Immunotherapy for the treatment of canine transmissible venereal tumor based in dendritic cells pulsed with tumoral exosomes

Ramos-Zayas, Yareellys; Franco‐Molina, Moises Armides; Hernádez-Granados, Alex Jesús; Zárate-Triviño, Diana; Coronado‐Cerda, Erika Evangelina; Mendoza‐Gamboa, Edgar; Zapata‐Benavides, Pablo; Ramírez-Romero, Rafael; Santana-Krymskaya, Silvia Elena; Taméz-Guerra, Reyes; Rodríguez‐Padilla, Cristina

doi:10.1080/08923973.2018.1533969

Cited by 20 publications

(9 citation statements)

References 12 publications

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“…Characterization and quantification of the exosomes from the primary culture of the canine transmissible venereal tumor were performed using scanning electron microscopy (SEM) as part of immunotherapy for treating the tumor [ 107 ]. EVs from human milk were also quantified using SEM and it was observed that the size of these nanovesicles was in the range between 50 and 350 nm [ 108 ].…”

Section: Methods Of Quantificationmentioning

confidence: 99%

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

et al. 2021

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Exosomes are the smallest extracellular vesicles present in most of the biological fluids. They are found to play an important role in cell signaling, immune response, tumor metastasis, etc. Studies have shown that these vesicles also have diagnostic and therapeutic roles for which their accurate detection and quantification is essential. Due to the complexity in size and structure of exosomes, even the gold standard methods face challenges. This comprehensive review discusses the various standard methods such as ultracentrifugation, ultrafiltration, size-exclusion chromatography, precipitation, immunoaffinity, and microfluidic technologies for the isolation of exosomes. The principle of isolation of each method is described, as well as their specific advantages and disadvantages. Quantification of exosomes by nanoparticle tracking analysis, flow cytometry, tunable resistive pulse sensing, electron microscopy, dynamic light scattering, and microfluidic devices are also described, along with the applications of exosomes in various biomedical domains.

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Section: Methods Of Quantificationmentioning

confidence: 99%

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

et al. 2021

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“…99 Tumor cell-derived exosomes are a good source of cellular components that stimulate the immune response, such as alarmins (mRNA, transmembrane proteins including CD9, CD63, and CD81, HSPs, major histocompatibility complex I molecules) and tumor-associated antigens. 100 The antigen-presenting and immune-stimulating properties of TEXs enable them to trigger antitumor responses and contribute to the recruitment and reconstruction of tumor microenvironmental components. 101 For example, TEXs derived from oral squamous cell carcinoma and histiocytic lymphoma could induce immunosuppression through the CD95 (Fas) receptor and FasL + exosomes signaling on activated CD8 + T cells.…”

Section: Exosome and Cancer-associated Fibroblastsmentioning

confidence: 99%

Exosomes: key players in cancer and potential therapeutic strategy

Dai

Zhong

et al. 2020

Sig Transduct Target Ther

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Exosomes are extracellular vesicles secreted by most eukaryotic cells and participate in intercellular communication. The components of exosomes, including proteins, DNA, mRNA, microRNA, long noncoding RNA, circular RNA, etc., which play a crucial role in regulating tumor growth, metastasis, and angiogenesis in the process of cancer development, and can be used as a prognostic marker and/or grading basis for tumor patients. Hereby, we mainly summarized as followed: the role of exosome contents in cancer, focusing on proteins and noncoding RNA; the interaction between exosomes and tumor microenvironment; the mechanisms that epithelial-mesenchymal transition, invasion and migration of tumor affected by exosomes; and tumor suppression strategies based on exosomes. Finally, the application potential of exosomes in clinical tumor diagnosis and therapy is prospected, which providing theoretical supports for using exosomes to serve precise tumor treatment in the clinic.

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“…Las células tumorales presentan un crecimiento muy rápido, lo que da lugar a una masa tumoral importante y a una gran destrucción de los tejidos locales (9). El TVT se transmite como aloinjerto, principalmente durante el coito (10,11), aunque también puede transmitirse por mordedura, olfateo y lamido de laceración; así, también puede encontrarse en la piel de la cara, las fosas nasales, la cavidad oral y el interior/alrededor de los ojos; ocasionalmente, se ha encontrado en el bazo, el hígado, el cerebro y los pulmones de perros infectados, lo que indica su capacidad de metástasis (9,12).…”

Section: Introductionunclassified

Prevalence and distribution of transmissible venereal tumor in dogs from a rural community of Mexico

et al. 2024

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Objetivo: Determinar la tasa de prevalencia y recurrencia de infección por TVT en perros con propietario y la distribución de la enfermedad en Cuajinicuilapa, Guerrero, México. Métodos: Estudio observacional, descriptivo y longitudinal. Se investigó la presencia de TVT en todos los perros de la comunidad con base en la historia clínica, examen físico y evaluación citológica, y el diagnóstico se confirmó por histopatología. Se retiraron quirúrgicamente muestras de tejido para análisis histopatológico y, cuando se requirió, para eliminar los tumores y tratar con vincristina; se identificaron posibles agrupaciones espaciales por GPS. Todos los casos se reevaluaron un año después. Resultados: Se encontró una prevalencia de 5.15% en 1047 perros (512 hembras y 535 machos; 25 hembras positivas y 29 machos positivos). La infección por TVT fue más frecuente en perros de 2-3 años. Se observó un patrón de agregación espacial en los casos locales. No hubo lesiones en los 29 animales tratados un año después. Conclusión: El TVT es una enfermedad común, y su distribución sugiere su agregación en ciertas áreas de la comunidad. Se requieren más estudios sobre la dinámica de la población de perros en ciudades pequeñas.

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Immunotherapy for the treatment of canine transmissible venereal tumor based in dendritic cells pulsed with tumoral exosomes

Cited by 20 publications

References 12 publications

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

Elucidating Methods for Isolation and Quantification of Exosomes: A Review

Exosomes: key players in cancer and potential therapeutic strategy

Prevalence and distribution of transmissible venereal tumor in dogs from a rural community of Mexico

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