Immunotherapy with regulatory T and B cells in periodontitis

Zou, Juan; Zeng, Zijun; Xie, Wen; Zeng, Zhimei

doi:10.1016/j.intimp.2022.108797

Cited by 13 publications

(8 citation statements)

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“…The marginal zone is dominated by B cells and is an important site for recognizing antigens and inducing immune responses. Immune cells are the basis of the immune functions of the spleen, which can exert specific immune functions in the body through T cell‐mediated cellular immunity and B‐cell‐mediated humoural immunity 27 . The main function of the thymus is to produce T lymphocytes and participate in cellular immunity 28 .…”

Section: Discussionmentioning

confidence: 99%

“…Immune cells are the basis of the immune functions of the spleen, which can exert specific immune functions in the body through T cellmediated cellular immunity and B-cell-mediated humoural immunity. 27 The main function of the thymus is to produce T lymphocytes and participate in cellular immunity. 28 The pathological results showed that in fibulin-2-knockout mice, both spleen function and thymus function were impaired, and the corresponding CD4+ and CD8+ T cells and CD19+ B cells, which perform immune functions, were reduced.…”

Section: Discussionmentioning

confidence: 99%

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Fibulin‐2: A potential regulator of immune dysfunction after bone trauma

Jiang

Wang

et al. 2023

Immunity Inflam & Disease

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Objectives To reveal the relationship between the fibulin‐2 protein and immune dysfunction after bone trauma. Methods Individuals who were admitted to the study were divided into a bone trauma group, a recovered from bone trauma group and a volunteer without bone trauma group based on the reason for admission. Fibulin‐2 levels in the three groups were compared. Fibulin‐2‐knockout (fibulin‐2−/−) mice and wild‐type (WT) mice were used to detect susceptibility to infection. Hematoxylin and eosin (HE) staining and immunohistochemical staining were employed to observe pathological changes in each organ from fibulin‐2−/− mice and WT mice. Results In total, 132 patients were enrolled in this study. The fibulin‐2 level in the bone trauma group was lower than that in the recovered bone trauma group (3.39 ± 1.41 vs. 4.30 ± 1.38 ng/mL, t = 2.948, p < .05) and also lower than that in the volunteers without bone trauma group (3.39 ± 1.41 vs. 4.73 ± 1.67 ng/mL, t = 4.135, p < .05). Fibulin‐2−/− mice are more prone to infection. Compared with those in WT mice, spleen function and thymus function in fibulin‐2−/− mice were impaired. Immunohistochemical staining revealed that compared with those in WT mice, significantly fewer CD4+ T cells, CD8+ T cells, and CD19+ B cells were noted in the spleen and thymus of fibulin‐2−/− mice. Conclusions The plasma fibulin‐2 level was lower in patients with bone trauma. Decreased fibulin‐2 is associated with immune dysfunction after bone trauma.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fibulin‐2: A potential regulator of immune dysfunction after bone trauma

Jiang

Wang

et al. 2023

Immunity Inflam & Disease

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“…In Dentistry, numerous studies showed the fundamental role of Treg cells in inhibiting pathological oral diseases such as periodontal disease (Cavalla et al., 2021; Han et al., 2018; Parachuru et al., 2018; Zou et al., 2022) and periapical lesions (Araujo‐Pires et al., 2014; Cavalla et al., 2021; Francisconi et al., 2016, 2018; Silva et al., 2017). It is noteworthy that systematic reviews of the literature have highlighted the suppressor potential of Tregs during the immune‐inflammatory response and their role in restoring alveolar bone homeostasis (Cafferata et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

TLR2 agonist prevents the progression of periapical lesions in mice by reducing osteoclast activity and regulating the frequency of Tregs

Almeida,

Battaglino,

Araujo

et al. 2024

Int Endodontic J

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AimTo evaluate the role of regulatory T lymphocytes (Tregs) in the presence or absence of the synthetic ligand Pam3Cys during the progression of periapical lesion in wild‐type (WT) and toll‐like receptor 2 knockout (TLR2KO) mice.Methodology.A total of 130 C57BL/6 male WT and TLR2KO mice were allocated into control (n = 5) and experimental (periapical lesion induction) (n = 10) groups. In specific groups (WT+Pam3cys and TLR2KO+Pam3cys), the synthetic ligand Pam3cys was administered intraperitoneally every 7 days, according to the experimental period (14, 21 and 42 days). At the end of those periods, the animals were euthanized, and the mandible and the spleen were submitted to histotechnical processing. Mandible histological sections were analysed by haematoxylin and eosin, TRAP histoenzymology and immunohistochemistry (FOXP3, RANK, RANKL and OPG). Spleen sections were analysed by immunohistochemistry (FOXP3).ResultsThe inflammatory infiltrate and bone resorption were more intense in the TLR2KO group compared to the WT group. The animals that received the Pam3cys had smaller periapical lesions when compared to the animals that did not receive the ligand (p < .05). TLR2KO animals showed a significant increase in the number of osteoclasts when compared to TLR2KO+Pam3cys group (p < .05). At 21 days, the WT+Pam3cys group had a lower number of osteoclasts when compared to the WT animals (p = .02). FOXP3 expression was more intense in the WT+Pam3cys groups when compared to the WT animals in the 42 days (p = .03). In the spleen analysis, the WT+Pam3cys group also had a higher expression of FOXP3 when compared to the WT animals at 14 and 42 days (p = .02). Concerning RANKL, there was a reduction in staining in the KOTLR2+Pam3cys groups at 21 and 42 days (p = .03) and a higher binding ratio between RANK/RANKL in animals that did not receive the ligand.ConclusionAdministration of the Pam3cys increased the proliferation of Tregs, showed by FOXP3 expression and prevented the progression of the periapical lesion in WT mice. On the other hand, in the TLR2KO animals, Treg expression was lower with larger areas of periapical lesions. Finally, systemic administration of the Pam3cys in KO animals was able to limit the deleterious effects of the absence of the TLR2 receptor.

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“…12 Additionally, Zou et al reviewed the role of regulatory T and B cells and their therapeutic application in periodontitis. 13 Probiotics are used for periodontitis treatment to upset periodontopathogens and to control the host's immune response to the presence of pathogenic microorganisms. [14][15][16] All these findings suggest that immunity has the potential to provide new ideas for the pathogenesis, diagnosis, and treatment of periodontitis.…”

Section: Introductionmentioning

confidence: 99%

“…Sirisereephap et al reviewed osteoimmunology in periodontitis and discussed the role of local proteins and promising synthetic compounds in alleviating periodontitis 12 . Additionally, Zou et al reviewed the role of regulatory T and B cells and their therapeutic application in periodontitis 13 . Probiotics are used for periodontitis treatment to upset periodontopathogens and to control the host's immune response to the presence of pathogenic microorganisms 14–16 .…”

Section: Introductionmentioning

confidence: 99%

Immune infiltration and diagnostic value of immune‐related genes in periodontitis using bioinformatics analysis

Lai

Wang

et al. 2023

J of Periodontal Research

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Background and Objectives Periodontitis, which is a chronic inflammatory periodontal disease resulting in destroyed periodontal tissue, is the leading cause of tooth loss in adults. Many studies have found that inflammatory immune responses are involved in the risk of periodontal tissue damage. Therefore, we analyzed the association between immunity and periodontitis using bioinformatics methods to further understand this disease. Materials and Methods First, the expression profiles of periodontitis and healthy samples were downloaded from the GEO database, including a training dataset GSE16134 and an external validation dataset GSE10334. Then, differentially expressed genes were identified using the limma package. Subsequently, immune cell infiltration was calculated by using the CIBERSORT algorithm. We further identified genes linking periodontitis and immunity from the ImmPort and DisGeNet databases. In addition, some of them were selected to construct a diagnostic model via a logistic stepwise regression analysis. Results and Conclusions Two hundred sixty differentially expressed genes were identified and found to be involved in responses to bacterial and immune‐related processes. Subsequently, immune cell infiltration analysis demonstrates significant differences in the abundance of most immune cells between periodontitis and healthy samples, especially in plasma cells. These results suggested that immunity doses play a non‐negligible role in periodontitis. Twenty‐one genes linking periodontitis and immunity were further identified. And nine hub genes of them were identified that may be key genes involved in the development of periodontitis. Gene ontology analyses showed that these genes are involved in response to molecules of bacterial origin, cell chemotaxis, and response to chemokines. In addition, three genes of them were selected to construct a diagnostic model. And its good diagnostic performance was demonstrated by the receiver operating characteristic curves, with an area under the curve of 0.9424 for the training dataset and 0.9244 for the external validation dataset.

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Immunotherapy with regulatory T and B cells in periodontitis

Cited by 13 publications

References 100 publications

Fibulin‐2: A potential regulator of immune dysfunction after bone trauma

Fibulin‐2: A potential regulator of immune dysfunction after bone trauma

TLR2 agonist prevents the progression of periapical lesions in mice by reducing osteoclast activity and regulating the frequency of Tregs

Immune infiltration and diagnostic value of immune‐related genes in periodontitis using bioinformatics analysis

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