Immunostimulatory Activity of Aqueous Extract of Polyherbal Formulation on Th1/Th2 Cytokines Secretion and Cell Mediated Immune Response in Rats

Hamid, KM; Sy, Isah; Mu, Kalgo; Isiyaku, Abdullahi; Ab, Usman; Yeldu, MH; Yusuf, Abdurrahman Pharmacy; Nk, Abubakar; Yb, Garba; Hy, Muhammad

doi:10.36348/sjmps.2021.v07i01.013

Cited by 3 publications

(1 citation statement)

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“…(Senegalia senegal (L) Britton) bark, black cutch (Acacia polyacantha willd: Senegalia polyacantha (Willd) Seigler & Ebinger)bark, and garlic (Allium sativum (L): Foeniculum vulgare Kazakova) yellow bulb. Previous studies on the PHF have shown improvements in neutrophil adhesion and T-dependent antibody response (Isah et al, 2018), and exerted immunostimulatory activity on the production of Th1/Th2 cytokines and the rat cellmediated immune response (Hamid et al, 2021a). The PHF also confers immunomodulatory activity on mice's macrophage function and humoral immunity to sheep red blood cells (Hamid et al, 2021b).…”

Section: Introductionmentioning

confidence: 95%

Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

Usman

Gana

Hamid

et al. 2022

USci

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Medicinal plants have been widely used for medicinal purposes in the treatment of various ailments including human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). This study evaluated the effect of aqueous extracts of polyherbal formulation (PHF) on peripheral blood mononuclear cells (PBMCs) of an HIV+ patient. In this study, 4 ml of blood was collected from HIV+ patient who has not commenced antiretroviral treatment. PBMCs were isolated using the Ficoll-Paque method and counted using the trypan blue assay. The isolated PBMCs were treated with different concentrations of the PHF (25 50, 100, and 200 μg/ml). Cytotoxicity and proliferation were determined using the WST-8 assay. The percentage viability in this study was 96.74%, whereas the non-viable cell percentage was 3.26%. The lowest percentage of cytotoxicity for the PHF was 0.05% at 25 µg/ml of the extract, while the highest was 5.35% at 200 µg/ml. The mean absorbance scores of the formulation's various concentrations and the control group did not significantly differ from one another (F=0.622, df = 5, p=0.69). There was significant difference (t= -4.887, df =6, 95% CI= -0.1566 to -0.0521, p = 0.003) in mean absorbance scores between control and treatment. The polyherbal formulation exerts the lowest cytotoxicity of 0.05% and significant proliferation on the PBMCs at 25 µg/ml. The PHF has potential immunoproliferative activity with a good safety margin. An in-depth study of the bioactivity of the PHF on individual immune cells is recommended.

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Section: Introductionmentioning

confidence: 95%

Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

Usman

Gana

Hamid

et al. 2022

USci

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Acute oral toxicity study of novel polyherbal formulations by using wistar rats and Swiss albino mice as per OECD 425 TG

Jatoth,

Dhanabal,

Senthil

et al. 2025

Phytomedicine Plus

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Immunomodulatory Activity of Aqueous Extract of Polyherbal Formulation on Macrophage Function and Humoral Immune Response against SRBCs in Mice

Hamid¹,

Shehu²,

Kalgo³

et al. 2021

NJPAS

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Several herbal formulation were not properly documented due to poor scientific data as well as poor standard regulation in preparation and marketing. The study evaluate the effect of aqueous extract of Polyherbal formulation on Macrophages’ phagocytic function and Humoral immune response in Mice A total of Sixteen 16 Mice was used. Group I received normal saline, Group II-IV received 500 mg, 1000 mg, and 1500 mg of Polyherbal formulation respectively for 21 days each. The animals were sensitized and challenged with Sheep red blood cells at day 14th and 19th of the treatment respectively. On day 21st all the animals were injected with 0.1 ml Indian ink for carbon clearance assay and blood sample was collected at 1 minute and 15 minutes of the injection. The phagocytic function of Macrophages and humoral immune response were determined spectrophotometrically and Hemagglutination assay respectively. The results show that the highest carbonic particle clearances index (K) median score (Median=0.0228), Macrophage phagocytose index (α) median score (Median=3.249), organ weight index (g/100g) mean score (M=0.06633) and Hemagglutination antibody titre median score (Median=32.00) was recorded by Group IV when compared with other groups. The carbonic particle clearance (K) (p=0.02), phagocytic index of Macrophage (α) (p=0.03), and organ weight index (g/100g) (p<0.0001) significantly increase with increase in the Polyherbal formulation concentration. Hemagglutination antibody titre against Sheep red blood cells (p=0.02) also significantly increased. The extract has potential immunostimulatory activity on both Macrophages’ phagocytic function and humoral immune response in mice thus could be useful in improving immune responses.

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Immunostimulatory Activity of Aqueous Extract of Polyherbal Formulation on Th1/Th2 Cytokines Secretion and Cell Mediated Immune Response in Rats

Cited by 3 publications

References 0 publications

Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

Immunotoxicity and proliferation of human peripheral blood mononuclear cells of HIV-positive patient after treatment with aqueous extract of polyherbal formulation

Acute oral toxicity study of novel polyherbal formulations by using wistar rats and Swiss albino mice as per OECD 425 TG

Immunomodulatory Activity of Aqueous Extract of Polyherbal Formulation on Macrophage Function and Humoral Immune Response against SRBCs in Mice

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