Immunosuppressive and anti-inflammatory properties of a major protein secreted from the epithelium of the rat seminal vesicles

Metafora, Salvatore; Peluso, Gianfranco; Persico, Paola; Ravagnan, G; Esposito, Carla; Porta, Raffaele

doi:10.1016/0006-2952(89)90158-5

Cited by 56 publications

(77 citation statements)

References 25 publications

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“…These data confirm the immunosuppressive activity of SV-IV previously observed in other experimental settings (Paonessa et al, 1984;Metafora et al, 1989;Peluso et al, 1994;Romano-Carratelli et al, 1995;RomanoCarratelli et al, 2002).…”

Section: Discussionsupporting

confidence: 91%

“…2).These data are consistent with similar binding observations already published (Metafora et al, 1989;Morelli et al, 2007). (Stiuso et al, 1999;Romano-Carratelli et al, 2002).…”

Section: Sv-iv Fitc and [ 125 I]sv-iv Binding To The Surface Of Humansupporting

confidence: 92%

“…Similar results were obtained with resting or antigen-stimulated CTLs. In addition, the possibility that the immunosuppression was due to aspecific cytotoxic properties can be ruled out by the following observations: 1) immunosuppression was detectable on activated MNC (see Results) only at low concentrations (5 to 30 M), rich in biologically active SV-IV monomers; at similar low concentrations SV-IV did not inhibit but, in contrast, stimulated quiescent lymphocytes to synthesize and release specific cytokines (Tufano et al, 1996); 2) at concentrations higher than 30 M, rich in biologically inactive SV-IV trimers, no cytotoxic effects were observed (Stiuso et al, 1999); 3) the inhibitory effects of SV-IV on proliferation were found only for immunocompetent cells (Metafora et al, 1989); 4) in addition, SV-IV has been recently demonstrated to possess intrinsic antiapoptotic activity (Morelli et al, 2007).…”

Section: Discussionmentioning

confidence: 96%

“…SV-IV possesses a nonspecies-specific ability to modulate inflammation and humoral and cellmediated immune response (Metafora et al, 1989;Peluso et al, 1994;Romano-Carratelli et al, 1995;Tufano et al, 1996;Santagada et al, 2002), to stimulate horseradish and Se-dependent peroxidases (Metafora et al, 2001 and unpublished data), to protect its target cells against oxidative stress-associated apoptosis and, in particular, to defend nonself epididymal spermatozoa from immunological or reactive oxygen species (ROS) attacks in the female genital tract (Paonessa et al, 1984;Morelli et al, 2007). The anti-inflammatory properties are related to phospholipase A 2 (PLA 2 ) inhibition (Metafora et al, 1989), whereas the pro-inflammatory features are based on SV-IV ability to stimulate mast-cell and basophil degranulation with concurrent release of histamine and other mediators (Metafora et al, manuscript in preparation).…”

Section: Introductionmentioning

confidence: 99%

“…The anti-inflammatory properties are related to phospholipase A 2 (PLA 2 ) inhibition (Metafora et al, 1989), whereas the pro-inflammatory features are based on SV-IV ability to stimulate mast-cell and basophil degranulation with concurrent release of histamine and other mediators (Metafora et al, manuscript in preparation). The modulation of immune response is caused by interference of the protein with macrophage-T cell cooperation (Metafora et al, 1989;Peluso et al, 1994;Romano-Carratelli et al, 1995;Tufano et al, 1996;Santagada et al, 2002). Natural killer cells (NKs) and antigen-specific cytotoxic T lymphocytes (CTLs) are involved in host resistance to infections, immune surveillance against tumors, and rejection of transplanted tissues.…”

Section: Introductionmentioning

confidence: 99%

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Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

Fuggetta

Lanzilli

Cottarelli

et al. 2008

Journal of Reproductive Immunology

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Section: Discussionsupporting

confidence: 91%

“…2).These data are consistent with similar binding observations already published (Metafora et al, 1989;Morelli et al, 2007). (Stiuso et al, 1999;Romano-Carratelli et al, 2002).…”

Section: Sv-iv Fitc and [ 125 I]sv-iv Binding To The Surface Of Humansupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

Fuggetta

Lanzilli

Cottarelli

et al. 2008

Journal of Reproductive Immunology

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SV-IV, a major protein secreted from rat seminal vesicle epithelium, promotes lymphocyte cytotoxic activity against the lymphoblastoid Raji cell line in human peripheral blood mononuclear cells

et al. 1997

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The treatment of human peripheral blood mononuclear cells (PBMC) with micromolar concentrations of SV-IV, a major protein secreted from the rat seminal vesicle epithelium, promotes in this cell population a marked cytotoxic activity against the Raji lymphoblastoid cell line. This activity is apparently due to cell-to-cell contact interactions. The expression of HLA DR on Raji cells has a modulatory effect on the SV-IV-induced cytotoxic activity. The experimental evidence strongly suggests that the cytotoxic effector cells are functionally activated NK cells.

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Structural heterogeneity, post-translational modifications, and biological activities of SV-IV, a major protein secreted from the rat seminal vesicle epithelium

Ferranti¹,

Mamone²,

Malorni³

et al. 1997

Rapid Commun. Mass Spectrom.

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The primary structure of purified SV-IV, a major secretory protein synthesized by the rat seminal vesicle (SV) epithelium, was analysed by electrospray mass spectrometry (ES-MS). The protein was found to be highly heterogeneous. The various components were separated and identified by reversed phase high-performance liquid chromatography (HPLC) on line with ES-MS. Structural characterization of the SV-IV cyanogen bromide digests revealed the occurrence of a Val/Met substitution in about 50% of the purified protein molecules. We suggest that this mutation is the expression of a genetic polymorphism. Other minor components, corresponding to structural changes (fragmentation, deletion, and phosphorylation) of SV-IV and probably due to post-translational modifications of the native protein, were also detected. In particular, by using protein tyrosine phosphatase hydrolysis combined with ES-MS, we demonstrated that, in the phosphorylated species of SV-IV, a single phosphate group was covalently bound to the Tyr-36 residue. The significance of these findings in relation to the regulation of important biological processes, such as immune response, blood coagulation, inflammatory reaction, and mammalian reproduction, are discussed.

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Immunosuppressive and anti-inflammatory properties of a major protein secreted from the epithelium of the rat seminal vesicles

Cited by 56 publications

References 25 publications

Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity

SV-IV, a major protein secreted from rat seminal vesicle epithelium, promotes lymphocyte cytotoxic activity against the lymphoblastoid Raji cell line in human peripheral blood mononuclear cells

Structural heterogeneity, post-translational modifications, and biological activities of SV-IV, a major protein secreted from the rat seminal vesicle epithelium

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