Immunoreactive proteins of <i>Saccharopolyspora rectivirgula</i> for farmer's lung serodiagnosis

Barrera, Coralie; Millon, Laurence; Rognon, Bénédicte; Quadroni, Manfredo; Roussel, Sandrine; Dalphin, Jean‐Charles; Court-Fortuné, Isabelle; Caillaud, D.; Jouneau, S.; Fellrath, Jean-Marc; Zaugg, Christophe; Reboux, Gabriel; Monod, Michel

doi:10.1002/prca.201400024

Cited by 13 publications

(17 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Patients ( n = 41) diagnosed with FLD pulmonary syndrome on the basis of consensus criteria [ 17 , 18 ] were consecutively recruited from the pneumology departments of five university hospitals in France and Switzerland from 2006 to 2012. Patient characteristics were described in detail in a previous study [ 13 ]. HEC ( n = 43) were recruited from the same areas.…”

Section: Methodsmentioning

confidence: 99%

“…The immunoproteomic approach previously described for identification of immunoreactive proteins from S . rectivirgula [ 13 ] was used in this study with only a few changes. Protein extracts from L .…”

Section: Methodsmentioning

confidence: 99%

“…A strategy consisting in using sera from patients with FLD to identify immunogenic proteins from environmental microorganisms was applied and allowed the selection of immunoreactive proteins from S . rectivirgula [ 13 ] and Aspergillus species [ 14 ]. Recombinant antigens from these two species were produced and proved to be effective in the serodiagnosis of FLD by enzyme-linked immunosorbent assay (ELISA).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Antigenic Proteins from Lichtheimia corymbifera for Farmer’s Lung Disease Diagnosis

Rognon

Barrera

Monod³

et al. 2016

PLoS ONE

Self Cite

View full text Add to dashboard Cite

The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer’s lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Antigenic Proteins from Lichtheimia corymbifera for Farmer’s Lung Disease Diagnosis

Rognon

Barrera

Monod³

et al. 2016

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…The conditions for growth and DNA extraction of S. rectivirgula were described previously ( 6 ). The whole genome was sequenced with the 454 FLX technology (454 Roche GS FLX; Roche, Mannheim, Germany).…”

Section: Genome Announcementmentioning

confidence: 99%

“…These sequence data will facilitate the production of recombinant immunogenic proteins that could be further used as antigens for improved and standardized assay for FLD diagnosis ( 6 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Principal Etiological Agent of Farmer's Lung Disease, Saccharopolyspora rectivirgula

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

Bacteria in Indoor and Occupational Settings

Barnes

2017

Exposure to Microbiological Agents in Indoor and Occupational Environments

View full text Add to dashboard Cite

Immunoreactive proteins of Saccharopolyspora rectivirgula for farmer's lung serodiagnosis

Abstract: The recombinant antigens produced in this study constitute a major step toward the improvement of diagnostic performance and the standardization of FLD serodiagnosis.

Cited by 13 publications

References 32 publications

Identification of Antigenic Proteins from Lichtheimia corymbifera for Farmer’s Lung Disease Diagnosis

Identification of Antigenic Proteins from Lichtheimia corymbifera for Farmer’s Lung Disease Diagnosis

Draft Genome Sequence of the Principal Etiological Agent of Farmer's Lung Disease, Saccharopolyspora rectivirgula

Bacteria in Indoor and Occupational Settings

Contact Info

Product

Resources

About