Immunophenotypic Characterization of Owl Monkey Peripheral Blood Mononuclear Cells^a

Abstract

A two-phase study was initiated to delineate the peripheral blood lymphocyte populations present in owl monkeys and to correlate those populations with immune response and parasitism during malaria infection. The goal of phase I of the study was to elucidate a monoclonal antibody panel that could be used to characterize peripheral blood mononuclear cell (PBMC) populations with flow cytometric techniques. Forty-two monoclonal antibodies (reported to be reactive with human and macaque lymphocyte antigens) were screened for activity to owl monkey PBMC. Eleven monoclonals were found to react: anti-H42A (MHC Class II DP-like); anti-TH14B (MHC Class II DR-like); and anti-TH81A5 (MHC Class II DQ-like); anti-H58A (MHC Class I); anti-DH59B (granulocyte and monocyte); anti-B1 (B cell); anti-T4 (CD4); anti-Leu3a (CD4); anti-Leu11a (CD16); anti-60.3 (CD18); and anti-OKM1 (NK and monocyte). In a preliminary retrospective study correlating antibody titers, parasitemia values, and MHC Class I and Class II marker profiles on PBMC to test antigens used in malaria vaccine trials, a significant negative association was observed between cells bearing MHC Class II molecules and the other elements of the comparison. In summary, an appropriate panel of monoclonal antibodies has been identified for characterizing PBMC in owl monkeys, and preliminary studies indicate a possible association between clinical outcome and expressed phenotypic PBMC markers.

Reactivity of human T‐lymphocyte‐specific antibodies with peripheral blood mononuclear cells and spleen of Aotus azarae ssp. boliviensis (owl monkey)

“…Table 3 summarizes all mAb that have been reported to react with T lymphocytes from Aotus monkeys. Together with the present study, a set of mAb defining a total of six different T lymphocyte antigens in owl monkeys has now been described, although for some mAb contradictory results have been reported [2,18].…”

“…Several mAb against various markers on Aotus PBMC have been reported earlier by others [2,10,[12][13][14]18]. Table 3 summarizes all mAb that have been reported to react with T lymphocytes from Aotus monkeys.…”

“…To obtain optimal results from such studies, a thorough knowledge of the immune system of these New World primates is required. To date, only a limited number of monoclonal antibodies (mAb) to characterise peripheral blood mononuclear cells (PBMC) from owl monkeys has been identified [2,10,[12][13][14]18]. Recently, we reported the reactivity of a large panel of mAb with PBMC from several nonhuman primates, i.e.…”

Proliferative response of peripheral blood lymphocytes to mitogens in the owl monkey Aotus nancymae

“…Several laboratories including ours have carried out extensive studies using these non‐human primate species in an effort to support its use as an experimental malaria model (4, 6, 9, 12–14, 25, 28, 35, 40) and particularly for rational malaria vaccine design (31, 33). However, to date, only a few functional studies for assessing basic immunologic processes in these monkeys have been conducted.…”