Immunostimulatory<i>in Vitro</i>and<i>in Vivo</i>Effects of a Water-Soluble Extract from Kale

Nishi, Kosuke; Kondo, Ai; Okamoto, Takeaki; Nakano, Hiroyuki; Daifuku, Miho; Nishimoto, Sogo; Ochi, Kenji; Takaoka, Terumi; Sugahara, Takuya

doi:10.1271/bbb.100490

Cited by 18 publications

(19 citation statements)

References 24 publications

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“…On the other hand, T cells also play an important role in the cellmediated immunity. We have focused on the foodstuffs that activate the immune system for development of functional foods (Sugahara et al 2005;Nishi et al 2011Nishi et al , 2012Morishige et al 2011;Daifuku et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

et al. 2013

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Bengkoang (Pachyrhizus erosus (L.) Urban) is one of the most popular edible root vegetables in Indonesia. Bengkoang contains fairly large amounts of carbohydrates and crude fiber. The purpose of this research is to evaluate the immunomodulatory effect of the bengkoang fiber extract (BFE) in vitro and in vivo. BFE was prepared by heating the powder of bengkoang fiber suspended in distilled water at 121°C for 20 min. BFE facilitated IgM production by the human hybridoma cell line HB4C5 cells. In addition, production of IgM, IgG, and IgA by mouse primary splenocytes was facilitated by BFE in a dose-dependent manner. BFE also significantly facilitated production of both interleukin-5 and interleukin-10 by splenocytes. Immunoglobulin production by lymphocytes from the spleen, Peyer's patch, and mesenteric lymph node were significantly activated by oral administration of BFE to mice for 14 days. The serum immunoglobulin levels of IgG, IgM, and IgA were also significantly enhanced. Furthermore, cytokine production by lymphocytes from the spleen, Peyer's patch, and mesenteric lymph node were also facilitated by oral administration of BFE. These results suggest that BFE has positive effects on the immune system in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

et al. 2013

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“…Real-time RT-PCR was performed as described elsewhere. 12) Briefly, HB4C5 cells were cultured at about 5:0 Â 10 4 cells/mL in ITES-ERDF medium with and without heated yellowtail heart extract for 6 h at 37 C. Then the cells were harvested, and total RNA was extracted using a Sepasol RNA I Super G (Nacalai Tesque, Kyoto, Japan) following the manufacturer's instructions. First-strand cDNAs were synthesized from total RNAs using oligo-dT primer and MMLV reverse transcriptase (Promega, Madison, WI).…”

Section: Methodsmentioning

confidence: 99%

Immunostimulation Effects of Yellowtail Heart Extractsin Vitroandin Vivo

Daifuku

Yaguchi

Nishi

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…First-strand cDNA synthesis and real-time RT-PCR using Fast SYBR green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) were performed as described elsewhere (Nishi et al, 2011). Expression of β-actin gene was used as an endogenous control and the data were shown as the fold difference normalized to β-actin.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Phenylhydroquinone induces loss of thymocytes through cell cycle arrest and apoptosis elevation in p53-dependent pathway

et al. 2013

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-ortho-Phenylphenol has been employed in post-harvest treatment of citrus fruits. Although o-phenylphenol has been reported to cause carcinomas in the urinary tract in rats, toxicity to the immune organs is still unknown. Herein, we report that administration of o-phenylphenol induces thymic atrophy and loss of thymocytes in female BALB/c mice. The influence seems to result from inhibition of the thymocyte development, because increased and decreased populations of the CD4 -CD8 -double-negative and CD4 + CD8 + double-positive thymocytes were observed in the o-phenylphenol-administered mice, respectively. ortho-Phenylphenol is metabolized to phenylhydroquinone by cytochrome P450 monooxygenases. Phenylhydroquinone made cell cycle of thymocytes to be arrested through reduced expression of the genes associated with G 2 /M phase and through phosphorylation of p53 at Ser15. Phosphorylation of p53 at Ser15 was upregulated by activation of not only ATR but also Erk1/2 and p38, leading to increase of apoptosis. Gene expression of cytochrome P450 1A1 (CYP1A1) was promoted in thymocytes from the o-phenylphenol-administered mice. Overall, our results suggest that o-phenylphenol induces CYP1A1 expression and is metabolized into phenylhydroquinone by the expressed CYP1A1 in thymocytes. The produced phenylhydroquinone in turn induces inhibition of thymocyte development through cell cycle arrest and apoptosis in the p53-dependent pathway.

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Immunostimulatoryin Vitroandin VivoEffects of a Water-Soluble Extract from Kale

Cited by 18 publications

References 24 publications

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

Immunostimulation Effects of Yellowtail Heart Extractsin Vitroandin Vivo

Phenylhydroquinone induces loss of thymocytes through cell cycle arrest and apoptosis elevation in p53-dependent pathway

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