Immunoreactivity of Synthetic Peptides Derived from Proteins of
            <i>Cryptococcus Gattii</i>

Brandão, Rafael Melo Santos de Serpa; Martins, Liline Maria Soares; Andrade, Hélida Monteiro de; Faria, Angélica Rosa; Leal, Maria José Soares; Silva, Adalberto Socorro da; Wanke, Bodo; Lazéra, Márcia dos Santos; Vainstein, Marilene Henning; Mendes, Rinaldo Pôncio; Moris, Daniela Vanessa; Cavalcante, Ricardo de Souza; Monte, Semíramis Jamil Hadad do

doi:10.2217/fmb.14.49

Cited by 7 publications

(12 citation statements)

References 35 publications

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“…The proteomic methodology has been used for the identification of many agents of infectious diseases, such as bacteria [17,18], protozoa [19,20] and fungi [21][22][23][24]. Some proteomic studies have been performed to identify Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have used two-dimensional electrophoresis and mass spectrometry to isolate and identify proteins to be useful in the diagnosis and monitoring of treatment in Candida albincans [21,22], Cryptococcus gatti [24] and Aspergillus fumigatus [23]. Classification of abundant proteins present in the human blood serum in infections of P. brasiliensis and P. lutzii before treatment according to their participation in a A) biological function, B) molecular function and C) cell components.…”

Section: Discussionmentioning

confidence: 99%

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Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Sylvestre

Cavalcante

Silva

et al. 2018

Preprint

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The sensitivity of the double agar gel immunodiffusion test is about 90% in patients with untreated paracoccidioidomycosis (PCM), but it is much lower in cases of relapse. In addition, serum from patients with PCM caused by Paracoccidioides lutzii, frequent in the Midwest region of Brazil, do not react with the classical antigen obtained from Pb B-339. These findings showed the need for alternative diagnostic methods, such as biological markers through proteomics. The aim of this study was to identify biomarkers for the safe identification of PCM relapse and specific proteins that could distinguish infections caused by Paracoccidioides brasiliensis from those produced by Paracoccidioides lutzii. Proteomic analysis was performed in serum from 9 patients with PCM caused by P. brasiliensis, with and without relapse, from 4 patients with PCM produced by P. lutzii, and from 3 healthy controls. The comparative evaluation of the 29 identified plasma proteins suggested that the presence of the immunoglobulin (Ig) alpha-2 chain C region and the absence of Ig heavy chain V-III TIL indicate infection by P. lutzii. In addition, the absence of complement factor B protein might be a predictor of relapse. The evaluation of these proteins in a higher number of patients should be carried out in order to validate these findings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Sylvestre

Cavalcante

Silva

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…The proteomic methodology has been used for the identification of many agents of infectious diseases, such as bacteria [24,25], protozoa [26,27] and fungi [28][29][30][31]. Some proteomic studies have been performed to identify Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

“…Brandão et al [31] determined by immunoproteomics approach the immunoreactivity of synthetic peptides selected from proteins derived from C. gattii. Six out of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISA, were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity.…”

Section: Proteinmentioning

confidence: 99%

Ceruloplasmin, transferrin and apolipoprotein A-IIplay important hole in treatment’s follow-up of paracoccidioidomycosis patients.

Mendes¹

2018

Preprint

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Paracoccidioidomycosis (PCM) is a systemic disease caused by thermodymorphic fungi of the Paracoccidioides brasiliensis complex, (Paracoccidioides spp.). Patients with PCM reveal specific cellular immune impairment. Despite the effective treatment, quiescent fungi can lead to relapse, usually late, the serological diagnosis of which has been deficient. The present study was carried out with the objective of investigating a biomarker for the identification of PCM relapse and another molecule behaving as an immunological recovery biomarker; therefore, it may be used as a cure criterion. In the evolutionary analysis of the proteins identified in PCM patients, comparing those that presented with those that did not reveal relapse, 29 proteins were identified. The interactions observed between the proteins, using transferrin and haptoglobin, as the main binding protein, were strong with all the others. Patient follow-up suggests that cerulosplamin may be a marker of relapse and that transferrin and apolipoprotein A-II may contribute to the evaluation of the treatment efficacy and avoiding a premature decision.

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“…In the search for a faster and more specific test, Brandão et al [105] tested various synthetic peptides derived from immunoreactive proteins of Cryptococcusspp. Of these, six showed good results, which became promising candidate antigens for future diagnostic tests.…”

Section: Synthetic Peptidesmentioning

confidence: 99%

Synthetic Peptides as an Alternative Tool for the Diagnosis of Cryptococcosis

Brandão¹,

Martins²,

doMonte³

2016

Fungal Pathogenicity

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Cryptococcosis is an important systemic mycosis that threatens the lives of humans and animals. The disease is caused by two species of the genus Cryptococcus Cryptococcus neoformans and Cryptococcus gattii. The diagnosis of cryptococcosis is made through microscopy, fungal culture followed by biochemical tests, and detection of the cryptococcal capsular antigen CrAg . Despite the existence of an established diagnostic protocol, the search for new diagnostic tests is necessary due to the high incidence of the disease, with estimates of approximately million cases of cryptococcal meningitis per year and more than , deaths in patients infected with human immunodeficiency virus HIV , the potential for C. gattii to cause the disease in immunocompetent individuals, and the disease's rapid worldwide dissemination. With the development of biotechnology, synthetic peptides have opened up new possibilities as a source of pure epitopes and molecules for the diagnosis of various diseases, based on the detection of circulating antibodies. Synthetic peptides can also be used for the development of vaccines. Studies on Leishmaniasis, Chagas disease, paracoccidioidomycosis, tuberculosis, and, more recently, on cryptococcosis, among others, have shown that this approach shows potential for the early diagnosis of the disease, thus reducing the morbi-lethality of individuals affected by this infection and ultimately changing their prognosis.Keywords: Cryptococcosis, diagnosis, antigens, synthetic peptides, B cell, epitopes . IntroductionCryptococcosis is an important systemic mycosis that threatens the lives of humans and animals. It manifests primarily through respiratory system diseases and meningoencephalitis. Cryptococcosis is among the emergent fungal infections with significant morbi-lethality, and it is the fourth most frequent cause of opportunistic infection in human immunodeficiency © 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.virus HIV -positive patients. The disease is caused by two species of the genus Cryptococcus Cryptococcus neoformans and Cryptococcus gattii [ , ].C. neoformans has a worldwide distribution and is responsible for the high morbi-lethality in immunocompromised individuals with AIDS. In contrast, infections with C. gattii are prevalent in tropical and subtropical climate regions, and C. gattii primarily attacks immunocompetent hosts. However, C. gattii has also emerged in countries with temperate climates, e.g., Canada Vancouver and the U.S. Northwest, which demonstrates that the fungus may adapt to new environments and cause surges of infection in animals and humans [ -].Annually, AIDS-related cryptococcal meningitis is responsible for approximately % of the mortality in these individuals [ ]. Sub-Saharan Africa has the largest rate of coinfecti...

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Immunoreactivity of Synthetic Peptides Derived from Proteins of Cryptococcus Gattii

Abstract: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.

Cited by 7 publications

References 35 publications

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.

Ceruloplasmin, transferrin and apolipoprotein A-IIplay important hole in treatment’s follow-up of paracoccidioidomycosis patients.

Synthetic Peptides as an Alternative Tool for the Diagnosis of Cryptococcosis

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