Immunoscopy—a technique combining endoscopy and immunofluorescence for diagnosis of colorectal carcinoma

Keller, R; Winde, Günther; Eisenhawer, Christian; Herwig, Ralf; Terpe, Hans-Joachim; Domschke, Wolfram; Foerster, E. C.

doi:10.1016/s0016-5107(98)70349-9

Cited by 30 publications

(27 citation statements)

References 18 publications

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“…Several attempts using labeled CEAantibodies have also been reported (2). Fluorescence that is specific to gastrointestinal lesions has been observed in studies using isolated specimens (4,5). However, the use of fluorescence of ultraviolet-range wavelength result in interference from strong background noise (7) and possible damage the living tissue (6).…”

Section: Discussionmentioning

confidence: 99%

“…In the field of gastroenterology (4,5), attempts have been made to check for lesions using carcinoembryonic antigen (CEA) antibody labeled with fluorescent material. However, since the wavelength of fluorescence used in those studies was in the ultraviolet range, its use in vivo is limited due to safety concerns (6) and due to interference from some other sources of fluorescence (7).…”

Section: Introductionmentioning

confidence: 99%

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Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays. A Novel Diagnostic Method for Micro Cancers in the Digestive Tract.

et al. 1999

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Object An indocyanine green derivative (ICG-sulfo-OSu) was used as the labeling substance for monoclonal antibody, and a fluorescence imaging system appropriate for ICG-sulfo-OSu excitable by infrared rays (IR) was developed. The goal of this study was to demonstrate antibody labeling at the tissue level using this new imaging system. Materials and Methods ICG-sulfo-OSu labeled mouse anti-human carcinoembryonic antigen (CEA) monoclonal antibody, a newly developed imaging system, and an infrared ray microscope were employed in this experiment. Paraffin sections of humancolon cancer previously proven to have cross-reactivity to anti-CEA antibody were examined. Results Positive staining was seen as a brownish discoloration of oxidized 3,3'-diaminobenzidine tetrahydrochloride (DAB) in sections that reacted with ICG-sulfoOSu-labeled anti-CEA antibody, and the fluorescence was well-matched with the oxidized DABpositive sites. Conclusion Specific antibodies labeled with ICG-sulfo-OSu have significant affinity to cancer cells and seem to reflect sufficient amounts of fluorescence by IR to be useful in a system for the endoscopic detection of micro cancers using the immunohistochemical staining method. (Internal Medicine 38: 537-542, 1999)

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays. A Novel Diagnostic Method for Micro Cancers in the Digestive Tract.

et al. 1999

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“…Similarly, in the field of GI endoscopy, the authors propose rapid development of 'endoscopic molecular imaging', which is considered to be divided into three categories: (a) visualization of cell morphology on the micro to nano level; (b) reflection of spectroscopic characteristics; and (c) visualization of molecular characteristics. The future of endoscopic diagnosis is likely to be affected by a combination of biomarkers and technology (Takayama et al, 1998), and 'endoscopic molecular imaging' would be defined as (c), which has been described as 'immunoscopy' (Keller et al, 1998), 'bioendoscopy' (Pasricha & Motamedi, 2002), and 'optical biopsy' (Fujimoto et al, 1995). These innovations will allow us not only to locate a tumor but also useful to 1) differentiate malignant and benign polyps and ulcers, 2) minimize number of biopsies and frequency of surveillance, 3) accurate preoperative identification of tumor margin, 4) evaluate effectiveness of pharmacological therapy, 5) detect local dysplasia in Barrett's mucosa or ulcerative colitis.…”

Section: Introductionmentioning

confidence: 99%

Endoscopic Molecular Imaging in Gastrointestinal Oncology

Muguruma¹,

Takayama²

2011

New Techniques in Gastrointestinal Endoscopy

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“…Further, fluorescence imaging can permit monitoring of the progress of accumulation and destruction of the photosensitiser both before and during PDT treatments, which can aid in the clinical assessment of the appropriateness of drug and light dosing at the time of treatment [9]. ALA-based PD and PDT is used in a variety of applications [1], including endoscopic detection of lesions [12][13][14] and on-line determination of treatment end-points by kinetic fluorescence measurements [15].…”

Section: Introductionmentioning

confidence: 99%

“…Various devices suitable for this application have already been developed [9,12,14]. We have assembled an a#ordable, portable fluorescence imaging prototype for skin lesion detection.…”

Section: Introductionmentioning

confidence: 99%

An Affordable, Portable Fluorescence Imaging Device for Skin Lesion Detection Using a Dual Wavelength Approach for Image Contrast Enhancement and Aminolaevulinic Acid-induced Protoporphyrin IX. Part II. In Vivo Testing

et al. 2001

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A fluorescence imaging prototype for skin lesion detection and diagnosis using aminolaevulinic acid (ALA) induced protoporphyrin IX (PpIX) was tested in vivo and in the clinic. The prototype was designed as an affordable, portable device to allow contrast enhanced imaging of skin lesions using either the dual excitation wavelength method or the dual emission wavelength method or both. In this study the prototype was tested first on an animal model. Topical application of ALA on defined spots on mouse skin gave PpIX fluorescence after about 3 hours of application. After successful in vivo testing the instrument was tested on basal cell carcinoma patients before ALA-PpIX photodynamic therapy. The patients were topically applied with ALA. After three hours the device was tested (immediately before treatment). The prototype showed good results in terms of contrast enhancement (elimination of unwanted background signals, e.g. autofluorescence) using either contrast enhancement method, both methods achieving similar results. The results achieved in this study, combined with the affordable design of the device, seem to allow cost-effective, contrast-enhanced imaging of skin lesions before or during photodynamic therapy using ALA induced PpIX.

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Immunoscopy—a technique combining endoscopy and immunofluorescence for diagnosis of colorectal carcinoma

Cited by 30 publications

References 18 publications

Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays. A Novel Diagnostic Method for Micro Cancers in the Digestive Tract.

Labeled Carcinoembryonic Antigen Antibodies Excitable by Infrared Rays. A Novel Diagnostic Method for Micro Cancers in the Digestive Tract.

Endoscopic Molecular Imaging in Gastrointestinal Oncology

An Affordable, Portable Fluorescence Imaging Device for Skin Lesion Detection Using a Dual Wavelength Approach for Image Contrast Enhancement and Aminolaevulinic Acid-induced Protoporphyrin IX. Part II. In Vivo Testing

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