Immunophenotyping of T Lymphocytes by Three-Color Flow Cytometry in Healthy Newborns, Children, and Adults

McCloskey, Thomas W.; Cavaliere, Terri A.; Bakshi, Saroj; Harper, Rita G.; Fagin, James; Kohn, Nina; Pahwa, Savita

doi:10.1006/clin.1997.4370

Cited by 54 publications

(50 citation statements)

References 39 publications

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“…The relative numbers of T cells detected here for control PBMC (data not shown) were similar to those observed by McCloskey et al (41) for the normal adult range, while the values of B lymphocytes were similar to those reported by Hamilton (33).…”

Section: Discussionsupporting

confidence: 91%

Immune response to respiratory syncytial virus in young Brazilian children

Queiróz

Durigon

Botosso

et al. 2002

Braz J Med Biol Res

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We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients £12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases ³3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10 5 TCID 50 ) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSVspecific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.

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Section: Discussionsupporting

confidence: 91%

Immune response to respiratory syncytial virus in young Brazilian children

Queiróz

Durigon

Botosso

et al. 2002

Braz J Med Biol Res

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“…As previously shown (32), all PBMC in the infants were CD38 positive. However, at the same time of gaining CCR5 expression, CD8 ϩ T cells in PBMC up-regulated CD38 two to three fold compared with resting T cells.…”

Section: Numbers Of Activated Cd8 ϩ T Cells In Pbmc Of Rsv-infected Isupporting

confidence: 79%

CD8+ T Cell Responses in Bronchoalveolar Lavage Fluid and Peripheral Blood Mononuclear Cells of Infants with Severe Primary Respiratory Syncytial Virus Infections

Heidema

Lukens

Maren

et al. 2007

The Journal of Immunology

106

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A protective role for CD8+ T cells during viral infections is generally accepted, but little is known about how CD8+ T cell responses develop during primary infections in infants, their efficacy, and how memory is established after viral clearance. We studied CD8+ T cell responses in bronchoalveolar lavage (BAL) samples and blood of infants with a severe primary respiratory syncytial virus (RSV) infection. RSV-specific CD8+ T cells with an activated effector cell phenotype: CD27+CD28+CD45RO+CCR7−CD38+HLA-DR+Granzyme B+CD127− could be identified in BAL and blood. A high proportion of these CD8+ T cells proliferated and functionally responded upon in vitro stimulation with RSV Ag. Thus, despite the very young age of the patients, a robust systemic virus-specific CD8+ T cell response was elicited against a localized respiratory infection. RSV-specific T cell numbers as well as the total number of activated effector type CD8+ T cells peaked in blood around day 9–12 after the onset of primary symptoms, i.e., at the time of recovery. The lack of a correlation between RSV-specific T cell numbers and parameters of disease severity make a prominent role in immune pathology unlikely, in contrast the T cells might be involved in the recovery process.

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“…In unaffected infants in the first months of life, the CD38 marker is found on 195% of CD8 + T cells and is a marker of both lymphocyte immaturity and activation [36,37]. In the WITS, the association of early low percentage of CD8 + CD38 + cells with immunological progression was weaker than that of CD8 + HLA-DR + cells [23].…”

Section: Discussionmentioning

confidence: 97%