Effects of <i>Ganoderma lucidum</i> polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing <i>in vitro</i>

Sun, Lixin; Chen, Lihua; Lin, Zhibin; Qin, Ying; Zhang, Jiaqi; Yang, Jing; Ma, Jie; Ye, Ting; Li, Weidong

doi:10.1111/j.2042-7158.2011.01367.x

Cited by 18 publications

(9 citation statements)

References 51 publications

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“…These beneficial features are similar to those with hydrophilic bandage soft contact lenses whose high oxygen permeability is thought to be able to promote epithelial migration and enhance epithelial stromal adhesion [ 29 ]. When corneal epithelial cells were co-cultured with polysaccharide, the rate of cell migration was significantly increased, consistent with previous reports [ 30 ].…”

Section: Discussionsupporting

confidence: 91%

Polysaccharide Hydrogel Combined with Mesenchymal Stem Cells Promotes the Healing of Corneal Alkali Burn in Rats

Cui

et al. 2015

PLoS ONE

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Corneal chemical burns are common ophthalmic injuries that may result in permanent visual impairment. Although significant advances have been achieved on the treatment of such cases, the structural and functional restoration of a chemical burn-injured cornea remains challenging. The applications of polysaccharide hydrogel and subconjunctival injection of mesenchymal stem cells (MSCs) have been reported to promote the healing of corneal wounds. In this study, polysaccharide was extracted from Hardy Orchid and mesenchymal stem cells (MSCs) were derived from Sprague-Dawley rats. Supplementation of the polysaccharide significantly enhanced the migration rate of primarily cultured rat corneal epithelial cells. We examined the therapeutic effects of polysaccharide in conjunction with MSCs application on the healing of corneal alkali burns in rats. Compared with either treatment alone, the combination strategy resulted in significantly better recovery of corneal epithelium and reduction in inflammation, neovascularization and opacity of healed cornea. Polysaccharide and MSCs acted additively to increase the expression of anti-inflammatory cytokine (TGF-β), antiangiogenic cytokine (TSP-1) and decrease those promoting inflammation (TNF-α), chemotaxis (MIP-1α and MCP-1) and angiogenesis (VEGF and MMP-2). This study provided evidence that Hardy Orchid derived polysaccharide and MSCs are safe and effective treatments for corneal alkali burns and that their benefits are additive when used in combination. We concluded that combination therapy with polysaccharide and MSCs is a promising clinical treatment for corneal alkali burns and may be applicable for other types of corneal disorder.

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Section: Discussionsupporting

confidence: 91%

Polysaccharide Hydrogel Combined with Mesenchymal Stem Cells Promotes the Healing of Corneal Alkali Burn in Rats

Cui

et al. 2015

PLoS ONE

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“…The water-soluble polysaccharide fractions of G. lucidum have been reported to have healing effects especially on ulcer lesions [32, 33]. Elsewhere, Sun et al [34] reported that G. lucidum polysaccharides showed healing effects on intestinal epithelium using a nontransformed small-intestinal epithelial cell line, IEC-6 cells. Despite all the beneficial wound healing effects of G. lucidum , little attention has been paid to its effects on wounds associated to diabetes.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides-Rich Extract ofGanoderma lucidum(M.A. Curtis:Fr.) P. Karst Accelerates Wound Healing in Streptozotocin-Induced Diabetic Rats

Cheng

Phan

Sabaratnam

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Ganoderma lucidum (M.A. Curtis:Fr.) P. Karst is a popular medicinal mushroom. Scientific reports had shown that the wound healing effects of G. lucidum were partly attributed to its rich polysaccharides. However, little attention has been paid to its potential effects on wounds associated with diabetes mellitus. In this study, we evaluated the wound healing activity of the hot aqueous extract of G. lucidum in streptozotocin-induced diabetic rats. The extract of G. lucidum was standardised based on chemical contents (w/w) of total polysaccharides (25.1%), ganoderic acid A (0.45%), and adenosine (0.069%). Six groups of six rats were experimentally wounded in the posterior neck region. Intrasite gel was used as a positive control and aqueous cream as the placebo. Topical application with 10% (w/w) of mushroom extract-incorporated aqueous cream was more effective than that with Intrasite gel in terms of wound closure. The antioxidant activity in serum of rats treated with aqueous extract of G. lucidum was significantly higher; whereas the oxidative protein products and lipid damage were lower when compared to those of the controls. These findings strongly support the beneficial effects of standardised aqueous extract of G. lucidum in accelerating wound healing in streptozotocin-induced diabetic rats.

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“…Chrysanthemi flos compounds inhibit NO, PGE-2, TNF- α , and IL-1 β production, as well as iNOS and COX-2 expression in LPS-induced macrophages [ 60 ]. Ganoderma lucidum showed positive effects on intestinal epithelium healing [ 61 ] and refractory diabetic wounds [ 62 ] and was found to decrease NO, PGE-2, and proinflammatory cytokine production, including IL-1 β , TNF- α and NF- κ B in microglia [ 63 ]. Menthae haplocalycis herba in TCM theory is used for mouth sores, exanthema, and itching [ 24 – 26 ].…”

Section: Herbal Combinationsmentioning

confidence: 99%

A Survey of Chinese Medicinal Herbal Treatment for Chemotherapy-Induced Oral Mucositis

Meyer-Hamme

Beckmann

Radtke

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Oral mucositis is one of the common side effects of chemotherapy treatment with potentially severe implications. Despite several treatment approaches by conventional and complementary western medicine, the therapeutic outcome is often not satisfactory. Traditional Chinese Medicine (TCM) offers empirical herbal formulas for the treatment of oral ulceration which are used in adaptation to chemotherapy-induced mucositis. While standard concepts for TCM treatment do not exist and acceptance by conventional oncologists is still low, we conducted a review to examine the evidence of Chinese herbal treatment in oral mucositis. Eighteen relevant studies on 4 single herbs, 2 combinations of 2 herbs, and 11 multiherbal prescriptions involving 3 or more compounds were included. Corresponding molecular mechanisms were investigated. The knowledge about detailed herbal mechanisms, especially in multi-herbal prescriptions is still limited. The quality of clinical trials needs further improvement. Meta-analysis on the existent database is not possible but molecular findings on Chinese medicinal herbs indicate that further research is still promising for the treatment of chemotherapy-induced oral mucositis.

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Effects of Ganoderma lucidum polysaccharides on IEC-6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro

Abstract: These results suggest the potential usefulness of Gl-PS to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.

Cited by 18 publications

References 51 publications

Polysaccharide Hydrogel Combined with Mesenchymal Stem Cells Promotes the Healing of Corneal Alkali Burn in Rats

Polysaccharide Hydrogel Combined with Mesenchymal Stem Cells Promotes the Healing of Corneal Alkali Burn in Rats

Polysaccharides-Rich Extract ofGanoderma lucidum(M.A. Curtis:Fr.) P. Karst Accelerates Wound Healing in Streptozotocin-Induced Diabetic Rats

A Survey of Chinese Medicinal Herbal Treatment for Chemotherapy-Induced Oral Mucositis

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