in vitro and in vivo

Influence of lactoferrin on Propionibacterium acnes ‐induced inflammation in vitro and in vivo

Antimicrobial Properties of Colostrum and Milk

A cytotoxic effect of human lactoferrin fusion with Fc domain of IgG

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