Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells in Cultures with Different O2 Content in the Medium

Горностаева, А. Н.; Andreeva, E. R.; Андрианова, И. В.; Буравкова, Л. Б.

doi:10.1007/s10517-011-1372-2

Cited by 6 publications

(9 citation statements)

References 18 publications

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“…For instance, MSCs did not affect lymphocyte proliferation after 24 h of co-culturing, and reduced this activity 48 and 72 h later (Glennie et al 2005). According to our data, effective inhibition of PHA-activated lymphocytes proliferation also occurs after 72 h of interaction with MSCs (Gornostaeva et al 2011(Gornostaeva et al , 2013. Inhibition of T-cell activation becomes more distinct with an extension of the co-culturing time (Puissant et al 2005;Yang et al 2009).…”

Section: Time Of Interactionsupporting

confidence: 52%

“…On the contrary, IFN-c synthesis increased in the co-culture of MSCs and aCD3/aCD28-stimulated lymphocytes (Kronsteiner et al 2011). According to our data, in the presence of MSCs, the IFN-c and IL-10 production by PHA-stimulated lymphocytes increased and TNF-a decreased (Gornostaeva et al 2011(Gornostaeva et al , 2013. In the co-culture with MSCs, the CD3/CD28-activated T-cells demonstrated a significant increase in apoptotic cells (Yang et al 2009;Park et al 2011); at the same time, the share of apoptotic cells did not change among ConA and PHA-stimulating lymphocytes (Zappia et al 2005;Gornostaeva et al 2011Gornostaeva et al , 2013.…”

Section: Activation Of Immune Cellsmentioning

confidence: 54%

“…Suppression of MLR-activated cells was distinct only when the MSCs/lymphocytes ratio was as high as 1:1, 1:2 and 1:4 (McIntosh et al 2006). Our findings show that MSCs are capable of effectively suppressing the proliferation of PHA-stimulated lymphocytes at the ratio of 1:10 (Gornostaeva et al 2011(Gornostaeva et al , 2013 (Table 1).…”

Section: Mscs/immune Cells Ratiomentioning

confidence: 64%

“…The same relationship was observed in the absence of a direct cell contact between MSCs and T-cells (Suva et al 2008). According to others, MSCs were able to suppress lymphocyte proliferation effectively when only PHA was used for activation (Puissant et al 2005;Kronsteiner et al 2011;Gornostaeva et al 2011Gornostaeva et al , 2013.…”

Section: Activation Of Immune Cellsmentioning

confidence: 94%

“…In our lab we performed a comparative analysis of the immunosuppressive effects of the adhered and suspended MSCs. It turned out that suspended cells have a stronger antiproliferative effect on the T-lymphocytes, but their ability to down-regulate lymphocyte activation does not differ from that of adhered MSCs (Gornostaeva et al 2011(Gornostaeva et al , 2013.…”

Section: Culture Conditions and Msc Immunosuppressionmentioning

confidence: 99%

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Factors governing the immunosuppressive effects of multipotent mesenchymal stromal cells in vitro

2015

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Multipotent mesenchymal stem/stromal cells (MSCs) are of great interest to researchers because of the unique properties, such as enhanced proliferation, paracrine activity and multilineage differentiation. Their non-immunogenicity, in combination with immunomodulatory properties, opens up the opportunity for the allogeneic application of MSCs. The MSC immunomodulatory capacity is currently being actively studied in vitro using various experimental designs. However, the results are not always univocal. It was found that the outcome of the stromal/immune cell interaction depends on experimental conditions. In this review we considered the impact of different factors, such as the ratio of stromal/immune cells, interaction time, the path of immune cell activation, etc. on the MSC immunomodulation. We also accentuated the importance of local milieu, in particular, oxygen tension, for the realization of MSC immunosuppressive activity.

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Section: Time Of Interactionsupporting

confidence: 52%

Section: Activation Of Immune Cellsmentioning

confidence: 54%

Section: Mscs/immune Cells Ratiomentioning

confidence: 64%

Section: Activation Of Immune Cellsmentioning

confidence: 94%

Section: Culture Conditions and Msc Immunosuppressionmentioning

confidence: 99%

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Factors governing the immunosuppressive effects of multipotent mesenchymal stromal cells in vitro

2015

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Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro

Mahmoud,

Abdel-Rasheed,

Galal

et al. 2023

Stem Cell Rev and Rep

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Human adipose tissue-derived stem/stromal cells (hASCs) are adult multipotent mesenchymal stem/stromal cells with immunomodulatory capacities. Here, we present up-to-date knowledge on the impact of different experimental and donor-related factors on hASC immunoregulatory functions in vitro. The experimental determinants include the immunological status of hASCs relative to target immune cells, contact vs. contactless interaction, and oxygen tension. Factors such as the ratio of hASCs to immune cells, the cellular context, the immune cell activation status, and coculture duration are also discussed. Conditioning of hASCs with different approaches before interaction with immune cells, hASC culture in xenogenic or xenofree culture medium, hASC culture in two-dimension vs. three-dimension with biomaterials, and the hASC passage number are among the experimental parameters that greatly may impact the hASC immunosuppressive potential in vitro, thus, they are also considered. Moreover, the influence of donor-related characteristics such as age, sex, and health status on hASC immunomodulation in vitro is reviewed. By analysis of the literature studies, most of the indicated determinants have been investigated in broad non-standardized ranges, so the results are not univocal. Clear conclusions cannot be drawn for the fine-tuned scenarios of many important factors to set a standard hASC immunopotency assay. Such variability needs to be carefully considered in further standardized research. Importantly, field experts’ opinions may help to make it clearer. Graphical Abstract Parameters that promote ASC immunosuppression on immune cells. Activation of immune cells induces their proliferation and differentiation and presence of ASCs modulates/suppresses such consequences. Augmented immunosuppressive effects of ASCs can be introduced in direct contact with the immune cells and via complementing the repeatedly reported experimental settings (texts in grey shapes). Abbreviations: ASCs: adipose tissue-derived stem/stromal cells, IFN-ɤ: Interferon gamma, MLR: Mixed lymphocyte reaction, TNF: Tumor necrosis factor.

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Interaction of multipotent mesenchymal stromal and immune cells: Bidirectional effects

et al. 2017

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Immunosuppressive Effects of Multipotent Mesenchymal Stromal Cells in Cultures with Different O2 Content in the Medium

Cited by 6 publications

References 18 publications

Factors governing the immunosuppressive effects of multipotent mesenchymal stromal cells in vitro

Factors governing the immunosuppressive effects of multipotent mesenchymal stromal cells in vitro

Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro

Interaction of multipotent mesenchymal stromal and immune cells: Bidirectional effects

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