Immunomodulatory properties of polysaccharopeptide derived from Coriolus versicolor and its combined effect with Cyclosporine a in activated human T-cells

Abstract

Objectives Lymphocyte homoeostasis is essential in inflammatory and autoimmune diseases. In search of natural fungal metabolites with effects on lymphocyte homoeostasis, we recently reported that polysaccharopeptide (PSP) from Coriolus versicolor exhibited ciclosporin‐like activity in controlling aberrant lymphocyte activation. This object of this study was to investigate its effect on lymphocyte homoeostasis. This was done by investigating the mechanistic actions of PSP in relation to ciclosporin by performing cell cycle and cell death analysis of human lymphocytes in vitro. Methods We investigated the effect of PSP in the presence and absence of ciclosporin on cell proliferation, cell cycle, cell death, immunophenotype and cell cycle regulatory proteins in human lymphocytes. Key findings The data showed that PSP exhibited homoeostatic activity by promoting and inhibiting the proliferation of resting and phytohaemagglutinin (PHA)‐stimulated lymphocytes, respectively. PHA‐stimulated lymphocytes exhibited G0/G1 cell cycle arrest that was accompanied by a reduction of cyclin E expression with PSP treatment. Both PSP and ciclosporin blocked the reduction of the CD4/CD8 ratio in stimulated lymphocytes. PSP did not induce cell death in human lymphocytes, but the suppression of the Fasreceptor suggested a protective role of PSP against extrinsic cell death signals. These homoeostatic effects were more potent with combined PSP and ciclosporin treatment than with either fungal metabolite alone. Conclusions Collectively, the results reveal certain novel effects of PSP in lymphocyte homoeostasis and suggest potential as a specific immunomodulatory adjuvant for clinical applications in the treatment of autoimmune diseases.

Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro

“…Obviously, effects on the ODC and c‐Myc as well as TGF‐ β are important to maintain the continuity of the surface epithelium and rapidly restore it after destruction. Many natural products are known to possess multiple bioactivities [16–21] . We suppose the polysaccharides from Ganoderma lucidum (Gl ‐ PS), which are natural products with multiple actions, may possess the activity to improve the restoration of the continuity of the surface epithelium after destruction.…”

Coriolus versicolor alleviates diabetic cardiomyopathy by inhibiting cardiac fibrosis and NLRP3 inflammasome activation

“…Coriolus versicolor (CV), which belongs to the basidiomycete family, has been a well‐known traditional food and medicinal mushroom for thousands of years. Previous studies have found that CV has antitumor, antioxidant, antibacterial, and immunomodulatory effects on various conditions (Chang et al, ; Fritz et al, ; Lee, Jiang, Sit, Yang, & Wan, ; Saleh, Rashedi, & Keating, ). Recently, CV extract was reported to have efficient hypoglycemic effects.…”

Ganoderma lucidum polysaccharides antagonize the suppression on lymphocytes induced by culture supernatants of B16F10 melanoma cells

“…Krast (Ganodermataceae), a well‐known medicinal mushroom widely used for centuries in China to promote longevity and improve vigour without significant adverse effects [3,4] . As with many natural products with immunomodulatory and antitumour effects, [5–9] Gl ‐PS has been extensively studied in recent decades and demonstrated to have various biological activities, including immunomodulatory and antitumour effects [10] . The immunomodulatory and antitumour effects of Gl ‐PS include: enhancement of cytokine production as well as dendritic cell maturation and function, [11] improvement in cytokine‐induced killer cell function, [12] augmentation of cytotoxic T lymphocyte (CTL) function, [13] promotion of B16F10 cells to activate lymphocytes, [14] and inhibition of the growth of vascular endothelial cells and inhibition of the induction of vascular endothelial growth factor (VEGF) in human lung cancer cell [15] .…”