Immunoprotection by monoclonal antibodies to the porins and lipopolysaccharide ofSalmonella typhimurium

Cross-protection of Salmonella abortusovis, S. choleraesuis, S. dublin and S. gallinarum in mice induced by S. abortusovis and S. gallinarum: bacteriology and humoral immune response

“…In a murine model, anti-lipid A monoclonal [7] or anti-idiotype antibodies [13] protected mice against a lethal challenge with rough LPS. However, it was demonstrated that by using direct passive transfer of anti-LPS antibodies [20,21,28,29,37], LPS alone cannot fully account for the specificity of protection by antibodies. The specificity of cross-protection was previously studied [19], using Typhimurium, Enteritidis and Dublin for vaccination and challenge, with variants that differed in the main LPS antigen (0-4 or 0-9).…”

The C-Terminal Domain of Salmonella enterica Serovar Typhimurium OmpA Is an Immunodominant Antigen in Mice but Appears To Be Only Partially Exposed on the Bacterial Cell Surface

“…Serovar Typhimurium strains WB600 (wild-type) and SH5014 (rfa mutant) and E. coli strain HN705 (⌬ompC ompF::Tn5) have been previously described (37,40,42,45); serovar Typhimurium strains SA1627 (rfb [26]) and SL1917 (galE496 ompA202 [44]) were provided by Ken Sanderson and Bruce Stocker, respectively. Clinical isolates of enteric and nonenteric bacteria, as well as the culture media and growth conditions for enteric and nonenteric bacteria, were previously described (41).…”

“…These immune responses primarily occur against the lipopolysaccharide (LPS) and major outer membrane (OM) proteins, including porins and OmpA (1,7,21,28,40,42). However, the number and variety of antibodies with distinct specificities and the identity of epitopes that they recognize are not well understood.…”

“…Previous studies (reviewed in references 40 and 42) established the importance of O-antigenspecific antibodies in immunity to murine salmonellosis. The precise role of porins, however, in humoral immunity is controversial (reviewed in reference 40).…”

Immunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis

“…Most the antigenic determinants are believed to be located in the outer loops with unordered structure. In contrast, epitopes located in the transmembrane protein sites are conservative and have a high degree of homology in porins of Enterobacteriaceae microorganisms (Singh et al, 1996).…”